A5012022583- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Banana Cultivation and Research
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Plant Disease Management Techniques
- Nanoparticles: synthesis and applications
- Plant Parasitism and Resistance
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Agricultural Practices and Plant Genetics
- Essential Oils and Antimicrobial Activity
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
- Nematode management and characterization studies
- Nanocomposite Films for Food Packaging
- Cassava research and cyanide
- Postharvest Quality and Shelf Life Management
- Insect-Plant Interactions and Control
University of Florida
2016-2025
Center for Surveillance, Epidemiology, and Laboratory Services
2024
Plant (United States)
2008-2023
Gainesville Obstetrics & Gynecology
2021
Pennsylvania State University
2016-2018
Connecticut Agricultural Experiment Station
2018
Florida Department of Agriculture and Consumer Services
2018
Southwest Florida Research
2006-2017
Florida Gulf Coast University
1988-2017
The Ohio State University
2015-2017
Abstract Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing ( HLB ). Breeding for disease‐resistant varieties most efficient sustainable approach to control plant diseases. Traditional breeding of challenging due multiple limitations, polyploidy, polyembryony, extended juvenility long crossing cycles. Targeted genome editing technology has potential shorten varietal development...
Significance Citrus bacterial canker, which is caused by several species in the genus Xanthomonas , a severe disease with worldwide distribution affecting all commercially important citrus and cultivars. The mechanisms of canker development, involving erumpent pustule formation growth, are not known. Our findings suggest that virulence determinants pathogens activate single host susceptibility (S) gene has critical contribution to growth development. S represents an excellent candidate for...
Citrus canker caused by Xanthomonas citri subspecies (Xcc) is a severe disease for most commercial citrus cultivars and responsible significant economic losses worldwide. Generating canker-resistant varieties will provide an efficient sustainable solution to control canker. Here, we report our progress in generating grapefruit modifying the PthA4 effector binding elements (EBEs) CsLOB1 Promoter (EBEPthA4 -CsLOBP) of (Citrus sinensis Lateral Organ Boundaries) gene. susceptibility gene induced...
Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; Species Xanthomonas euvesicatoria, vesicatoria, perforans and gardneri.Gram-negative, rod-shaped bacterium, aerobic, motile, single polar flagellum.Causes bacterial spot disease on plants belonging to the Solanaceae family, primarily tomato (Solanum lycopersicum), pepper (Capsicum annuum) chilli peppers frutescens).Necrotic lesions all above-ground plant...
ABSTRACT Although several reports underscore the importance of silicon (Si) in controlling Magnaporthe grisea on rice, no study has associated this beneficial effect with specific mechanisms host defense responses against fungal attack. In study, however, we provide evidence that higher levels momilactone phytoalexins were found leaf extracts from plants inoculated M. and amended (Si(+)) than not (Si(-) ) or noninoculated Si(+) Si(-) plants. On basis, more efficient stimulation terpenoid...
Acibenzolar-S-methyl (CGA 245704 or Actigard 50WG) is a plant activator that induces systemic acquired resistance (SAR) in many different crops to number of pathogens. was evaluated for management bacterial spot (Xanthomonas axonopodis pv. vesicatoria) and speck (Pseudomonas syringae tomato) tomato 15 7 field experiments, respectively. Experiments were conducted over 4-year period Florida, Alabama, North Carolina, Ohio, Ontario using local production systems. Applied at 35 g a.i. ha -1 ,...
ABSTRACT Although exogenous application of silicon (Si) confers efficient control rice blast, the probable hypothesis underlying this phenomenon has been confined to that a mechanical barrier resulting from Si polymerization in planta. However, study, we provide first cytological evidence Si-mediated resistance Magnaporthe grisea correlates with specific leaf cell reaction interfered development fungus. Accumulation an amorphous material stained densely toluidine blue and reacted positively...
Abstract Although ethylene regulates a wide range of defense-related genes, its role in plant defense varies greatly among different plant-microbe interactions. We compared ethylene's response to virulent and avirulent strains Xanthomonas campestrispv. vesicatoria tomato (Lycopersicon esculentum Mill.). The ethylene-insensitive Never ripe (Nr) mutant displays increased tolerance the strain, while maintaining resistance strain. Expression receptor genes NR andLeETR4 was induced by infection...
Abstract Background Bacterial spot of tomato and pepper is caused by four Xanthomonas species a major plant disease in warm humid climates. The are distinct from each other based on physiological molecular characteristics. genome sequence strain 85-10, member one the species, euvesicatoria ( Xcv ) has been previously reported. To determine relationship at level to investigate basis their virulence differing host ranges, draft genomic sequences members three were determined compared 85-10....
Protection of crops from bacterial diseases presents a continuing challenge, mandating the development novel agents and approaches. Photocatalysis is process where chemically reactive oxygen species are catalytically generated by certain minerals in presence light. These have capacity to destroy organic molecular structures critical pathogen viability. In this study, antibacterial potential photocatalytic nanoscale titanium dioxide (TiO(2)), TiO(2) doped (incorporation other materials into...
Xanthomonas campestris pv. vesicatoria, causal agent of bacterial spot tomato and pepper, had been considered for nearly 70 years to be a relatively homogeneous organism. However, in the past decade this bacterium was determined composed two genetically phenotypically distinct groups. The groups, designated A B, were distinguished based on amylolytic activity, expression unique protein bands, reaction differential hosts (tomato races T1 T2), patterns with monoclonal antibodies, DNA...
Bacteriophages are currently used as an alternative method for controlling bacterial spot disease on tomato incited by Xanthomonas campestris pv. vesicatoria. However, the efficacy of phage is greatly reduced due to its short residual activity plant foliage. Three formulations that significantly increased longevity surface were tested in field and greenhouse trials: (i) PCF, 0.5% pregelatinized corn flour (PCF) + sucrose; (ii) Casecrete, Casecrete NH-400 sucrose 0.25% PCF; (iii) skim milk,...
Various combinations of the harpin protein, acibenzolar-S-methyl, and bacteriophages were compared for controlling tomato bacterial spot in field experiments. Harpin protein aciben-zolar-S-methyl applied every 14 days beginning twice before transplanting then an additional four applications throughout season. Formulated prior to inoculation followed by a week at dusk. A standard bactericide treatment, consisting copper hydroxide plus mancozeb, was once 7 days, while untreated plants served...
Although there are adequate DNA sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of phenotype challenge. The problem the absence taxonomy describes genetic variation biologically relevant resolution database containing reference strains comparison. Moreover, evolution, population genetics, ecology, epidemiology many still poorly understood. To address...
C5 volatile compounds, derived from fatty acids, are among the most important contributors to consumer liking of fresh tomatoes. Despite their roles in flavour, genes responsible for synthesis have yet be identified. This work shows that is catalysed part by a 13-lipoxygenase (LOX), TomloxC, same enzyme C6 volatiles. independent hydroperoxide lyase (HPL); moreover, HPL knockdown significantly increased synthesis. LOX-dependent, HPL-independent pathway functions both fruits and leaves....
Bacterial spot disease of pepper and tomato is caused by four distinct Xanthomonas species a severely limiting factor on fruit yield in these crops. The genetic diversity the type III effector repertoires large sampling field strains for this have yet to be explored genomic scale, our understanding pathogen evolution an agricultural setting. Genomes sixty-seven euvesicatoria (Xe), perforans (Xp), gardneri (Xg) isolated from diseased fields southeastern midwestern United States were sequenced...