A5052777607- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Gut microbiota and health
- Nematode management and characterization studies
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Clostridium difficile and Clostridium perfringens research
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Insect symbiosis and bacterial influences
- Cocoa and Sweet Potato Agronomy
- Coastal wetland ecosystem dynamics
- Bacteriophages and microbial interactions
- Mycorrhizal Fungi and Plant Interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Agronomic Practices and Intercropping Systems
- Microbial infections and disease research
- Plant Disease Resistance and Genetics
- Lung Cancer Diagnosis and Treatment
- Studies on Chitinases and Chitosanases
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
Yangzhou University
2020-2025
Florida Department of Citrus
2016-2024
University of Florida
2015-2024
Shanghai Public Health Clinical Center
2021-2024
Shandong University
2022-2024
Second Hospital of Shandong University
2024
First Affiliated Hospital of Nanchang University
2022
Nanchang University
2022
Shandong Chest Hospital
2015-2022
China Agricultural University
2010-2017
Abstract Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing ( HLB ). Breeding for disease‐resistant varieties most efficient sustainable approach to control plant diseases. Traditional breeding of challenging due multiple limitations, polyploidy, polyembryony, extended juvenility long crossing cycles. Targeted genome editing technology has potential shorten varietal development...
Citrus is a globally important, perennial fruit crop whose rhizosphere microbiome thought to play an important role in promoting citrus growth and health. Here, we report comprehensive analysis of the structural functional composition microbiome. We use both amplicon deep shotgun metagenomic sequencing bulk soil samples collected across distinct biogeographical regions from six continents. Predominant taxa include Proteobacteria, Actinobacteria, Acidobacteria Bacteroidetes. The core...
Abstract The root microbes play pivotal roles in plant productivity, nutrient uptakes, and disease resistance. microbial community structure has been extensively investigated by 16S/18S/ITS amplicons metagenomic sequencing crops model plants. However, the functional associations between host growth are poorly understood. This work investigates bacterial of foxtail millet (Setaria italica) its potential effects on productivity. We determined composition 2882 samples from rhizoplane,...
The rhizobium–legume symbiosis has been widely studied as the model of mutualistic evolution and essential component sustainable agriculture. Extensive genetic recent genomic studies have led to hypothesis that many distinct strategies, regardless rhizobial phylogeny, contributed varied symbiosis. We sequenced 26 genomes Sinorhizobium Bradyrhizobium nodulating soybean test this hypothesis. core genome is disproportionally enriched in lipid secondary metabolism, whereas several gene clusters...
Roots are the primary site for plant-microbe interactions. Among three root-associated layers (i.e., rhizosphere, rhizoplane, and endorhiza), rhizoplane is a key component serving critical gating role that controls microbial entry into plant roots. The communities colonizing believed to be gradually enriched from bulk soil inoculum. However, it unknown how this enrichment process, particularly rhizosphere step, affected by biotic stresses, such as disease. In study, we address question using...
Plants can recruit beneficial microbes to enhance their ability defend against pathogens. However, in contrast the intensively studied roles of rhizosphere microbiome suppressing plant pathogens, collective community-level change and effect phyllosphere response pathogen invasion remains largely elusive.Here, we integrated 16S metabarcoding, shotgun metagenomics culture-dependent methods systematically investigate changes between infected uninfected citrus leaves by Diaporthe citri, a fungal...
As the putative center of origin for soybean and second largest region production in China, North China Plain covers temperate subtropical regions with diverse soil characteristics. However, rhizobia this plain have not been sufficiently studied. To investigate biodiversity biogeography plain, a total 309 isolates symbiotic bacteria from nodules collected 16 sampling sites were studied by molecular characterization. These classified into 10 genospecies belonging to genera Sinorhizobium...
SaCas9/sgRNA, derived from Staphylococcus aureus, is an alternative system for genome editing to Streptococcus pyogenes SpCas9/sgRNA. The smaller SaCas9 recognizes a different protospacer adjacent motif (PAM) sequence SpCas9. SaCas9/sgRNA has been employed edit the genomes of Arabidopsis, tobacco and rice. In this study, we aimed test its potential in citrus. Transient expression Duncan grapefruit via Xcc-facilitated agroinfiltration showed it can successfully modify CsPDS Cs2g12470....
Abstract Metagenomic binning is an essential technique for genome-resolved characterization of uncultured microorganisms in various ecosystems but hampered by the low efficiency tools adequately recovering metagenome-assembled genomes (MAGs). Here, we introduce BASALT ( B inning A cross a S eries ssemb l ies T oolkit) and refinement short- long-read sequencing data. employs multiple binners with thresholds to produce initial bins, then utilizes neural networks identify core sequences remove...
Citrus Huanglongbing (HLB) is the most destructive citrus disease worldwide. HLB associated with three species of phloem-limited, gram-negative, fastidious α-proteobacteria: Candidatus Liberibacter asiaticus (Las), Ca. L. americanus (Lam), and africanus (Laf) Las being widespread species. has not been cultured in artificial media, which greatly hampered our efforts to understand its virulence mechanisms. contains a complete Sec-translocon, suggested transport proteins including factors into...
Defects in gut barrier function are implicated gastrointestinal (GI) disorders like inflammatory bowel disease (IBD), as well systemic inflammation. With the increasing incidence of IBD worldwide, more attention should be paid to dietary interventions and therapeutics with potential boost natural defense mechanisms epithelial cells. The current study aimed investigate protective effects Limosilactobacillus reuteri ATCC PTA 4659 a colitis mouse model delineate behind it. Wild-type mice were...
In a survey of the biodiversity and biogeography rhizobia associated with soybean (Glycine max L.) in different sites Northern (Huang-Huai-Hai) Plain China, ten strains were defined as representing novel genomic species genus Bradyrhizobium. They distinguished from restriction fragment length polymorphism (RFLP) analysis 16S rRNA gene 16S-23S intergenic spacer (IGS). BOX-PCR, these presented two patterns that shared 94% similarity, demonstrating they homogenous group limited diversity....
The microbiome associated with crop plants has a strong impact on their health and productivity. Candidatus Liberibacter asiaticus (Las), the bacterial pathogen responsible for Huanglongbing (HLB) disease, lives inside phloem of citrus including root system. It been suggested that Las negatively affects microbiome. On other hand, members also influence interaction between citrus. Here, we report isolation characterization multiple putative beneficial bacteria from healthy rhizosphere....
Xanthomonas citri subsp. pathotypes cause bacterial citrus canker, being responsible for severe agricultural losses worldwide. The A pathotype has a broad host spectrum, while A* and Aw are more restricted both in hosts geography. Two previous phylogenomic studies led to contrasting well-supported clades sequenced genomes of these pathotypes. No extensive biogeographical or divergence dating analytic approaches have been so far applied available genomes.Based on larger sampling than...
Phenol is a common environmental contaminant. The purpose of this study was to isolate phenol-degrading microorganisms from wastewater in the sections Chinese Medicine Manufactory. Acinetobacter lwoffii NL1 identified based on combination biochemical characteristics and 16S rRNA genes. To analyze molecular mechanism, whole genome A . sequenced, yielding 3499 genes one circular chromosome three plasmids. Enzyme activity analysis showed that degraded phenol via ortho -cleavage rather than meta...
Citrus HLB caused by Candidatus Liberibacter asiaticus is a pathogen-triggered immune disease. Here, we identified putative genetic determinants of pathogenicity integrating citrus genomic resources to characterize the pan-genome accessions that differ in their response HLB. Genome-wide association mapping and analysis allele-specific expression between susceptible, tolerant, resistant further refined candidates underlying We first developed phased diploid assembly sinensis 'Newhall' genome...
In our previous microbiome profiling analysis,
Thirteen bacterial isolates from root nodules of soybean grown in saline-alkaline soils the Chinese province Hebei were identified as a unique group genus Ensifer based upon BOX-PCR patterns, sequencing analyses 16S rRNA and housekeeping genes DNA–DNA hybridization. Phenotypically, positive tests for acid production negative results reduction litmus milk sensitivity to 50 µg ampicillin ml −1 , well some other features, could differentiate novel defined species – Sinorhizobium group. The had...