A5024030510- Rice Cultivation and Yield Improvement
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- GABA and Rice Research
- Plant Reproductive Biology
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Agriculture, Soil, Plant Science
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Biotin and Related Studies
Chongqing Normal University
2017-2025
Wuhan University
2011-2016
State Key Laboratory of Hybrid Rice
2014
Abstract The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. molecular mechanism involved Rf gene–mediated processing CMS-associated transcripts is unclear, as are identities other proteins that may CMS–Rf interaction. In this study, we cloned gene Rf5 for Hong-Lian CMS rice and studied its restoration with respect to transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable bind...
The pentatricopeptide repeat (PPR) protein family is a large characterized by tandem arrays of degenerate 35-amino-acid motif whose members function as important regulators organelle gene expression at the post-transcriptional level. Despite roles PPRs in RNA editing organelles, their activities and underlying mechanism remain obscure. Here, we show that novel DYW motif-containing PPR protein, PPS1, associated with five conserved RNA-editing sites nad3 located close proximity to each other...
In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA mitochondria and chloroplasts, which is critical for plant development evolutionary adaption. Although studies showed number PPR generally influence editing organellar genes, few them were characterized detail rice. Here, we report PLS-E subclass protein rice, PPR756, loss function led to abolishment events among three mitochondrial genes including atp6, ccmC,...
<title>Abstract</title> Perennial Chinese rice is a novel type of germplasm native to China. This study comprehensively evaluated the variation in agronomic traits 20 perennial across four different planting seasons explore genetic diversity and effectively utilize them. A total 16 traits, including heading date, plant height, thousand-grain weight, were investigated based on field phenotypic values. The findings revealed significant variations among these with broad range Shannon–Wiener...
Summary Nitrogen (N) is critical for crop growth, and enhancing nitrogen use efficiency (NUE) essential achieving high grain yields quality in production. In this study, we identified two transcription factors OsRF2b OsbZIP61, which localize the nucleus function as negative regulators of NUE yield rice. responds to supplies, overexpression resulted growth retardation, reduced content, lower transgenic lines, leading decreased yield. contrast, rf2b mutant exhibited increased plant height,...
Summary The restoration fertility complex ( RFC ) was previously identified in Honglian HL )‐cytoplasmic male sterility CMS rice Oryza sativa ), and glycine‐rich protein 162 GRP 162) is responsible for binding to the ‐associated transcript atp6‐orfH79 . Here, we engineered a recombinant containing mitochondrial transit peptide, termed Mt‐ 162, as an artificial restorer of Rf gene. confirmed bind RNA localize mitochondria. transgenic plants showed restored with partially functional pollen. We...
Summary Cytoplasmic male sterility ( CMS ) and restoration of fertility Rf are widely distributed in plant species utilized by humans. RF 5 GRP 162 subunits the complex RFC Hong‐Lian rice. Despite fact that is 400–500 kDa size, other proteins or factors still remain unknown. Here, we identified subunit 3, which encodes a DUF 1620‐containing WD 40‐like repeat protein 3) present all tissues but highly expressed leaves. We established 3 interacts with both vitro vivo , transported into...
Abstract U nderstanding the field performance on grain yield and quality genetic diversity of overwintering (OW) cultivated rice ( Oryza sativa L.) across main crop (MC) ratooning (RC) is premise to make strategies for future OW variety improvement in production. The present experiments were conducted RC 2016, MC both 2017 2018, 2019 identify genotypes that perform stable terms different climate conditions. plant -1 (GYP) its components six exhibited significant difference 4 years (P ≤...
Transcription factors (TFs) are indispensable components of transcriptional regulatory pathways involved in crop growth and development. Herein, we developed a new method for the identification upstream TFs specific to genes crops based on binding affinities biotin avidin. First, constructed verified avidin system (BAS) by coprecipitation assay. Subsequently, feasibility DNA-based BAS (DBAS) was further proved vivo vitro assays. Furthermore, cloned promoter rice OsNRT1.1B possible regulators...
The rice mutant mp3 was derived from an indica–japonica cross between Rejing35 and XieqingzaoB, producing inconstant number of pistils ranging one to four in a floret at heading stage, which also developed open-glume with or two seeds twin seedlings mature seedling stage. Several altered characteristics, including filling grain panicle–1 (62.90), grain-setting rate (60.48%) yield plant–1 (13.42 g), decreased but increase 1000-grain weight (36.87 g) observed. Genetic analysis revealed that...
Improving plant nitrogen use efficiency (NUE) is important for many crops, especially in hybrid breeding. Reducing inputs key achieving sustainable rice production and decreasing environmental problems. Here, we analyzed the transcriptomic physiological changes two indica restorer lines (Nanhui511 Minghui23) under high (HN) low (LN) conditions. Nanhui511 (NH511) was more sensitive to different supplies exhibited higher uptake NUE HN conditions by increasing lateral root tiller numbers...
Abstract In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA mitochondria and chloroplasts, which is critical for plant development evolutionary adaption. Although studies showed number PPR generally influence editing organellar genes, few them were characterized detail rice. Here, we report PLS-E subclass protein rice, PPR756, loss function led to abolishment events among three mitochondrial genes including atp6,...
Improving plant nitrogen-use efficiency (NUE) has great significance for various crops, particularly in hybrid breeding. Reducing nitrogen inputs is key to achieving sustainable rice production and mitigating environmental problems. In this study, we analyzed the transcriptomic physiological changes two indica restorer lines (Nanhui511 [NH511] Minghui23 [MH23]) under high (HN) low (LN) conditions. Compared MH23, NH511 was more sensitive different supplies exhibited higher uptake NUE HN...
This study aimed to explore the genetic information about yield traits in perennial Chinese rice "Shendao3#" and provide a series of theoretical bases for innovative utilization germplasm. The F2 population derived from cross between one annual "XiqingzaoB" was developed identify QTLs&rsquo; underlying yield-related traits. Fifteen including plant height, panicle plant-1, thousand-grain weight were measured QTL mapping. software packages Excel 2010, GraphpadPrism 5.0, QTLIciMapping 4.10...
<title>Abstract</title> "Shengdao3<sup>#</sup>" is a novel type of perennial rice germplasm native to China. And so, this study aimed explore the genetic information about yield traits in Chinese and provide series theoretical bases for innovative utilization germplasm. The F<sub>2</sub> population derived from cross between common "XiqingzaoB" was developed identify QTLs underlying yield-related traits. Fifteen including plant height, panicle plant<sup>− 1</sup>, thousand-grain weight were...
Segregation distortion is a common phenomenon that has been observed in genetics and plant breeding; however, the mechanism of segregation unknown. In present study, three half-sib F2 populations derived from japonica overwinter (perennial) rice varieties (W1, W2, W3) crossed to indica variety Minghui725 (MH725) were developed construct linkage maps. We established map lengths 2032.8, 2317.4, 2108.7 cM with average intervals 20.1, 20.5, 19.7 using 101, 113, 107 SSR markers W1/MH725,...
Rice productivity relies heavily on nitrogen fertilization, and improving use efficiency (NUE) is important for hybrid rice breeding. Reducing inputs the key to achieving sustainable production reducing environmental problems. Here, we analyzed genome-wide transcriptomic changes in microRNAs (miRNAs) indica restorer cultivar Nanhui 511 (NH511) under high (HN) low (LN) conditions. The results showed that NH511 sensitive supplies HN conditions promoted growth its lateral roots at seedling...