A5080959286- Phytoplasmas and Hemiptera pathogens
- Atomic and Molecular Physics
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Advanced Chemical Physics Studies
- Banana Cultivation and Research
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Insect-Plant Interactions and Control
- X-ray Spectroscopy and Fluorescence Analysis
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Insect Resistance and Genetics
- Electron and X-Ray Spectroscopy Techniques
- Pineapple and bromelain studies
- Spectroscopy and Laser Applications
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- Atmospheric Ozone and Climate
Agronomical Institute of Campinas
2015-2024
Princeton University
2024
Bernardino Rivadavia Natural Sciences Museum
2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2024
Hospital de Clínicas
2023
Hospital São Rafael
2023
Complexo Hospitalar Universitário Professor Edgard Santos
2023
Universidade Federal da Bahia
2023
Karolinska Institutet
2022
Universidade Federal de São Carlos
2011-2021
Genome sequences of nine species citrus, including oranges, pummelos and mandarins, reveal pathways domestication provide resources for breeding. Cultivated citrus are selections from, or hybrids of, wild progenitor whose identities contributions to remain controversial. Here we sequence compare genomes—a high-quality reference haploid clementine genome mandarin, pummelo, sweet-orange sour-orange genomes—and show that cultivated types derive from two species. Although represent one species,...
Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely used technique for expression profiling of selected genes. However, obtaining reliable measurements depends on the selection appropriate reference genes gene normalization. The aim this work was to assess stability 15 candidate determine which set is best suited transcript normalization in citrus different tissues organs leaves challenged with five pathogens (Alternaria alternata, Phytophthora parasitica,...
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
Huanglongbing (ex-greening) disease is one of the most serious diseases citrus. It caused by phloem-limited, gram-negative bacterium "Candidatus Liberibacter spp.". This not well characterized mainly because it still uncultured. There are two known strains, Asian ("Candidatus asiaticus") and African africanus") that cause severe damage to citrus plants including twig dieback, decline, death. Symptoms first appear as leaf mottling chlorosis occurring in shoot or sector trees. Later, symptoms...
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...
The complete nucleotide sequence of the genomic RNA 1 (8745 nt) and 2 (4986 Citrus leprosis virus cytoplasmic type (CiLV-C) was determined using cloned cDNA. contains two open reading frames (ORFs), which correspond to 286 29 kDa proteins. protein is a polyprotein putatively involved in replication, four conserved domains: methyltransferase, protease, helicase polymerase. ORFs corresponding 15, 61, 32 24 proteins, respectively. apparently cell-to-cell movement virus, but none other putative...
Citrus fruits (sweet orange, mandarin, pummelo, grapefruit, lemon, lime and assorted hybrids) are among the most widely grown economically important fruit tree crops in world. As fresh fruit, they an nutrient dense food source for human diets; as processed juice products, primarily sweet orange juice, represent a globally traded commodity. To support genetic improvement efforts this crop, international citrus genetics community has collaborated with sequencing centers development of freely...
Citrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and americanus (CaLam), depending on the continent where were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas CaLam both species are present in Brazil. Several studies of transcriptional response plants manifesting HLB symptoms have been reported, but only for infection....
Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity this associated with its capacity to colonize and form biofilm the xylem vessels host plants, there not yet any method directly reduce populations field. study, we investigated inhibitory effect N-Acetylcysteine (NAC), cysteine analogue used mainly treat human diseases, on X. experimental conditions. Concentrations NAC...
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important pests. ACP vector phloem-limited bacteria Candidatus Liberibacter americanus and asiaticus, causal agents devastating disease huanglongbing (HLB). management HLB based on use healthy young plants, eradication infected plants chemical control vector. RNA interference (RNAi) has proven to be a promising tool pests explore gene functions. Recently, studies have reported that target mRNA knockdown in many...
Abstract Background Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa , is one the most important citrus diseases, and affects all varieties of sweet orange ( sinensis L. Osb). On other hand, among genus there are different sources resistance against X. . For these species identifying defense genes could be an step towards obtaining resistant through breeding or genetic engineering. To assess we made use mandarin C. reticulata Blanco) that known to CVC shares agronomical...
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and evaluate the efficiency these for characterization sweet orange. We developed SSRs from a genomic library 'Pêra IAC' orange enriched AG/TC, GT/CA, TCA/AGT AAC/TTG repeats. selected 279 sequences which 171 primer pairs designed 113 with best banding patterns selected. Characterization loci revealed that AG/TC was most abundant (69%) class isolated, followed by GT/CA (15.9%),...
A biofilm is a community of microorganisms attached to solid surface. Cells within biofilms differ from planktonic cells, showing higher resistance biocides, detergent, antibiotic treatments and host defense responses. Even though there are number gene expression studies in bacterial formation, limited information available concerning plant pathogen. It was previously demonstrated that the pathogen Xylella fastidiosa could grow as biofilm, possibly important factor for its pathogenicity. In...
Gummosis and root rot caused by Phytophthora are among the most economically important diseases in citrus. Four F1 resistant hybrids (Pool R), four susceptible S) to P. parasitica, were selected from a cross between Citrus sunki Poncirus trifoliata cv. Rubidoux. We investigated gene expression pools of comparison with their parents 48 hours after parasitica inoculation. proposed that genes differentially expressed provide promising candidates for identifying transcripts involved disease...
Citrus sudden death (CSD) has caused the of approximately four million orange trees in a very important citrus region Brazil. Although its etiology is still not completely clear, symptoms and distribution affected plants indicate viral disease. In search for viruses associated with CSD, we have performed comparative high-throughput sequencing analysis transcriptome small RNAs from CSD-symptomatic -asymptomatic using Illumina platform. The data revealed mixed infections that included tristeza...
Citrus breeding programs have many limitations associated with the species biology and physiology, requiring incorporation of new biotechnological tools to provide possibilities. Diversity Arrays Technology (DArT) markers, combined next-generation sequencing, wide applicability in construction high-resolution genetic maps quantitative trait locus (QTL) mapping. This study aimed construct an integrated map using full-sib progeny derived from Murcott tangor Pera sweet orange DArTseq™ molecular...