Gummosis and root rot caused by Phytophthora are among the most economically important diseases in citrus. Four F1 resistant hybrids (Pool R), four susceptible S) to P. parasitica, were selected from a cross between Citrus sunki Poncirus trifoliata cv. Rubidoux. We investigated gene expression pools of comparison with their parents 48 hours after parasitica inoculation. proposed that genes differentially expressed provide promising candidates for identifying transcripts involved disease...

Citrus breeding programs have many limitations associated with the species biology and physiology, requiring incorporation of new biotechnological tools to provide possibilities. Diversity Arrays Technology (DArT) markers, combined next-generation sequencing, wide applicability in construction high-resolution genetic maps quantitative trait locus (QTL) mapping. This study aimed construct an integrated map using full-sib progeny derived from Murcott tangor Pera sweet orange DArTseq™ molecular...

Abstract Huanglongbing (HLB), caused mainly by ‘ Candidatus Liberibacter asiaticus’ (CLas), is the most devastating citrus disease because all commercial species are susceptible. HLB tolerance has been observed in Poncirus trifoliata and their hybrids. A wide-ranging transcriptomic analysis using contrasting genotypes regarding severity was performed to identify genetic mechanism associated with HLB. The included Citrus sinensis, sunki, three distinct groups of hybrids obtained from crosses...

Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were in unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of non-redundant ESTs contained SSRs. The frequencies different SSR motifs similar between spp trifoliate orange. In general, mononucleotide repeats appeared to be most abundant SSRs database, but we also identify di-, tri-, tetra-, penta- hexanucleotide repeats....

Huanglongbing (HLB) is a destructive disease of citrus caused by phloem-limited bacteria, namely 'Candidatus Liberibacter asiaticus' (Las), africanus', and americanus'. Although there are no known HLB-resistant species, studies have reported Poncirus trifoliata as being more tolerant. Assuming that callose deposition in the phloem infected plants can inhibit translocation photosynthetic products cause starch accumulation, we compared petioles accumulation leaves three genotypes (Citrus...

Tahiti lime is one of the most exported fruits in Brazil, and state São Paulo accounts for more than half country’s fruit production, drought region a growing concern as rainfall distribution has become increasingly unpredictable. Therefore, identifying best varieties management strategies citrus groves subject increasing research. This experiment was conducted at “Sylvio Moreira” Citrus Center, Brazil; focused on different planting mulching practices scion-rootstock combinations. Two...

Rootstock diversification is increasingly desired and necessary for the sustainability of citriculture, which subject to adversity, such as occurrence Huanglongbing (HLB), has impacted activity in most producing regions world. The objective was evaluate productivity, water-use efficiency, vegetative growth, HLB incidence Valencia sweet orange grafted onto three citrandarins (dwarfing) Swingle citrumelo (standard). field experiment conducted under a high-density planting spacing 5.0 m × 1.5...

The selection of new mandarin varieties to enhance fruit supply and address phytosanitary challenges is a significant issue in table citrus farming. In Brazil, the primary harvest season for commercial spans from May August, leaving gap availability mandarins during off-season, which typically coincides with hottest months year. Furthermore, diseases such as Alternaria Brown Spot (ABS) HLB have reduced planted areas due complexities management increased production costs. This study evaluated...

Although the citriculture is one of most important economic activities in Brazil, it based on a small number varieties. This fact has contributed for vulnerability culture regarding phytosanitary problems. A higher varieties/genotypes with potential commercial growing, either industry or fresh market, been main objectives citrus breeding programs. The genetic improved, last decades, due to possibility an association between biotechnological tools and classical methods breeding. use molecular...

Obtaining new rootstocks that have resistance to biotic and abiotic factors is one of the main goals breeding programs for citrus. This study evaluated performance 42 hybrids Sunki mandarin (Citrus sunki Hort. ex Tanaka) vs. Poncirus trifoliata cv. Rubidoux, as rootstock Pera sweet orange. The experiment was conducted in Colômbia, São Paulo, Brazil, randomized blocks with three replications a spacing 6.0 m x 3.5 m. trees were seven years old without irrigation. We quantified variables...

Huanglongbing (HLB), caused by the bacterium 'Candidatus Liberibacter' spp., is currently one of most serious diseases citrus plants and has substantial economic losses. Thus far, there no source genetic resistance to HLB in genus Citrus or its relatives. However, several studies have reported Poncirus trifoliata some hybrids be more tolerant disease. The main objective this study was report differences incidence 'Ca. L. asiaticus' infection citrandarin plants, from Sunki mandarin (Citrus...

15 (4): gmr15048863 its effectiveness for phenotype prediction.The proportion of genetic variance explained by the markers was also computed.The heritability traits, as determined markers, 16-28%.The predictive ability these ranged from 0.53 to 0.64, and regression coefficients between predicted observed phenotypes were close unity.Over 35% accounted markers.Accuracy estimates with GWS lower than those obtained phenotypic analysis; however, superior in terms gain per unit time.Thus, may be...

Citrus is the most important fruit crop in Brazil and tristeza virus (CTV) considered one of pathogens citrus. Most citrus species varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative citrus, resistant virus. In order better understand responses plants infection CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from trifoliata plants, inoculated or not at 90 days after inoculation, grafted on Rangpur lime rootstocks. We...

Phytophthora nicotianae Breda de Haan (Phytophthora parasitica Dastur) causes severe damage to citrus crops worldwide. A population of citrandarins was created from the cross between susceptible parent Citrus sunki Hort. Ex Tan. and resistant Poncirus trifoliata (L.) Raf. cv. Rubidoux, both parents two reference rootstocks (Rangpur lime Swingle citrumelo) were grafted in a greenhouse on Rangpur lime. Inoculations performed at 10 cm 15 above grafting region resulting lesions evaluated by...

ABSTRACT Alternaria brown spot (ABS) disease is caused by the fungus of alternata f. sp. citri, which causes injury in leaves, branches and fruits citrus. The action pathogen directly related to presence toxin receptors susceptible genotypes. objective this study was characterize a population citrus hybrids obtained from controlled crosses between Pêra de Abril sweet orange hybrid Murcott tangor x (TM LP 163) for response ABS through vitro inoculation fungal spores young detached leaves....

Resistance to Xylella fastidiosa was evaluated in 264 hybrids of crosses between Murcott tangor (Citrus reticulata × Citrus sinensis) and Pera sweet orange (C. under field conditions. Uninfected were grafted with buds collected from plants infected X. fastidiosa, forming a plant two scions (i.e., hybrid branches branches). From these plants, we chose 10 genotypes three biological replicates. We gene expression, bacterial multiplication, citrus variegated chlorosis (CVC) symptom development...

O objetivo deste trabalho foi obter plantas autotetraplóides de tangerina 'Ponkan', laranja 'Pêra-de-abril' e tangor 'Murcott', que serão usadas em cruzamentos com cultivares diplóides, visando à obtenção indivíduos triplóides sem sementes. Utilizou-se o método cultivo in vitro segmentos epicótilo meio colchicina (0,025, 0,05 0,1%), por diversos períodos (1, 2, 3, 7 14 dias), subseqüente regeneração brotações a presença do alcalóide. As foram microenxertadas aclimatizadas estufas. A...