Citrus leprosis is considered the main viral disease for Brazilian citrus production, particularly State of São Paulo, due to high costs spent chemical control its vector, tenuipalpid mite Brevipalpus phoenicis. In addition, global importance has significantly increased in last years, with dissemination virus new countries South and Central America. Brazil, despite economical occurrence more than seven decades, most significant advances towards understanding pathosystem interactions have...

Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were in unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of non-redundant ESTs contained SSRs. The frequencies different SSR motifs similar between spp trifoliate orange. In general, mononucleotide repeats appeared to be most abundant SSRs database, but we also identify di-, tri-, tetra-, penta- hexanucleotide repeats....

Tahiti lime is one of the most exported fruits in Brazil, and state São Paulo accounts for more than half country’s fruit production, drought region a growing concern as rainfall distribution has become increasingly unpredictable. Therefore, identifying best varieties management strategies citrus groves subject increasing research. This experiment was conducted at “Sylvio Moreira” Citrus Center, Brazil; focused on different planting mulching practices scion-rootstock combinations. Two...

Rootstock diversification is increasingly desired and necessary for the sustainability of citriculture, which subject to adversity, such as occurrence Huanglongbing (HLB), has impacted activity in most producing regions world. The objective was evaluate productivity, water-use efficiency, vegetative growth, HLB incidence Valencia sweet orange grafted onto three citrandarins (dwarfing) Swingle citrumelo (standard). field experiment conducted under a high-density planting spacing 5.0 m × 1.5...

The selection of new mandarin varieties to enhance fruit supply and address phytosanitary challenges is a significant issue in table citrus farming. In Brazil, the primary harvest season for commercial spans from May August, leaving gap availability mandarins during off-season, which typically coincides with hottest months year. Furthermore, diseases such as Alternaria Brown Spot (ABS) HLB have reduced planted areas due complexities management increased production costs. This study evaluated...

The randomly amplified polymorphic DNA (RAPD) technique was used to distinguish nucellar and zygotic seedlings resulting from crosses between the ‘Montenegrina’ (Citrus deliciosa Tenore) and‘ King’ (C. nobilis Loureiro) tangerines. aim of present study develop tangerine varieties with a reduced number seeds organoleptic characteristics similar tangerine. Embryos were isolated seeds, cultivated in vitro, acclimated greenhouse. Four random primers identify 54 plants sexual origin total 202...

A muda cítrica é o insumo mais importante na formação de um pomar. As características importantes da são a origem do enxerto e porta-enxerto, qualidade sistema radicular sua sanidade. Os porta-enxertos capazes influenciar várias horticulturais sanitárias nas árvores nos frutos cítricos, como: sólidos solúveis totais, tamanho copa fruto, resistência moléstias ao frio, distribuição raízes, etc. O principal porta-enxerto utilizado no Brasil (cerca 80%) limoeiro ‘Cravo’ (Citrus limonia Osbeck...

Although the citriculture is one of most important economic activities in Brazil, it based on a small number varieties. This fact has contributed for vulnerability culture regarding phytosanitary problems. A higher varieties/genotypes with potential commercial growing, either industry or fresh market, been main objectives citrus breeding programs. The genetic improved, last decades, due to possibility an association between biotechnological tools and classical methods breeding. use molecular...

Obtaining new rootstocks that have resistance to biotic and abiotic factors is one of the main goals breeding programs for citrus. This study evaluated performance 42 hybrids Sunki mandarin (Citrus sunki Hort. ex Tanaka) vs. Poncirus trifoliata cv. Rubidoux, as rootstock Pera sweet orange. The experiment was conducted in Colômbia, São Paulo, Brazil, randomized blocks with three replications a spacing 6.0 m x 3.5 m. trees were seven years old without irrigation. We quantified variables...

Citrus leprosis (CL) is the main viral disease affecting Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm observed nucleus infected cells symptomatic tissues. RNA extracts from three plants, which proved negative RT-PCR for known CL-causing viruses, analyzed high throughput sequencing Sanger after RT-PCR. The genomes bi-segmented ss(-)RNA...

15 (4): gmr15048863 its effectiveness for phenotype prediction.The proportion of genetic variance explained by the markers was also computed.The heritability traits, as determined markers, 16-28%.The predictive ability these ranged from 0.53 to 0.64, and regression coefficients between predicted observed phenotypes were close unity.Over 35% accounted markers.Accuracy estimates with GWS lower than those obtained phenotypic analysis; however, superior in terms gain per unit time.Thus, may be...

Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in Brazilian citrus industry. This occurs because of widespread source inoculum and year-round presence vector, tenuipalpid mite Brevipalpus phoenicis, plants. In addition, while some species are resistant to CiLV-C, C. sinensis, cultivated country, extremely susceptible disease. The objective this work was identify genes sinensis cv. Pêra plants that were differentially expressed after host...

There are two bacilliform, rhabdo-like viruses that cause citrus leprosis: Citrus leprosis virus C (CiLV-C), which accumulates in the cytoplasm of infected cells, and nuclear type (CiLV-N), their nucleus. The first one, prototype new genus Cilevirus, is prevalent occurs several countries American continent, from Argentina to Mexico (1). second type, still a tentative member Rhabdoviridae family, rare occurrence, with few reports Brazil one Panama Leprosis particularly important Brazilian...

A severidade da mancha-marrom de alternária nos pomares brasileiros tangerinas tem causado sérias preocupações aos citricultores, devido prejuízos em plantios comerciais variedades suscetíveis, como a tangerina Ponkan e o tangor Murcott. Para avaliar resposta diferentes genótipos ao fungo, foram realizadas inoculações Alternaria alternata in vitro vivo, 54 seus híbridos, selecionados no Banco Ativo Germoplasma Citros do Centro APTA Sylvio Moreira, Instituto Agronômico, Cordeirópolis-SP,...

ABSTRACT Alternaria brown spot (ABS) disease is caused by the fungus of alternata f. sp. citri, which causes injury in leaves, branches and fruits citrus. The action pathogen directly related to presence toxin receptors susceptible genotypes. objective this study was characterize a population citrus hybrids obtained from controlled crosses between Pêra de Abril sweet orange hybrid Murcott tangor x (TM LP 163) for response ABS through vitro inoculation fungal spores young detached leaves....

The genetic inheritance of resistance to leprosis, the most important viral disease citrus in Brazil, was characterized through phenotypic assessment 143 hybrids resulting from crosses between tangor ‘Murcott’ (Citrus sinensis × C. reticulata) and sweet orange ‘Pêra’ (C. sinensis), considered be resistant susceptible disease, respectively. All plants were grafted onto Rangpur lime limonia) inoculated with Citrus leprosis virus, cytoplasmic type infestation viruliferous mites, Brevipalpus...

Alternaria brown spot, or ABS (Alternaria alternata), is the most prevalent fungal disease of tangerines in world. Field observations have revealed lesions on leaves wounded by citrus leaf miner (CLM). Thus, objective this study was to assess relationship between CLM and ABS. Leaves from young Murcott tangor plants detached 16 different varieties with without caused were inoculated A. alternata. The symptoms subsequently quantified counting number lesions, lesion area estimated using a...

Sour rot, caused by Geotrichum citri-aurantii, is a significant post-harvest disease in citrus, resulting economic losses due to the lack of effective fungicides. This study investigates antifungal activity essential oils from Late IAC 585 willowleaf and Pera sweet orange controlling sour rot Tahiti acid lime fruits. Essential were extracted via hydrodistillation tested vitro vivo. In vitro, assays evaluated mycelial growth inhibition at concentrations ranging 2 32 µl...