Ricardo Harakava

ORCID: 0000-0003-1431-2665
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About
Contact & Profiles
Research Areas
  • Plant Virus Research Studies
  • Plant Pathogens and Fungal Diseases
  • Plant tissue culture and regeneration
  • Plant Pathogenic Bacteria Studies
  • Plant-Microbe Interactions and Immunity
  • Insect-Plant Interactions and Control
  • Phytoplasmas and Hemiptera pathogens
  • Cocoa and Sweet Potato Agronomy
  • Plant and Fungal Interactions Research
  • Banana Cultivation and Research
  • Nematode management and characterization studies
  • Plant Disease Resistance and Genetics
  • Entomopathogenic Microorganisms in Pest Control
  • Mycorrhizal Fungi and Plant Interactions
  • Yeasts and Rust Fungi Studies
  • Transgenic Plants and Applications
  • Insect Resistance and Genetics
  • Plant Parasitism and Resistance
  • Plant Disease Management Techniques
  • Legume Nitrogen Fixing Symbiosis
  • Plant and animal studies
  • Insect symbiosis and bacterial influences
  • Mycotoxins in Agriculture and Food
  • Insect and Arachnid Ecology and Behavior
  • Insect Pest Control Strategies

Instituto Biológico
2016-2025

Universidade de São Paulo
2004-2023

Agência Paulista de Tecnologia dos Agronegócios
2018-2023

Secretaria de Agricultura e Abastecimento
2014-2022

Cornell University
2022

US Biologic (United States)
2020

United States Department of State
2017-2019

University of Florida
2003-2018

Brazilian Agricultural Research Corporation
2017-2018

Methodist University of Piracicaba
2014

Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing interrogans serovar Copenhageni comparative analysis with the available Lai reveal that despite overall genetic similarity there are structural differences, large chromosomal inversion extensive variation number distribution insertion sequence elements. Genome elucidates many novel aspects leptospiral...

10.1128/jb.186.7.2164-2172.2004 article EN Journal of Bacteriology 2004-03-17

Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...

10.1128/jb.185.3.1018-1026.2003 article EN Journal of Bacteriology 2003-01-18

Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...

10.1590/s1677-04202006000100008 article EN Brazilian Journal of Plant Physiology 2006-03-01

The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. single circular chromosome CTCB07 2.6 Mb in length with a GC content 68% 2,044 predicted open reading frames. analysis also revealed 307 pseudogenes, is more than any bacterial plant pathogen sequenced to date. Many these if functional, would likely be involved the degradation heteropolysaccharides, uptake free sugars, synthesis amino acids. Although L. has...

10.1094/mpmi.2004.17.8.827 article EN other-oa Molecular Plant-Microbe Interactions 2004-08-01

Liberibacter spp. form a Rhizobiaceae clade of phloem-limited pathogens limited host range. Two obligately parasitic species have been sequenced: 'Candidatus asiaticus', which causes citrus huanglongbing (HLB) worldwide, and 'Ca. L. solanacearum', potato "zebra chip" disease. A third (proposed) species, crescens, was isolated from mountain papaya, grown in axenic culture, sequenced. In an effort to identify common determinants, the complete genomic DNA sequence second HLB americanus' strain...

10.1094/mpmi-09-13-0292-r article EN other-oa Molecular Plant-Microbe Interactions 2013-11-07

Citrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, fruit causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during survey noncommercial areas southeast region Brazil 2013 2016. Transmission electron microscopy analyses foliar confirmed presence rod-like particles commonly associated with nucleus cytoplasm infected...

10.1094/phyto-02-17-0042-r article EN other-oa Phytopathology 2017-04-11

Abstract The fungus, Sclerotinia sclerotiorum, causes white mold disease and infects a broad spectrum of host plants (> 500), including soybean with yield losses up to 70%. Biological control is potential alternative for management this severe plant pathogen, relative chemical fungicides, provides benefits the environment, farmers consumers. symbiotic bacteria entomopathogenic nematodes, Xenorhabdus spp. Photorhabdus spp., are characterized by production antimicrobial compounds, which...

10.1038/s41598-020-77472-6 article EN cc-by Scientific Reports 2020-11-26

In South America, Solenopsis saevissima and S. invicta are the most common fire ants. Nests founded in areas under anthropic interference like urban or rural areas, but is found preferentially those with greatest interference. However, we do not know rates at which they exist anthropized next to high density of native vegetation. Areas 60 90% Atlantic Forest were selected verify occurrence both species areas. We investigated molecular diversity applied reconstruction ancestral state analysis...

10.1590/1678-4685-gmb-2021-0120 article EN cc-by Genetics and Molecular Biology 2022-01-01

Abstract: The objective of this work was to evaluate, in vitro and vivo, the potential Trichoderma spp. strains control Sclerotinia sclerotiorum soybeans (Glycine max) perform molecular identification best perfoming strains. effect 120 on viability S. sclerotia evaluated through immersion suspension conidia from antagonists plating culture medium. performing were a greenhouse, for pathogen inoculated 'Pintado' soybean seeds plants. Of tested vitro, 22 caused 100% inhibition germination. In...

10.1590/s0100-204x2017001200002 article EN cc-by Pesquisa Agropecuária Brasileira 2017-12-01

Local chlorotic spots resembling early lesions characteristic of citrus leprosis (CL) were observed in leaves two sweet orange (Citrus sinensis L.) trees Teresina, State Piauí, Brazil, 2017. However, despite the similarities, these generally larger than those a typical CL and showed rare or no necrosis symptoms. In symptomatic tissues, transmission electron microscopy revealed presence viroplasms nuclei infected parenchymal cells rod-shaped particles with an average size approximately 40 ×...

10.1094/pdis-09-17-1425-re article EN other-oa Plant Disease 2018-02-27

Passion fruit green spot and passion sudden death are two reportedly distinct viral diseases that recurrently affect (Passiflora spp.) groves in Brazil. Here we used a systematic approach interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, high-throughput sequencing of the RNA affected plants to gain insights about these diseases. Our data confirmed not only involvement cileviruses pathologies, as previously suggested, but also...

10.3389/fmicb.2020.00206 article EN cc-by Frontiers in Microbiology 2020-02-14

Eucalyptus ESTs libraries were screened for genes involved in lignin biosynthesis. This search was performed under the perspective of recent revisions on monolignols biosynthetic pathway. orthologues all phenylpropanoid pathway leading to biosynthesis reported other plant species identified. A library made with mRNAs extracted from wood enriched and allowed infer isoforms each gene family that play a major role formation. Analysis suggests that, besides enzymes phenylpropanoids pathway,...

10.1590/s1415-47572005000400015 article EN cc-by Genetics and Molecular Biology 2005-01-01

Citrus black spot, caused by Guignardia citricarpa, is a serious fruit spot disease and widely distributed in Asia, southern Africa, South America, but does not occur North America or the Mediterranean region. A nonpathogenic species, G. mangiferae, cosmopolitan with wide host range can colonize citrus leaves saprophytically. Detection identification of spp. on necessary for epidemiological, management, regulatory purposes. In this study, we compared published unpublished polymerase chain...

10.1094/pdis-91-5-0525 article EN other-oa Plant Disease 2007-05-01

Despite the importance of viral strains/variants as agents emerging diseases, genetic and evolutionary processes affecting their ecology are not fully understood. To get insight into this topic, we assessed population spatial dynamic parameters citrus leprosis virus C (CiLV-C, genus Cilevirus , family Kitaviridae ). CiLV-C is etiological agent disease, a non-systemic infection considered main disorder orchards in Brazil. Overall, obtained 18 complete or near-complete genomes, 123 nucleotide...

10.3389/fmicb.2021.641252 article EN cc-by Frontiers in Microbiology 2021-04-29
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