A5046142910- Leptospirosis research and findings
- Veterinary medicine and infectious diseases
- Viral Infections and Vectors
- bioluminescence and chemiluminescence research
- Parasites and Host Interactions
- Yersinia bacterium, plague, ectoparasites research
- Vector-borne infectious diseases
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Iron Metabolism and Disorders
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Trace Elements in Health
- Redox biology and oxidative stress
- Genomics and Phylogenetic Studies
- Viral Infections and Immunology Research
- Tuberculosis Research and Epidemiology
- Nicotinic Acetylcholine Receptors Study
- Free Radicals and Antioxidants
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Toxin Mechanisms and Immunotoxins
Instituto Butantan
2016-2025
Universidade de São Paulo
2013-2025
Instituto Adolfo Lutz
2008
European Bioinformatics Institute
2007
Wellcome Trust
2007
Broad Institute
2007
Virginia Tech
2007
Johns Hopkins University
2007
Universidade Estadual de Campinas (UNICAMP)
2000-2001
Universidade Federal de São Paulo
2000-2001
We present a draft sequence of the genome Aedes aegypti, primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times size malaria Anopheles gambiae. Nearly 50% Ae. aegypti consists transposable elements. These contribute to factor 4 6 increase in average gene length sizes intergenic regions relative An. gambiae Drosophila melanogaster. Nonetheless, chromosomal synteny generally maintained among all three insects, although conservation...
Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing interrogans serovar Copenhageni comparative analysis with the available Lai reveal that despite overall genetic similarity there are structural differences, large chromosomal inversion extensive variation number distribution insertion sequence elements. Genome elucidates many novel aspects leptospiral...
We report here the construction of a vector derived from pET3-His and pRSET plasmids for expression purification recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined advantages both vectors: small size (pRSET), short 6XHis tag at N-terminus (pET3-His) high copy number (pRSET). (2.8 kb) number/cell (200-250 copies) facilitate subcloning sequencing procedures when compared to pET system (pET3-His, 4.6 kb 40-50 also result level (20 mg...
Leptospirosis, caused by spirochetes of the genus Leptospira, is a globally widespread, neglected and emerging zoonotic disease. While whole genome analysis individual pathogenic, intermediately pathogenic saprophytic Leptospira species has been reported, comprehensive cross-species genomic comparison all known infectious non-infectious with goal identifying genes related to pathogenesis mammalian host adaptation, remains key gap in field. Infectious comprised evolutionarily diverged from...
We report novel features of the genome sequence Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. species colonize significant proportion rodent populations worldwide and produce life-threatening infections in mammals. Genomic analysis reveals presence competent transport system with 13 families genes encoding for major transporters including three-member component efflux compatible long-term survival this organism. The leptospiral contains broad array regulatory...
Pathogenic leptospires have the ability to survive and disseminate multiple organs after penetrating host. Several pathogens, including spirochetes, been shown express surface proteins that interact with extracellular matrix (ECM). This adhesin-mediated binding process seems be a crucial step in colonization of host tissues. study examined interaction putative leptospiral outer membrane laminin, collagen type I, IV, cellular fibronectin, plasma fibronectin. Six predicted coding sequences...
Abstract Background It has been well documented over past decades that interaction of pathogens with the extracellular matrix (ECM) plays a primary role in host cell attachment and invasion. Adherence to tissues is mediated by surface-exposed proteins expressed microorganisms during infection. The mechanisms which pathogenic leptospires invade colonize remain poorly understood since few virulence factors contributing pathogenesis disease have identified. Whole-genome sequencing analysis L....
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
Leptospirosis is an important global human and veterinary health problem. Humans can be infected by exposure to chronically animals their environment. An focus of the current leptospiral research identification outer membrane proteins (OMPs). Due location, OMPs are likely relevant in host–pathogen interactions, hence potential ability stimulate heterologous immunity. The existing whole-genome sequence Leptospira interrogans serovar Copenhageni offers a unique opportunity search for cell...
Background Leptospirosis is a multisystem disease caused by pathogenic strains of the genus Leptospira. We have reported that Leptospira are able to bind plasminogen (PLG), generate active plasmin in presence activator, and degrade purified extracellular matrix fibronectin. Methodology/Principal Findings now cloned, expressed 14 leptospiral recombinant proteins. The proteins were confirmed be surface exposed immunofluorescence microscopy evaluated for their ability (PLG). identified eight as...
ABSTRACT Leptospirosis is a zoonosis with multisystem involvement caused by pathogenic strains of the genus Leptospira . OmpL1 an outer membrane protein spp. that expressed during infection. In this work, we investigated novel features protein. We describe leptospiral extracellular matrix (ECM)-binding and plasminogen (PLG) receptor. The recombinant was in Escherichia coli BL21(DE3) Star/pLysS as inclusion bodies, refolded, purified metal-chelating chromatography. presented typical β-strand...
Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease human and veterinary concern. The identification novel proteins that mediate host-pathogen interactions important for understanding bacterial pathogenesis as well to identify protective antigens would help fight disease. We describe in this work cloning, expression, purification characterization three predicted leptospiral membrane proteins, LIC10258, LIC12880 (Lp30) LIC12238. have employed Escherichia coli...
Abstract Background Leptospirosis is considered a re-emerging infectious disease caused by pathogenic spirochaetes of the genus Leptospira . Pathogenic leptospires have ability to survive and disseminate multiple organs after penetrating host. Leptospires were shown express surface proteins that interact with extracellular matrix (ECM) plasminogen (PLG). This study examined interaction two putative leptospiral laminin, collagen Type I, IV, cellular fibronectin, plasma PLG, factor H C4bp....
Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Surface proteins have potential to promote several activities, including adhesion. This work aimed study leptospiral coding sequence (CDS) LIC11087, genome annotated as hypothetical outer membrane protein. The LIC11087 gene was cloned and expressed in Escherichia coli BL21 (DE3) strain by using expression vector pAE. recombinant protein tagged with N-terminal 6XHis...
ABSTRACT Pathogenic Leptospira species are the etiological agents of leptospirosis, a widespread disease human and veterinary concern. In this study, we report that capable binding plasminogen (PLG) in vitro. The to leptospiral surface was demonstrated by indirect immunofluorescence confocal microscopy with living bacteria. PLG bacteria seems occur via lysine residues because ligation is inhibited addition analog 6-aminocaproic acid. Exogenously provided urokinase-type activator (uPA)...
Leptospirosis is a widespread re-emerging zoonosis of human and veterinary concern. It has been shown that virulent leptospires protect themselves against the host's innate immune system, strategy allows bacteria to reach immunologically safe environments. Although extensive studies on host-pathogen interactions have performed, little known how deal with host attack. In previous work, we demonstrated ability bind plasminogen (PLG), after treatment activators, conferred plasmin (PLA) activity...
The regulation of gene expression by environmental signals, such as temperature and osmolarity, has been correlated with virulence. In this study, we characterize the protein LipL53 from Leptospira interrogans, previously shown to react serum sample individual diagnosed leptospirosis be up-regulated shift physiological osmolarity. recombinant was expressed in Escherichia coli system, insoluble form, recovered urea solubilization further refolded decreasing denaturing agent concentration...
Leptospirosis is an emerging infectious disease caused by pathogenic species of Leptospira. In this work, we report the cloning, expression, purification, and characterization two predicted leptospiral outer membrane proteins, LIC11469 LIC11030. The protein well conserved among strains, while LIC11030 was identified only in Leptospira interrogans. We confirmed surface proteolysis intact leptospires with proteinase K that these proteins are most likely new proteins. recombinant were evaluated...