A5045451934- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Platelet Disorders and Treatments
- Helicobacter pylori-related gastroenterology studies
- Viral-associated cancers and disorders
- Cancer-related gene regulation
- HIV-related health complications and treatments
- Gastric Cancer Management and Outcomes
- Blood groups and transfusion
- Molecular Biology Techniques and Applications
- Systemic Lupus Erythematosus Research
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
- Myasthenia Gravis and Thymoma
- Pediatric health and respiratory diseases
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Gastrointestinal disorders and treatments
- CRISPR and Genetic Engineering
- HIV/AIDS oral health manifestations
- T-cell and Retrovirus Studies
Universidade Estadual Paulista (Unesp)
2013-2024
Medecell (Brazil)
2024
Biotech (Brazil)
2024
Hospital São Paulo
2021
Universidade Tecnológica Federal do Paraná
2016
Universidade de São Paulo
2000-2011
Universidade Federal do Ceará
2009
Universidade Estadual de Campinas (UNICAMP)
2000-2001
Universidade Federal de São Paulo
2000-2001
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
Detection and quantification of hepatitis C virus (HCV) RNA is integral to diagnostic therapeutic regimens. All molecular assays target the viral 5'-noncoding region (5'-NCR), all show genotype-dependent variation sensitivities load results. Non-western HCV genotypes have been under-represented in evaluation studies. An alternative within genome could facilitate a new generation assays.In this study we determined by de novo sequencing that 3'-X-tail element, characterized significantly later...
Transcribed sequences in the human genome can be identified with confidence only by alignment derived from cDNAs synthesized naturally occurring mRNAs. We constructed a set of 250,000 that represent partial expressed gene and are biased toward central coding regions resulting transcripts. They termed ORF sequence tags (ORESTES). The ORESTES were assembled into 81,429 contigs. Of these, 1,181 (1.45%) found to match chromosome 22 at least one contig for 162 (65.6%) 247 known genes, 67 (44.6%)...
We aimed to evaluate the inactivation of COX-2, HMLH1 and CDKN2A by promoter methylation its relationship with infection different Helicobacter pylori strains in gastric cancer.DNA extracted from 76 H. pylori-positive tumor samples was available for identification methylation-specific PCR subtyping PCR. Immunohistochemistry used determine p16(INK4A) expression.A strong negative correlation found between expression these markers presence their genes. Among cardia tumors, negativity a...
Helicobacter pylori infection is an established risk factor for gastric cancer development, but the exact underlying mechanism still remains obscure. The inactivation of tumor suppressor genes such as p53 and p27(KIP1) a hypothesized mechanism, although there no consensus regarding influence H. cagA(+) in development these genetic alterations.To verify relationship among infection, mutations p27(Kip1) Protein (p27) expression adenocarcinomas (GA) seventy-four tissues were assayed by PCR cagA...
The goal of treatment chronic hepatitis C is to achieve a sustained virological response, which defined as exhibiting undetectable virus (HCV) RNA levels in serum following therapy for at least six months. However, the current only effective 50% patients infected with HCV genotype 1, most prevalent Brazil. Inhibitors serine protease non-structural protein 3 (NS3) have therefore been developed improve responses HCV-infected patients. emergence drug-resistant variants has major obstacle...
In Brazil, the Amazon Basin is endemic for hepatitis D virus (HDV) infection; however, studies in other regions of country are scarce. This study aims to map seroepidemiological situation anti-Delta antibodies chronic B carriers all five Brazilian geographic regions. Serum samples from 1240 HBsAg positive individuals (55.4% men; mean age 43.1 ± 13.4 years) 24 26 states were tested presence using a commercial immunoassay. Anti-Delta detected 40 (3.2%; 52.5% female; 38.1 13.8 years). Age less...
Although HCV has hepatic tropism, the presence of virus in extra-hepatic compartments been well documented. Platelets have described as carriers circulation and may be a natural reservoir for virus. However, few studies performed to evaluate levels RNA plasma platelets are equal or differ some way. Therefore, aim this study was perform comparative evaluation stability isolated platelets. Four aliquots whole obtained from patients infected with were incubated at 37 °C 0, 48, 96 144 h. After...
Gastric cancer is one of the top list types that most leads to death in Brazil and worldwide. Helicobacter pylori(H. pylori) a class I carcinogen infect almost 90% chronic gastritis patients. Some genotypes confer different virulent potential H. pylori can increase risk development. Methylation CpG islands inactivate tumor suppressor genes therefore, it be involved tumorigenic process. CDH1 gene encodes E-cadherin protein, which important maintaining cell-cell contacts. The inactivation this...
Promoter hypermethylation of CDKN2A (p16INK4A protein) is the main mechanism gene inactivation. However, its association with Helicobacter pylori infection a controversial issue. Therefore, we examined series gastric adenocarcinomas to assess between p16INK4A inactivation and H. genotype (vacA, cagA, cagE, virB11 flaA) according location histological subtype tumors. expression promoter methylation were found in 77 adenocarcinoma samples by immunohistochemistry methylation-specific PCR,...
Abstract Studies have suggested that hepatitis C virus (HCV) may infect not only hepatocytes but also be carried by platelets. Platelets express more than 20 polymorphic antigenic determinants on their surface, which are called human platelet antigens (HPA). To determine the allele frequency of HPA‐1 to ‐5 in patients infected with HCV, blood samples were collected from 257 donors for control group and 191 HCV. DNA was isolated amplified genes ‐4 using PCR Sequence Specific Primers (PCR‐SSP)...
Abstract This study contributes to the understanding of mechanisms associated with signs and symptoms tooth eruption, by investigating presence mast cells in pericoronal tissues during intraosseous (Group 1) submucosal 2) phases eruption. We compared findings for these two groups each other those oral mucosa 3). In group, 14 specimens were analyzed microscopically after hematoxylin eosin staining immunohistochemical analysis c-Kit tryptase expression. Results revealed that number density is...
Abstract Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death in world, and about 80% cases are associated with hepatitis B or C. Genetic epigenetic alterations accumulated over decades chronic injury may affect functioning tumor suppressor genes protooncogenes. Studies have evidenced role Long non-coding RNAs (LncRNA) oncogenic activities, suggesting a great potential treatment, diagnosis indicator prognosis cancer. In this context, aim study was to evaluate...
INTRODUÇÃO: Apesar da alta freqüência de infecção por Helicobacter pylori na população, somente uma minoria indivíduos desenvolve câncer gástrico. É provável que a colonização mucosa cepas patogênicas, levando maior agressão e inflamação seja um dos elos cadeia eventos oncogênese gástrica. OBJETIVOS: Investigar patogênicas cagA vacA do H. em pacientes com MATERIAL E MÉTODOS: Foram estudados retrospectivamente 42 A foi avaliada exame histológico pelo PCR para identificação genótipos amostras...
Epstein-Barr virus (EBV) has been associated with 10% of gastric carcinomas. The aim this study was to determine the frequency EBV in carcinomas Brazil assessed by situ hybridization (ISH) and PCR, which would contribute characterization clinical pathological aspects EBV-associated One hundred ninety-two carcinoma cases were collected at hospitals two Brazilian states. Seventy-three out 151 PCR(+), while 11/160 ISH(+). Nine eleven ISH(+) displayed a diffuse staining pattern 2 11 focal...
Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have described as carriers circulation infected patients. Platelets do not express cluster differentiation 81 CD81, main receptor for hepatocytes, although this protein found megakaryocytes. Still, it is clear if HCV interacts with platelets directly or interaction a consequence its association The aim study was to evaluate from...
Although progression of fibrosis in the chronic hepatitis C depends on environmental, viral, and host factors, genetic polymorphisms have been associated recently with this progression, including expression integrins, adhesion proteins. Some integrins expressed platelet membrane show polymorphic antigenic determinants called human antigens (HPA), where major ones are HPA-1, -3, -5. The association between HCV infection HPA-5b has demonstrated. Similarly, HPA profile could determine if is...
The objective of this study was to investigate human leucocyte antigen (HLA) genes in patients chronically infected with hepatitis C virus (HCV) and analyse the possible role these progression chronic C. One hundred forty-five (145) Brazilian only HCV genotype 1 were evaluated. HLA class I (A, B, C) II (DRB1, DQA1, DQB1) typing carried out by PCR-SSO, through Luminex technology. Associations found protection against development liver damage both DRB1 11 (5.0% versus 18.2%, P=0.0016, OR=0.23,...
The combination of pegylated interferon (PEG-INF) and ribavirin is currently the best treatment for chronic hepatitis C, providing a sustained virological response (SVR) in 54%-63% patients. In patients infected with C virus (HCV) genotype 1, SVR rate 42%-52%. To evaluate efficacy this drug combination, we conducted an open, prospective study 58 consecutive treatment-naïve HCV 1 treated at university hospital, comparing those presenting (SVRs), nonresponders (NRs), relapsers (RELs). Among...
INTRODUCTION: Transforming growth factor beta 1 (TGFB1) and platelet-derived (PDGF) are the main cytokines related to hepatic fibrogenesis. METHODS: RNA isolated from platelets tissue of 43 HCV carriers was used for quantitative polymerase chain reaction determine TGFB1, PDGFA, PDGFB expression. RESULTS: The mRNA expression PDGFA in significantly lower group with advanced fibrosis than early-stage fibrosis. TGFB1 more frequently expressed tissue, which different PDGFB. CONCLUSIONS: A pathway...
Hepatitis B virus (HBV) is an enveloped that induces chronic liver disease. HBV has been classified into eight genotypes (A–H) according to its genome sequence by using Sanger sequencing or reverse hybridization. often restricted analyzing the S gene and inaccurate for detecting minority genetic variants, whereas hybridization detects only known mutations. Next-generation (NGS) a robust tool clinical virology with different protocols available. The objective of this study was develop new...