Yongmei Zhao
A5041061250- Cancer Genomics and Diagnostics
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Genomics and Rare Diseases
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Birth, Development, and Health
- RNA regulation and disease
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Pregnancy and preeclampsia studies
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Renal Diseases and Glomerulopathies
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
- Renal and related cancers
- HIV Research and Treatment
- CRISPR and Genetic Engineering
Frederick National Laboratory for Cancer Research
2015-2025
National Cancer Institute
2015-2025
National Institutes of Health
2017-2024
Center for Cancer Research
2017-2024
Leidos (United States)
2015-2024
Leidos Biomedical Research Inc. (United States)
2018-2024
Tohoku University Hospital
2024
Georgetown University
2024
University of Florida
2024
University at Buffalo, State University of New York
2024
Diffuse large B-cell lymphomas (DLBCLs) are phenotypically and genetically heterogeneous. Gene-expression profiling has identified subgroups of DLBCL (activated B-cell–like [ABC], germinal-center [GCB], unclassified) according to cell origin that associated with a differential response chemotherapy targeted agents. We sought extend these findings by identifying genetic subtypes based on shared genomic abnormalities uncover therapeutic vulnerabilities tumor genetics.
We present a draft sequence of the genome Aedes aegypti, primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times size malaria Anopheles gambiae. Nearly 50% Ae. aegypti consists transposable elements. These contribute to factor 4 6 increase in average gene length sizes intergenic regions relative An. gambiae Drosophila melanogaster. Nonetheless, chromosomal synteny generally maintained among all three insects, although conservation...
Cellular diversity in tumors is a key factor for therapeutic failures and lethal outcomes of solid malignancies. Here, we determined the single-cell transcriptomic landscape liver cancer biospecimens from 19 patients. We found varying degrees heterogeneity malignant cells within between diverse landscapes tumor microenvironment (TME). Strikingly, with higher were associated patient's worse overall survival. link hypoxia-dependent vascular endothelial growth expression TME polarization....
Intratumor molecular heterogeneity of hepatocellular carcinoma is partly attributed to the presence hepatic cancer stem cells (CSCs). Different CSC populations defined by various cell surface markers may contain different oncogenic drivers, posing a challenge in defining molecularly targeted therapeutics. We combined transcriptomic and functional analyses at single‐cell level assess degree heterogeneity. provide evidence that CSCs are phenotypically, functionally, transcriptomically...
Solid tumors elicit a detectable immune response including the infiltration of tumor-associated macrophages (TAMs). Unfortunately, this is co-opted into contributing toward tumor growth instead preventing its progression. We seek to reestablish an antitumor by selectively targeting surface receptors and endogenous signaling processes macrophage subtypes driving cancer RP-182 synthetic 10-mer amphipathic analog host defense peptides that induces conformational switch mannose receptor CD206...
Small-cell lung cancer (SCLC) is an aggressive neuroendocrine cancer. Oncogenic MYC amplifications drive SCLC heterogeneity, but the genetic mechanisms of amplification and phenotypic plasticity, characterized by nonneuroendocrine cell states, are not known. Here, we integrate whole-genome sequencing, long-range optical mapping, single-cell DNA fluorescence in situ hybridization to find extrachromosomal (ecDNA) as a primary source oncogene driver fusions. ecDNAs bring proximity enhancer...
Astrocytes are coupled via gap junctions (GJs) comprising connexin 43 (Cx43) (<i>Gja1</i>) and Cx30 (<i>Gjb6</i>), which facilitate intercellular exchange of ions. Astrocyte connexins also form heterotypic GJs with oligodendrocytic somata lamellae. Loss oligodendrocyte results in myelin pathology. However, whether loss astrocyte affects oligodendrocytes is not known. To address this question, mice astrocyte-targeted deletion Cx43 global [double knock-out (dKO)] were studied using Western...
Abstract Extracellular nucleotide di‐ and triphosphates such as ATP ADP mediate their effects through purinergic P2 receptors belonging to either the metabotropic P2Y or ionotropic P2X receptor family. The 7 R is a unique member of family, which forms pore in response ligand stimulation, regulating cell permeability, cytokine release, and/or apoptosis. This also that its affinity for benzoyl‐benzoyl (BzATP) at least 10‐fold greater than ATP. Primary human fetal astrocytes culture express...
Activation of the unfolded protein response (UPR) is involved in pathogenesis numerous CNS myelin abnormalities; yet, its direct role traumatic spinal cord injury (SCI)-induced demyelination not known. The UPR an evolutionarily conserved cell defense mechanism initiated to restore endoplasmic reticulum homeostasis various cellular stresses including infection, trauma, and oxidative damage. However, if uncompensated, triggers apoptotic death. We demonstrate that three signaling branches PERK,...
Abstract Although histone H3 lysine 27 trimethylation (H3K27Me3) is associated with gene silencing, whether H3K27Me3 demethylation affects transcription and cell differentiation in vivo has remained elusive. To investigate this, we conditionally inactivated the two demethylases, Jmjd3 Utx, non-dividing intrathymic CD4 + T-cell precursors. Here show that both enzymes redundantly promote removal at, expression of, a specific subset of genes involved terminal thymocyte differentiation,...
Objective Hepatocellular carcinoma (HCC) represents a typical inflammation-associated cancer. Tissue resident innate lymphoid cells (ILCs) have been suggested to control tumour surveillance. Here, we studied how the local cytokine milieu controls ILCs in HCC. Design We performed bulk RNA sequencing of HCC tissue as well flow cytometry and single-cell enriched from non-tumour liver, margin core derived 48 patients with Simultaneous measurement protein expression at level (AbSeq) identified...
Abstract Most current studies rely on short-read sequencing to detect somatic structural variation (SV) in cancer genomes. Long-read offers the advantage of better mappability and long-range phasing, which results substantial improvements germline SV detection. However, long-read detection methods do not generalize well analysis SVs tumor genomes with complex rearrangements, heterogeneity, aneuploidy. Here, we present Severus: a method for accurate different types using phased breakpoint...
TLR3 functions as a viral nucleic acid sentinel activated by dsRNA viruses and virus replication intermediates within intracellular vesicles. To explore the spectrum of genes induced in human astrocytes TLR3, we used microarray approach analog polyriboinosinic polyribocytidylic (pIC) ligand. As expected for TLR activation, pIC wide array cytokines chemokines known their role inflammatory responses, well up-regulation receptor itself. The data also showed activation broad antiviral response...
Ischemic stroke is a typical cerebrovascular illness with high morbidity and mortality worldwide. Nevertheless, strategies for the prevention treatment of cerebral ischemia/reperfusion injury (CIRI) are limited. Gallic acid (GA) plant polyphenol that has been used against CIRI. However, pharmacokinetic (PK) properties GA, such as its low absorption, poor bioavailability, quick elimination, have negative effects on application. To strengthen effectiveness, delivery system GA-loaded...
IntroductionIndividuals with focal segmental glomerular sclerosis (FSGS) typically undergo kidney biopsy only once, which limits the ability to characterize cell gene expression over time.MethodsWe used single-cell RNA sequencing (scRNA-seq) explore disease-related molecular signatures in urine cells from subjects FSGS. We collected 17 samples 12 FSGS and captured these as 23 samples. The inflammatory renal epithelial immune were evaluated bulk data sets of minimal change disease (MCD) (The...
Abstract The BCL2-inhibitor, Venetoclax (VEN), has shown significant anti-leukemic efficacy in combination with the DNMT-inhibitor, Azacytidine (AZA). To explore mechanisms underlying selective sensitivity of mutant leukemia cells to VEN and AZA, we used cell-based isogenic models containing a common leukemia-associated mutation epigenetic regulator ASXL1 . KBM5 CRISPR/Cas9-mediated correction G710X showed reduced leukemic growth, increased myeloid differentiation, decreased HOXA BCL2 gene...