A5079194924- Chemokine receptors and signaling
- Cancer, Lipids, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Calcium signaling and nucleotide metabolism
- Immune cells in cancer
- Lysosomal Storage Disorders Research
- Ion Channels and Receptors
- Immunotherapy and Immune Responses
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Brain Metastases and Treatment
- Advanced Memory and Neural Computing
- Peptidase Inhibition and Analysis
- Lung Cancer Research Studies
- Conducting polymers and applications
- Andrographolide Research and Applications
- Carbohydrate Chemistry and Synthesis
- Hemoglobinopathies and Related Disorders
- Nanoplatforms for cancer theranostics
- Piperaceae Chemical and Biological Studies
- Cellular transport and secretion
- SARS-CoV-2 and COVID-19 Research
- HER2/EGFR in Cancer Research
- Plant Molecular Biology Research
- Blood groups and transfusion
National Center for Advancing Translational Sciences
2016-2025
National Institutes of Health
1965-2024
National Institutes of Health Clinical Center
1965
Mount Sinai Hospital
1965
The Mount
1965
Mount Sinai Hospital
1965
Abstract Cellular stresses trigger autophagy to remove damaged macromolecules and organelles. Lysosomes ‘host’ multiple stress-sensing mechanisms that the coordinated biogenesis of autophagosomes lysosomes. For example, transcription factor (TF)EB, which regulates lysosome biogenesis, is activated following inhibition mTOR, a lysosome-localized nutrient sensor. Here we show reactive oxygen species (ROS) activate TFEB via lysosomal Ca 2+ -dependent mechanism independent mTOR. Exogenous...
Solid tumors elicit a detectable immune response including the infiltration of tumor-associated macrophages (TAMs). Unfortunately, this is co-opted into contributing toward tumor growth instead preventing its progression. We seek to reestablish an antitumor by selectively targeting surface receptors and endogenous signaling processes macrophage subtypes driving cancer RP-182 synthetic 10-mer amphipathic analog host defense peptides that induces conformational switch mannose receptor CD206...
A lysosome activator ameliorates muscular dystrophies.
Glucocerebrosidase (GCase) is implicated in both a rare, monogenic disorder (Gaucher disease, GD) and common, multifactorial condition (Parkinson’s PD); hence, it an urgent therapeutic target. To identify correctors of severe protein misfolding trafficking obstruction manifested by the pathogenic L444P-variant GCase, we developed suite quantitative, high-throughput, cell-based assays. First, labeled GCase with small proluminescent HiBiT peptide reporter tag, enabling quantitation...
Mammalian two-pore-channels (TPC1, 2; TPCN1, TPCN2) are ubiquitously- expressed, PI(3,5)P2-activated, Na+-selective channels in the endosomes and lysosomes that regulate luminal pH homeostasis, membrane trafficking, Ebola viral infection. Whereas channel activity of TPC1 is strongly dependent on voltage, TPC2 lacks such voltage dependence despite presence presumed 'S4 voltage-sensing' domains. By performing high-throughput screening followed by lysosomal electrophysiology, here we identified...
Abstract Brain metastasis occurs in about 50% of all women with metastatic HER2+ breast cancer and confers poor prognosis for patients. Despite effective HER2-targeted treatments peripheral trastuzumab HER2 inhibitors, limited brain permeability renders these inefficient (BCBM). The scarcity suitable patient-derived vivo models BCBM has curtailed the study molecular mechanisms that promote growth therapeutic resistance metastasis. Here, we generated characterized a luminal B cell model...
<p>Effect of ErbB inhibitors on cell viability in BCBM94 and HME-1 cells</p>
<p>Comparison ErbB inhibitor response in BCBM94 and NGN2 neurons</p>
<p>ErbB inhibitor response in the presence of rhNRG1</p>
<p>Supplemental figures and legends</p>
<div>Abstract<p>Brain metastasis occurs in about 50% of all women with metastatic HER2<sup>+</sup> breast cancer and confers poor prognosis for patients. Despite effective HER2-targeted treatments peripheral trastuzumab HER2 inhibitors, limited brain permeability renders these inefficient metastasis. The scarcity suitable patient-derived <i>in vivo</i> models has curtailed the study molecular mechanisms that promote growth therapeutic resistance In this...
ABSTRACT Brain metastasis of HER2+ breast cancer occurs in about 50% all women with metastatic and confers poor prognosis for patients. Despite effective HER2-targeted treatments peripheral Trastuzumab +/-HER2 inhibitors, limited brain permeability renders these inefficient (BCBM). The scarcity suitable patient-derived in-vivo models BCBM has compromised the study molecular mechanisms that promote growth therapeutic resistance metastasis. We have generated characterized new cells (BCBM94)...
Compare the treatment efficacy of a derivative Docosahexaenoic acid (DHA), Synaptamide (SYN) versus its analog, Dimethylsynaptamide (DMS) in suppression experimental allergic encephalomyelitis (EAE) mice.
As tumor-associated macrophages (TAMs) exercise a plethora of pro-tumor and immune evasive functions, novel strategies targeting TAMs to inhibit tumor progression have emerged within the current arena cancer immunotherapy. Activation mannose receptor 1 (Mrc1; CD206) is recent approach that recognizes suppressive CD206high M2-like as drug target. Ligation CD206 both induces reprogramming towards pro-inflammatory phenotype selectively triggers apoptosis in these cells. CD206-activating...
<p>Suppl Fig 7</p>
<p>Suppl Fig 5</p>
<p>Suppl Fig 4</p>
<p>Suppl Fig 2</p>
<div>Abstract<p>As tumor-associated macrophages (TAM) exercise a plethora of protumor and immune evasive functions, novel strategies targeting TAMs to inhibit tumor progression have emerged within the current arena cancer immunotherapy. Activation mannose receptor 1 (CD206) is recent approach that recognizes immunosuppressive CD206<sup>high</sup> M2-like as drug target. Ligation CD206 both induces reprogramming toward proinflammatory phenotype selectively triggers...