A5008279829- Ion channel regulation and function
- Calcium signaling and nucleotide metabolism
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Cystic Fibrosis Research Advances
- Cardiac electrophysiology and arrhythmias
- Advanced Memory and Neural Computing
- Magnesium in Health and Disease
- Ion Channels and Receptors
- Cellular transport and secretion
- Piperaceae Chemical and Biological Studies
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Neonatal Respiratory Health Research
- Nonlinear Differential Equations Analysis
- Ferroelectric and Piezoelectric Materials
- Transition Metal Oxide Nanomaterials
- Antioxidants, Aging, Portulaca oleracea
- Neuroinflammation and Neurodegeneration Mechanisms
- Recycling and Waste Management Techniques
- Heavy Metal Exposure and Toxicity
- Structural Engineering and Vibration Analysis
- Signaling Pathways in Disease
- GABA and Rice Research
- Sirtuins and Resveratrol in Medicine
Xuzhou Medical College
2018-2025
National Medical Products Administration
2025
Huazhong University of Science and Technology
2024
Tongji Hospital
2024
Nanjing University of Posts and Telecommunications
2016-2023
Liaoning Technical University
2022-2023
China Pharmaceutical University
2022-2023
Yancheng Institute of Technology
2022
Suzhou University of Science and Technology
2022
Jilin University
2019
Significance Lysosomes are the cell’s degradation center. To adapt to different environmental conditions, cell has evolved a set of delicate mechanisms rapidly change lysosome function, which is referred as lysosomal adaptation. Notably, adaptation required for survival under low nutrient conditions. In this study, we identified TRPML1, Ca 2+ -permeant ion channel, an essential player The activity TRPML1 potently (up 10-fold) and increased upon starvation. Furthermore, pharmacological...
Impaired homeostasis of lysosomal Ca2+ causes lysosome dysfunction and storage diseases (LSDs), but the mechanisms by which lysosomes acquire refill are not known. We developed a physiological assay to monitor store refilling using specific activators channels repeatedly induce release. In contrast prevailing view that acidification drives into lysosome, inhibiting V-ATPase H+ pump did prevent refilling. Instead, pharmacological depletion or chelation Endoplasmic Reticulum (ER) prevented...
Lysosomes are best known for their roles in inflammatory responses by engaging autophagy to remove inflammasomes. Here, we describe an unrecognized role the lysosome, showing that it finely controls macrophage function manipulating lysosomal Fe2+—prolyl hydroxylase domain enzymes (PHDs)—NF-κB—interleukin 1 beta (IL1B) transcription pathway directly links lysosomes with responses. TRPML1, a cationic channel, is activated secondarily ROS elevation upon stimuli, which turn suppresses IL1B...
Compelling evidence has demonstrated that macroautophagy/autophagy plays an important role in regulating multiple steps of metastatic cascades; however, the precise autophagy metastasis remains unclear. This study demonstrates inhibition induced by MCOLN1/TRPML1 suppresses cancer evoking ROS-mediated TP53/p53 pathway. First, we found MCOLN1-mediated not only profoundly inhibits both migration and invasion malignant melanoma glioma cell lines vitro, but also vivo. Second, our reveals MCOLN1...
Abstract Objective Dysfunction of calcium/calmodulin (CaM)–dependent kinase II (CaMKII) has been involved in hyperexcitability‐related disorders including epilepsy. However, the relationship between CaMKII and neuronal excitability remains unclear. Methods Neuronal was detected vivo vitro by electroencephalography (EEG), patch clamp multi‐electrode array (MEA), respectively. Next, we assessed currents voltage‐gated sodium channels (VGSCs) clamp, mRNA protein expressions VGSCs were determined...
The resting membrane potential (Δψ) of the cell is negative on cytosolic side and determined primarily by plasma membrane’s selective permeability to K+. We show that lysosomal Δψ set permeabilities Na+ H+, but not K+, positive side. An increase in juxta-lysosomal Ca2+ rapidly reversed activating a large voltage-dependent K+-selective conductance (LysoKVCa). LysoKVCa encoded molecularly SLO1 proteins known for forming BK channels. Opening single channels sufficient cause rapid, striking...
Macroautophagy/autophagy is elevated to ensure the high demand for nutrients growth of cancer cells. Here we demonstrated that MCOLN1/TRPML1 a pharmaceutical target oncogenic autophagy in cancers such as pancreatic cancer, breast gastric malignant melanoma, and glioma. First, showed activating MCOLN1, by increasing expression channel or using MCOLN1 agonists, ML-SA5 MK6-83, arrests autophagic flux perturbing fusion between autophagosomes lysosomes. Second, regulates mediating release zinc...
Different transmembrane (TM) α helices are known to line the pore of cystic fibrosis TM conductance regulator (CFTR) Cl− channel. However, relative alignment these TMs in three-dimensional structure is not known. We have used patch-clamp recording investigate accessibility cytoplasmically applied cysteine-reactive reagents cysteines introduced along length pore-lining first (TM1) a cysteine-less variant CFTR. find that methanethiosulfonate (MTS) irreversibly modify substituted for TM1...
Abstract Both tetrodotoxin‐sensitive (TTX‐S) and TTX‐resistant (TTX‐R) voltage‐dependent Na + channels are expressed in the human neuroblastoma cell line NB‐1, but a gene encoding TTX‐R channel has not been identified. In this study, we have cloned cDNA α subunit of NB‐1 cells designated it hNbR1. The longest open reading frame hNbR1 (accession no. AB158469 ) encodes 2016 amino acid residues. Sequence analysis indicated that is highly homologous with cardiac Nav1.5/ SCN5A > 99% identity....
Lysosomes are best known for their involvement in inflammatory responses, where they participate the macroautophagy/autophagy process to eliminate inflammasomes. Recently, we have identified a previously overlooked function of lysosomes regulating macrophage responses. Specifically, finely control production IL1B (interleukin 1 beta) by manipulating release lysosomal Fe2+ through MCOLN1. Mechanistically, reactive oxygen species (ROS), accumulated during sustained inflammation macrophages,...