A5100367543- CRISPR and Genetic Engineering
- RNA modifications and cancer
- RNA Research and Splicing
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Microbial Natural Products and Biosynthesis
- Carcinogens and Genotoxicity Assessment
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Hemoglobinopathies and Related Disorders
- Cytomegalovirus and herpesvirus research
- Face and Expression Recognition
- Virus-based gene therapy research
Zhejiang University
2008-2025
Chinese Academy of Sciences
2015-2025
China Pharmaceutical University
2010-2025
Shanghai Institute of Organic Chemistry
2020-2025
Chongqing Cancer Hospital
2023-2025
Chongqing University
2023-2025
First Affiliated Hospital Zhejiang University
2022-2024
Shanghai Institute of Materia Medica
2020-2024
Shandong Provincial Hospital
2024
Shandong First Medical University
2024
Abstract Electronic synaptic devices are important building blocks for neuromorphic computational systems that can go beyond the constraints of von Neumann architecture. Although two‐terminal memristive demonstrated to be possible candidates, they suffer from several shortcomings related filament formation mechanism including nonlinear switching, write noise, and high device conductance, all which limit accuracy energy efficiency. Electrochemical three‐terminal transistors, in channel...
Biological synapses store and process information simultaneously by tuning the connection between two neighboring neurons. Such functionality inspires task of hardware implementation neuromorphic computing systems. Ionic/electronic hybrid three-terminal memristive devices, in which channel conductance can be modulated according to history applied voltage current, provide a more promising way emulating substantial reduction complexity energy consumption. 2D van der Waals materials with single...
The ability of animals to respond life-threatening stimuli is essential for survival. Although vision provides one the major sensory inputs detecting threats across animal species, circuitry underlying defensive responses visual remains poorly defined. Here, we investigate innate behaviours elicited by predator-like in mice. Our results demonstrate that neurons superior colliculus (SC) are a variety acute and persistent overhead looming stimuli. Optogenetic mapping revealed SC projections...
Bacteria colonize almost all parts of the human body and can differ significantly. However, population level transcriptomics measurements only describe average bacteria behaviors, ignoring heterogeneity among bacteria. Here, we report a droplet-based high-throughput single-microbe RNA-seq assay (smRandom-seq), using random primers for in situ cDNA generation, droplets barcoding, CRISPR-based rRNA depletion mRNA enrichment. smRandom-seq showed high species specificity (99%), minor doublet...
Microwave thermotherapy (MWT) has shown great potential in cancer treatment due to its deep tissue penetration and minimally invasive nature. However, the poor microwave absorption (MA) properties of thermal sensitizer medical frequency band significantly limit effect MWT then weaken therapeutic efficacy. In this paper, a Ni-based multilayer heterointerface nanomissile MOFs-Ni-Ru@COFs (MNRC) with improved MA performance desired via introducing magnetic loss dielectric is developed for...
The global spread of multidrug-resistant pathogenic fungi presents a serious threat to human health, necessitating the discovery antifungals with unique modes action1. However, conventional activity-based screening for previously undescribed antibiotics has been hampered by high-frequency rediscovery known compounds and lack new antifungal targets2. Here we report polyene antibiotic, mandimycin, using phylogeny-guided natural-product platform. Mandimycin is biosynthesized mand gene cluster,...
Abstract We introduce CUT&RUNTools as a flexible, general pipeline for facilitating the identification of chromatin-associated protein binding and genomic footprinting analysis from antibody-targeted CUT&RUN primary cleavage data. extracts endonuclease cut site information sequences short-read fragments produces single-locus estimates, aggregate motif footprints, informative visualizations to support high-resolution mapping capability CUT&RUN. is available at...
Panax notoginseng, a perennial herb of the genus in family Araliaceae, has played an important role clinical treatment China for thousands years because its extensive pharmacological effects. Here, we report high-quality reference genome P. with size up to 2.66 Gb and contig N50 1.12 Mb, produced third-generation PacBio sequencing technology. This is first chromosome-level assembly Panax. Through evolution analysis, explored phylogenetic whole-genome duplication events examined their impact...
Activation of RIPK1-driven cell death and inflammation play important roles in the progression nonalcoholic steatohepatitis (NASH). However, mechanism underlying RIPK1 activation NASH remains unclear. Here we identified SENP1, a SUMO-specific protease, as key endogenous inhibitor RIPK1. SENP1 is progressively reduced proportion to severity patients. Hepatocyte-specific SENP1-knockout mice develop spontaneous NASH-related phenotypes kinase-dependent manner. We demonstrate that deficiency...
Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes genome. We hypothesized that modulating certain endogenous pathway cells could improve action those editing tools mammalian cells. established a reporter system which small fragment was integrated into genome by (PE). With this system, we screened an in-house small-molecule library identified group histone deacetylase inhibitors...
This paper addresses the intractable problem of attaining precise attitude estimation efficiently using MEMS MARG (magnetic, angular rate, and gravity) sensors for 3-D motion tracking, which is called heading reference system (AHRS). The performance AHRS adversely affected by sensor noise measurement disturbances. To overcome this problem, we propose a novel adaptive extended Kalman filter (AEKF) in paper, including multiplicative (MEKF) hidden Markov Model (HMM) recognizer. MEKF built on...
Macroautophagy/autophagy is elevated to ensure the high demand for nutrients growth of cancer cells. Here we demonstrated that MCOLN1/TRPML1 a pharmaceutical target oncogenic autophagy in cancers such as pancreatic cancer, breast gastric malignant melanoma, and glioma. First, showed activating MCOLN1, by increasing expression channel or using MCOLN1 agonists, ML-SA5 MK6-83, arrests autophagic flux perturbing fusion between autophagosomes lysosomes. Second, regulates mediating release zinc...
Inflammation has profound but poorly understood effects on metabolism, especially in the context of obesity and nonalcoholic fatty liver disease (NAFLD). Here, we report that hepatic interferon regulatory factor 3 (IRF3) is a direct transcriptional regulator glucose homeostasis through induction Ppp2r1b , component serine/threonine phosphatase PP2A, subsequent suppression production. Global ablation IRF3 mice high-fat diet protected against both steatosis dysglycemia, whereas...
Large-scale three-dimensional (3D) CdS nanocrystals with a flowerlike morphology were synthesized by facile hydrothermal treatment from Cd(NO3)2, thiourea, and hexamethylenetetramine [(CH2)6N4, HMT], where HMT acted as capping agent. The reaction conditions influencing the synthesis of these 3D such initial precursor ratios, time, temperatures, agents studied optimized. morphology, structure, phase composition nanostructures examined X-ray diffraction (XRD), field-emission scanning electron...
Alzheimer's disease (AD) is the most common age-related dementia characterized by progressive neuronal loss. However, molecular mechanisms for loss still debated. Here, we used induced pluripotent stem cells (iPSCs) derived from somatic of familial AD patients carrying PSEN1 mutations to study early pathogenic event AD. We found that premature differentiation with decreased proliferation and increased apoptosis occured in AD-iPSC-derived neural progenitor (AD-NPCs) once was initiated,...
FOXO transcription factors have long been associated with longevity control and tissue homeostasis. Although the transcriptional regulation of previously characterized (especially in long-lived insulin mutants under stress conditions), how normal aging impacts activity is poorly understood. Here, we conducted a chromatin immunoprecipitation sequencing (ChIP-Seq) analysis both young (2-week-old) aged (5-week-old) wild-type female fruit flies, Drosophila melanogaster, to evaluate dynamics gene...
The Chinese hamster ovary (CHO) mutant UV40 cell line is hypersensitive to UV and ionizing radiation, simple alkylating agents, DNA cross-linking agents. cells also have a high level of spontaneous chromosomal aberrations 3-fold elevated sister chromatid exchange. We cloned sequenced human cDNA, designated XRCC9 , that partially corrected the hypersensitivity mitomycin C, cisplatin, ethyl methanesulfonate, UV, γ-radiation. in cDNA transformants were almost fully whereas exchanges unchanged....
Degeneration of vessels precedes and precipitates the devastating ischemia many diseases, including retinopathy prematurity diabetic retinopathy. Ischemia then leads to proliferative blindness. Understanding mechanisms blood vessel degeneration is critical prevention these diseases. Vessel loss associated with oxygen-induced suppression vascular endothelial growth factor (VEGF) pericyte (vascular smooth muscle cell) dropout. The molecular mechanism protection vasculature unknown. We show...