A5076522377- Spaceflight effects on biology
- Genetics, Aging, and Longevity in Model Organisms
- High Altitude and Hypoxia
- Connexins and lens biology
- Anesthesia and Neurotoxicity Research
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Radiation Therapy and Dosimetry
- Circadian rhythm and melatonin
- Genomics and Rare Diseases
- Cancer Genomics and Diagnostics
- Retinal Development and Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Nuclear Receptors and Signaling
- Diabetes Treatment and Management
- Medical Imaging Techniques and Applications
- Ion channel regulation and function
- Glaucoma and retinal disorders
- Cardiovascular Health and Disease Prevention
- Retinal Imaging and Analysis
- Sleep and Wakefulness Research
- Retinal Diseases and Treatments
- Cardiac Ischemia and Reperfusion
- Corneal surgery and disorders
- Effects of Radiation Exposure
Loma Linda University
2019-2024
Loma Linda University Medical Center
2019-2021
University of California, Los Angeles
2014
Western University
2006-2014
Abstract Extended spaceflight has been shown to adversely affect astronaut visual acuity. The purpose of this study was determine whether alters gene expression profiles and induces oxidative damage in the retina. Ten week old adult C57BL/6 male mice were flown aboard ISS for 35 days returned Earth alive. Ground control maintained on under identical environmental conditions. Within 38 (+/−4) hours after splashdown, ocular tissues collected analysis. RNA sequencing detected 600 differentially...
Many factors contribute to the health risks encountered by astronauts on missions outside Earth's atmosphere. Spaceflight-induced potential adverse neurovascular damage and late neurodegeneration are a chief concern. The goal of present study was characterize effects spaceflight oxidative in mouse brain its impact blood-brain barrier (BBB) integrity. Ten-week-old male C57BL/6 mice were launched International Space Station (ISS) for 35 days as part Space-X 12 mission. Ground control (GC)...
Abstract The health risks associated with spaceflight-induced ocular structural and functional damage has become a recent concern for NASA. goal of the present study was to characterize effects spaceflight reentry 1 g on structure integrity retina blood-retinal barrier (BRB) in eye. To investigate possible mechanisms, changes protein expression profiles were examined mouse tissue after spaceflight. Ten week old male C57BL/6 mice launched International Space Station (ISS) Space-X 12 at...
There is concern that degradation of vision as a result space flight may compromise both mission goals and long-term quality life after travel. The visual disturbances be due to combination intracerebral pressure changes exposure ionizing radiation. retina the retinal vasculature play important roles in vision, yet have not been studied extensively relationship travel goal current study was characterize oxidative damage apoptosis endothelial cells whole-body gamma-ray, proton oxygen (16O)...
The purpose of the current study was to characterize effects simulated microgravity and radiation-induced changes in retina retinal vasculature, assess accompanying early immune cells hematological parameters. To better understand spaceflight, we used a combination treatments designed simulate both radiation low-gravity aspects space conditions. broad energy spectrum large solar particle event (SPE) galactic cosmic ray (GCR) radiation, male C57BL/6J mice were exposed whole-body irradiation...
Spaceflight poses many challenges for humans. Ground-based analogs typically focus on single parameters of spaceflight and their associated acute effects. This study assesses the long-term transcriptional effects following combination analog conditions using mouse model: simulated microgravity via hindlimb unloading (HLU) and/or low-dose γ-ray irradiation (LDR) 21 days, followed by 4 months readaptation. Changes in gene expression epigenetic modifications brain samples during readaptation...
The goal of the present study was to characterize acute oxidative damage in ocular structure and retinal function after exposure spaceflight, evaluate efficacy an antioxidant reducing spaceflight-induced changes retina. Ten-week-old adult C57BL/6 male mice were flown aboard ISS on Space-X 24 over 35 days, returned Earth alive. received a weekly injection superoxide dismutase mimic, MnTnBuOE-2-PyP 5+ (BuOE), before launch during their stay onboard ISS. Ground control maintained under...
It has been proposed that neuroinflammatory response plays an important role in the neurovascular remodeling brain after stress. The goal of present study was to characterize changes gene expression profiles associated with neuroinflammation, neuronal function, metabolism and stress mouse tissue. Ten-week old male C57BL/6 mice were launched International Space Station (ISS) on SpaceX-12 for a 35-day mission. Within 38 ± 4 h splashdown, returned Earth alive. Brain tissues collected analysis....
Abstract Astronauts exhibit an assortment of clinical abnormalities in their eyes during long-duration spaceflight. The purpose this study was to determine whether spaceflight induces epigenomic and transcriptomic reprogramming the retina or alters epigenetic clock. mice were flown for 37 days animal enclosure modules on International Space Station; ground-based control animals maintained under similar housing conditions. Mouse retinas isolated both DNA methylome transcriptome determined by...
The development of eye pathology is a serious concern for astronauts who spend time in deep space. Microgravity major component the spaceflight environment which could have adverse effects on ocular health. use centrifugation to exert forces that partially or fully mimic Earth-level gravity space possible countermeasure mitigate microgravity eye. Therefore, we subjected mice International Space Station (ISS) (0 G) artificial by at 0.33 G, 0.67 and 1 then performed RNA sequencing (RNA-seq)...
Connexin 43, the major connexin isoform in gap junctions of cardiac ventricular myocytes, undergoes changes distribution and expression diseases. The Na<sup>+</sup>-H<sup>+</sup> exchanger (NHE-1), a key mediator hypertrophy heart failure, has been shown to be localized cardiomyocyte junctional regions; however, whether NHE-1 regulates junction proteins hypertrophied is not known. To address this question, neonatal rat myocytes were treated with phenylephrine (PE) for 24 h induce...
The demands of deep space pose a health risk to the central nervous system that has long been concern when sending humans space. While little is known about how spaceflight affects transcription spatially in brain, greater understanding this process potential aid strategies mitigate effects on brain. Therefore, we performed GeoMx Digital Spatial Profiling mouse brains subjected either or grounded controls. Four brain regions were selected: Cortex, Frontal Corunu Ammonis I, and Dentate Gyrus....
Abstract We characterized two reference samples for NGS technologies: a human triple-negative breast cancer cell line and matched normal line. Leveraging several whole-genome sequencing (WGS) platforms, multiple replicates, orthogonal mutation detection bioinformatics pipelines, we minimized the potential biases from technologies, assays, informatics. Thus, our “truth sets” were defined using evidence 21 repeats of WGS runs with coverages ranging 50X to 100X (a total 140 billion reads)....
There are serious concerns about possible late radiation damage to ocular tissue from prolonged space exposure, and occupational medical procedures. This study aimed investigate the effects of whole-body high-energy proton exposure at a single dose on apoptosis, oxidative stress, blood-retina barrier (BRB) integrity in retina optic nerve head (ONH) region compare these radiation-induced with those produced by fractionated dose. Six-month-old C57BL/6 male mice were either sham irradiated or...
Reports show that prolonged exposure to a spaceflight environment produces morphologic and functional ophthalmic changes in astronauts during after an International Space Station (ISS) mission. However, the underlying mechanisms of these spaceflight-induced are currently unknown. The purpose present study was determine impact on ocular structures by evaluating thickness mouse retina, retinal pigment epithelium (RPE), choroid sclera layer using micro-CT imaging. Ten-week-old C57BL/6 male mice...
The health risk of flight condition-triggered ocular injury and neurodegeneration has long been a concern. Spaceflight missions will likely expose the astronauts experiment payloads to greater radiation levels compared those encountered on Earth. Knowledge about susceptibility adverse effects from low doses during space is very limited. Our present study aims investigate compare whole-body simulated galactic cosmic rays (GCR) blood-retinal barrier (BRB) integrity, oxidative stress, apoptosis...
Reports show that prolonged exposure to a spaceflight environment produces morphologic and functional ophthalmic changes in astronauts during after an International Space Station (ISS) mission. However, the underlying mechanisms of these spaceflight-induced are currently unknown. The purpose present study was determine impact on ocular structures by evaluating thickness mouse retina, retinal pigment epithelium (RPE), choroid sclera layer using micro-CT imaging. Ten-week-old C57BL/6 male mice...
Connexin 43, the major gap junction protein expressed in cardiac ventricular cells, undergoes changes distribution and expression levels diseases. The Na-H exchanger (NHE1), a key mediator of hypertrophy heart failure, is colocalized with Cx43 cells. Neonatal rat myocytes were treated phenylephrine (PE) for 24 hours to induce hypertrophy. Increased observed PE treatment (35 ± 6% compared control) was further significantly augmented by NHE1 inhibitor EMD87580 (75 9 % control). As assessed...
Abstract Astronauts exhibit an assortment of clinical abnormalities in their eyes during long-duration spaceflight. The purpose this study was to determine whether spaceflight induces epigenomic and transcriptomic reprogramming the retina or alters epigenetic clock. mice were flown for 37 days animal enclosure modules on International Space Station; ground-based control animals maintained under similar housing conditions. Mouse retinas isolated both DNA methylome transcriptome determined by...