A5028595728- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Cell Adhesion Molecules Research
- Cardiac Structural Anomalies and Repair
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Fatty Acid Research and Health
- Coronary Artery Anomalies
- Animal Virus Infections Studies
- Biochemical effects in animals
- Cardiovascular Disease and Adiposity
- Angiogenesis and VEGF in Cancer
- Advanced Surface Polishing Techniques
- Genetics and Neurodevelopmental Disorders
- Cellular Mechanics and Interactions
- thermodynamics and calorimetric analyses
- Cardiovascular Effects of Exercise
- RNA modifications and cancer
- Renal and related cancers
- Neuroinflammation and Neurodegeneration Mechanisms
Guangzhou Medical University
2019-2022
South China University of Technology
2022
Guangzhou Women and Children Medical Center
2021
University of California, Los Angeles
2014-2016
Broad Center
2016
Indiana University School of Medicine
2012
University of North Carolina at Chapel Hill
2007-2011
UNC Lineberger Comprehensive Cancer Center
2011
Chinese Academy of Sciences
2004-2006
Institute of Biophysics
2004-2006
Abstract Aims Circular RNAs (circRNAs) are involved in gene regulation a variety of physiological and pathological processes. The present study aimed to investigate the effect circRNA_000203 on cardiac hypertrophy potential mechanisms involved. Methods results CircRNA_000203 was found be up-regulated myocardium Ang-II-infused mice cytoplasma Ang-II-treated neonatal mouse ventricular cardiomyocytes (NMVCs). Enforced expression enhances cell size atrial natriuretic peptide β-myosin heavy chain...
Ischemic heart disease is a major global public health challenge, and its functional outcomes remain poor. Lysine crotonylation (Kcr) was recently identified as post-translational histone modification that robustly indicates active promoters. However, the role of Kcr in myocardial injury unknown. In this study, we aimed to clarify pathophysiological significance cardiac explore underlying mechanism. We investigated dynamic change both sites protein level left ventricular tissues at 2 time...
A novel coronavirus, SARS-CoV, has been confirmed to be the aetiological agent of SARS. Transmission electron microscope (TEM) images played an important role in initial identification pathogen. In order obtain greater morphological detail SARS-CoV than could obtained by TEM, we used ultra-high resolution scanning microscopy (SEM) image virus particles. We show here three-dimensional appearance SARS-CoV. Enhanced ultrastructure reveals trimeric structure 10-20 nm spikes on virion surface....
Nucleobindin‐1 (NUCB1) and nucleobindin‐2 (NUCB2) are multifunctional proteins that interact with Ca 2+ , nucleic acids various regulatory in different signaling pathways. So far, our understanding of the regulation biological functions nucleobindins remains limited. In proteome‐wide selection for downstream caspase substrates, both NUCB1 NUCB2 found to be substrates caspases. We report here detailed analyses cleavage by Significantly, sites located exactly at one ‐binding EF‐hand motifs....
Human endothelial progenitor cells (hEPCs) participate in neovascularization of ischemic tissues. Function and number hEPCs decline patients with cardiovascular disease, therapeutic strategies to enhance hEPC function remain an important field investigation. The Wnt signaling system, comprising 19 lipophilic proteins, regulates vascular patterning the developing embryo. However, effects Wnts on adult vasculature unclear. We demonstrate here that Wnt1 is expressed a subset lining murine...
MicroRNA-199a-5p (miR-199a-5p) and -3p are enriched in the myocardium, but it is unknown whether miR-199a-5p co-expressed cardiac remodeling what roles they have hypertrophy fibrosis. We show that co-upregulated mouse human myocardium with Ang-II-treated neonatal ventricular cardiomyocytes (NMVCs) fibroblasts (CFs). could aggravate fibrosis vivo vitro. PPAR gamma coactivator 1 alpha (Ppargc1a) sirtuin (Sirt1) were identified as target genes to mediate promoting both However, miR-199a-3p...
The role of Mesenchymal-endothelial transition (MEndoT) in cardiac hypertrophy is unclear. To determine the difference between MEndoT-derived and coronary endothelial cells essential for understanding revascularizing strategy repair. Using lineage tracing we demonstrated that exhibit highly heterogeneous which were characterized with expression markers such as vascular cadherin(VECAD) occludin but low Tek receptor tyrosine kinase(Tek), isolectin B4, nitric oxide synthase(eNOS), von...
Significant efforts have been made to improve the target-binding strength of numerous affinity molecules that are widely used in biomedical research and clinical applications. While many antibody-like fragments successfully optimized through maturation, process is time-consuming restricted by stability, solubility, expression level protein sequences. To generate a fibronectin type III domain (FN3) based monobody binds tumor-related biomarkers with exceptional high we developed multivalent...
Rational: Wnt4 plays a critical role in development and is reactivated during fibrotic injury; however, the of cardiac repair remains unclear.In this study, our aim was to clarify pathophysiological mechanisms following acute ischemic reperfusion injury. Methods results:We investigated spatio-temporal expression injury found that upregulated as an early response gene fibroblasts near border zone associated with mesenchymal-endothelial transition (MEndoT), beneficial process for...
Diacylglycerol (DAG)-based edible oils have attracted increasing research interest owing to their health-promoting properties. Recent animal and human studies showed that an increased 1,2-DAG content in the liver skeletal muscle may cause insulin resistance. However, earlier using models or humans reported dietary DAGs with a 1,2-DAGs 1,3-DAGs ratio of approximately 3:7 could improve sensitivity type 2 diabetic patients. This conflict raises question whether there is link between ingested...
An antibody phage-display library was constructed from the B cells of convalescent severe acute respiratory syndrome (SARS) patients and screened using inactivated SARS coronavirus (CoV) virions as antigens. More than 80 positive clones were isolated one them, scFv H12, extensively characterized. H12 bound to SARS-CoV with high affinity (equilibrium dissociation constant, Kd=73.5 nM), neutralized in vitro. The facts that SARS-S1 protein under non-reducing conditions it did not bind monomeric...
Childhood obesity, as one of the potential risk factors cardiovascular diseases, is closely associated with incidence disease at a younger age and has become public health concern worldwide. However, its effects on system have still remained elusive. In this study, we systematically evaluated characteristics 79 obese children 161 normal weight in Guangzhou (China) using biomarkers for disease. Compared children, not only exhibited significantly higher levels creatine kinase (CK), lactate...
The electrical properties of electronic materials are greatly affected by their microstructure, which in turn is determined the processes used for fabrication. Transmission Electron Microscopy (TEM) one most powerful analytical techniques to provide localized information about microstructure as it relates process control parameters. This development and failure diagnoses well QA/QC control. In this study, microstructures within devicesof polysilicon microresistors were analyzed using TEM...