A5039101703- Sugarcane Cultivation and Processing
- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- Molecular Biology Techniques and Applications
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant-derived Lignans Synthesis and Bioactivity
- Salivary Gland Disorders and Functions
- Plant Stress Responses and Tolerance
- Proteins in Food Systems
- Cocoa and Sweet Potato Agronomy
- RNA Research and Splicing
- Plant Virus Research Studies
- Glycosylation and Glycoproteins Research
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Plant pathogens and resistance mechanisms
- Body Composition Measurement Techniques
Agronomical Institute of Campinas
2015-2024
Universidade Federal de São Paulo
2018-2024
Universidade de São Paulo
2001-2023
Universidade Estadual de Campinas (UNICAMP)
2012-2017
SUPERA Park of Innovation and Technology of Ribeirão Preto
2015-2016
The Ohio State University
2014-2015
Universidade de Ribeirão Preto
2011-2015
Universidade Estadual Paulista (Unesp)
2015
University of Chile
2007-2008
Austral University of Chile
1977-2007
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. has an unusually broad host range, extensive geographical distribution throughout the American continent, induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of isolates were expected to be genetically divergent. Here we report genome sequence (Temecula...
Treatment of caput or cauda epididymal rat sperm with a low concentration (0.05%) the cationic detergent cetyltrimethylammonium bromide and 30 mM 2-mercaptoethanol solubilized most structures except for head outer dense fiber-connecting piece complex. The latter were purified, about 10% these complexes are formed by nine fibers attached to connecting piece. Of fibers, two shorter than other seven presumably correspond 3 8 (Fawcett, D.W. (1975) Dev. Biol. 44, 394-436). Electron microscopy...
Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce second-generation ethanol. Lignin major factor limiting use bagasse other plant materials biosynthesis pathway complex network changes in expression genes this have general led diverse undesirable impacts on structure physiology. Despite its economic importance, genome was still...
Establishing the architecture of gene regulatory networks (GRNs) responsible for controlling transcription all genes in an organism is a natural development that follows elucidation genome sequence. Reconstruction GRN requires availability series molecular tools and resources so far have been limited to few model organisms. One such resource consists collections factor (TF) open reading frames (ORFs) cloned into vectors facilitate easy expression plants or microorganisms. In this study, we...
Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among abiotic stress, drought one that negatively impact sugarcane yield. Although gene expression analysis through quantitative PCR (qPCR) has increased our knowledge about biological processes related to drought, network mediates responses water deficit remains elusive. In such scenario, validation of reference a major requirement successful analyzes involving qPCR.In this study, candidate genes were...
The lignin deposition in the stem of two sugarcane genotypes was assessed on exposure to water stress. content and morphoanatomical characterization indicated that IACSP94-2094 plants are more lignified than those IACSP95-5000 genotype, under normal supply conditions, which especially associated with higher contents rind mature internodes. Water deficit had negative impact biomass production, mostly plants, possibly due stress severity or susceptibility genotype during stem-lengthening...
<h3>Objectives:</h3> MUC5B contains sulfated and sialylated oligosaccharides that sequester water required for moisturising the oral mucosa. Xerostomia, in patients with Sjögren syndrome, is generally associated reduced quantities, rather than altered properties, of saliva. Here, we determined amount (mRNA protein) as well sulfation levels salivary glands normal or unstimulated flow. Localisation MUC5B, total groups were compared acini basal lamina disorganisation. <h3>Patients methods:</h3>...
The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These vary depending stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model stigma, and its stigmas/styles express genes to accomplish appropriate functions. For large-scale study gene expression during tobacco pistil development preparation for pollination, we generated 11,216 high-quality expressed sequence tags (ESTs) from created TOBEST database. ESTs were assembled in...
Abstract Background The genus Eucalyptus consists of approximately 600 species and subspecies has a physiological plasticity that allows some to propagate in different regions the world. is major source cellulose for paper manufacturing, its cultivation limited by weather conditions, particularly water stress low temperatures. Gene expression studies using quantitative reverse transcription polymerase chain reaction (qPCR) require reference genes, which must have stable facilitate comparison...
Drought is the most significant environmental stress for agricultural production worldwide, and tremendous efforts have been made to improve crop yield under increasing water scarcity. Transcription factors are major players in regulation of stress-related genes plants. Recently, different MYB transcription were characterized their involvement drought response. A sugarcane R2R3-MYB gene (ScMYBAS1) its four alternative forms transcript (ScMYAS1-2, ScMYBAS1-3, ScMYBAS1-4 ScMYBAS1-5) identified...
Abstract We used primers designed on conserved gene regions of several species to isolate the most expressed genes lignin pathway in four Saccharum species. S. officinarum and barberi have more sucrose culms than spontaneum robustum , but less polysaccharides cell wall. had lowest S/G ratio a lower rate saccharification mature internodes. Surprisingly, except for CAD, 4CL, CCoAOMT which we found three, two, two genes, respectively, only one was other enzymes their sequences were highly...
Sugarcane (Saccharum spp.) is an important economic crop for both sugar and biomass, the yields of which are negatively affected by flowering. The molecular mechanisms controlling flowering in sugarcane nevertheless poorly understood. RNA-seq data analysis database searches have enabled a comprehensive description PEBP gene family sugarcane. It shown to consist at least 13 FLOWERING LOCUS T (FT)-like genes, two MOTHER OF FT AND TFL (MFT)-like four TERMINAL FLOWER (TFL)-like genes. As...
See also the Commentary by Nieuwland et al
Abstract Although reference genes have previously been used in the expression analysis of involved sugarcane flowering they had not experimentally validated for stability and consistency between different samples over a wide range experimental conditions. Here we report candidate tissue types, at temporal time-points, both short long day photoperiodic treatments. The all conditions was evaluated with NormFinder, BestKeeper, RefFinder algorithms that complement each other more robust...
Successful plant reproduction and fruit formation depend on adequate pollen pistil development, pollen-pistil interactions. In Nicotiana tabacum, tubes grow through the intercellular spaces of pistil-specialized tissues, stigmatic secretory zone, stylar transmitting tissue (STT). These are supposed to be formed by modulation cell wall pectin esterification. Previously we have identified a gene preferentially expressed in pistils encoding putative acetylesterase (PAE), named NtPAE1. Here,...
A sugarcane gene encoding a dirigent-jacalin, ShDJ, was induced under drought stress. To elucidate its biological function, we integrated ShDJ-overexpression construction into the rice Nipponbare genome via Agrobacterium-mediated transformation. Two transgenic lines with single copy in T0 were selected and evaluated both T1 T4 generations. Transgenic had drastically improved survival rate water deficit conditions, at rates close to 100%, while WT did not survive. Besides, biomass production...
<h3>Objectives:</h3> In salivary glands from patients with Sjögren syndrome, overexpression of laminins 1 and 5 disorganisation the acinar basal lamina have been reported. Laminin mediates association epithelial cells by forming adhesion complexes upon interaction α6β4 integrin. present work, mRNA protein levels integrin were determined its localisation in evaluated syndrome. <h3>Methods:</h3> Salivary 12 syndrome 8 controls studied. The semiquantitative reverse transcriptase (RT)-PCR...
In Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum to determine the developmental timing of important events preparation for successful fertilization. First, we performed hand pollinations flowers from stages 4 11 study which stage pollination would fruits. A Peroxtesmo test was correlate peroxidase activity on stigma...
Aquaporins (AQPs) are proteins that facilitate the transport of water, small neutral solutes and gases across membranes have an important role in plant physiology drought stress responses. The sugarcane (Saccharum spp.) transcriptome was searched for AQPs, also known as major intrinsic proteins. Phylogenetic analysis based on nucleotide sequences identified 33 isoforms fit into four AQP subfamilies previously described monocotyledonous: 13 plasma membrane protein (PIPs), 11 tonoplast (TIPs),...