A5054305299- Salivary Gland Disorders and Functions
- Social Sciences and Policies
- Glycosylation and Glycoproteins Research
- Education and Teacher Training
- Galectins and Cancer Biology
- Aging, Health, and Disability
- Social Issues and Sustainability
- Endoplasmic Reticulum Stress and Disease
- Salivary Gland Tumors Diagnosis and Treatment
- Public Policy and Governance
- Public Health and Social Inequalities
- Income, Poverty, and Inequality
- International Relations in Latin America
- Economic and Social Development
- Latin American socio-political dynamics
- Plant Molecular Biology Research
- Ethics and bioethics in healthcare
- Plant Genetic and Mutation Studies
- Poverty, Education, and Child Welfare
- Chromosomal and Genetic Variations
- Political and Social Dynamics in Chile and Latin America
- Health and Medical Education
- Immune Cell Function and Interaction
- Selenium in Biological Systems
- Migration, Education, Indigenous Social Dynamics
University of Chile
2013-2025
Universidad de Los Lagos
2018-2021
Universidad Católica Silva Henríquez
2020-2021
Pontificia Universidad Católica de Chile
2007-2020
University of Bristol
2015-2017
Alberto Hurtado University
2016
Queen's University
2011
Centro de Investigaciones Biológicas Margarita Salas
1997
<h3>Objectives:</h3> MUC5B contains sulfated and sialylated oligosaccharides that sequester water required for moisturising the oral mucosa. Xerostomia, in patients with Sjögren syndrome, is generally associated reduced quantities, rather than altered properties, of saliva. Here, we determined amount (mRNA protein) as well sulfation levels salivary glands normal or unstimulated flow. Localisation MUC5B, total groups were compared acini basal lamina disorganisation. <h3>Patients methods:</h3>...
To evaluate the expression and localization of MUC1/SEC MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well their vitro induced by cytokines.Labial gland 27 primary SS 22 non-SS sicca subjects were studied. Relative mRNA levels determined qPCR protein Western blotting. Induction mucin mRNAs was assayed vitro. Immunohistochemistry used for localization.Relative significantly higher LSG patients. These cytokines. detected acini apical region...
Objectives. To determine the expression and enzymatic activities of sulphotransferases involved in mucin hyposulphation labial salivary glands (LSGs) from SS patients to correlate sulphotransferase activity with clinical parameters such as secretion, inflammation serology. Methods. LSG 31 control subjects were studied. Relative mRNA protein levels Gal3-O-sulphotransferases (Gal3STs) β1,3-galactosyltransferase-5 (β3GalT5) determined by quantitative RT-PCR western blotting, respectively....
Abstract 10 −6 M and −5 5‐azacytidine, demethylated around 9% 17% of the 5‐methylcytosine residues found in Allium cepa L. native DNA, respectively. Both treatments stimulated RNA synthesis cells root meristems. On other hand, treatment gave rise to multiple chromosomal anomalies mitosis before any fall mitotic index was detectable, but no breaks were ever seen. Serious lesions involved chromatids segregation anaphase preferentially after hypomethylation DNA sequences replicated second half...
<h3>Objectives:</h3> In salivary glands from patients with Sjögren syndrome, overexpression of laminins 1 and 5 disorganisation the acinar basal lamina have been reported. Laminin mediates association epithelial cells by forming adhesion complexes upon interaction α6β4 integrin. present work, mRNA protein levels integrin were determined its localisation in evaluated syndrome. <h3>Methods:</h3> Salivary 12 syndrome 8 controls studied. The semiquantitative reverse transcriptase (RT)-PCR...
Objectives: To compare differences in depression, loneliness and personal well-being a sample made up of indigenous (Aymara Mapuche) non-indigenous older people resident original rural territories. Methods: A cross-sectional study involving 800 adults living context Chile, whom 201 were Aymara, 368 Mapuche 231 non-indigenous. Validated instruments included for (outcome variables). Ordinary least squares regression analyses performed. Results: Membership an group was significantly associated...
The aim of this article is to make an invitation revisit the contributions Latour (along others such as Callon, Star, and Marres) in order widen inputs that a policy translation approach can provide, particularly what pertains analysis processes movement ideas between multiple actors process implementation social policies. To do this, organized five sections. first section aims situate scenario which associated with connected actor-network theory have influenced discussions analysis,...
Es notable la escasez de análisis en torno a realización derechos los servicios sociales, más allá dimensión su calidad. El trabajo busca hacer una contribución al desarrollo sistemas integrales protección social con enfoque derechos, colocando el foco aplicación y naturaleza multidimensional derechos. A tales efectos, construye un marco conceptual discute resultados investigación empírica que incluye documental cuestionarios aplicados directivos usuarios dos cuidado dirigidos personas...
Los arreglos institucionales a través de los cuales se implementan servicios sociales pueden afectar la medida en que derechos reconocidos son realmente ejercidos por las personas. Dado lo inexplorado esta temática realizó un catastro dirigidos infancia y adultos mayores tres comunas región metropolitana Chile. Se evidencia mayoría estos grupos ejecutados bajo asociaciones público privadas (APP), hay una presencia significativa organizaciones no mercantiles así como gran heterogeneidad tipos...
<h3>Background</h3> Labial-salivary-glands (LSG) of Sjögren9s syndrome (SS) patients show an altered exocytic route that can induce endoplasmic reticulum (ER)-stress and activate the unfolded-protein-response (UPR)<sup>1,2</sup>. UPR helps to recover preserve cellular homeostasis, reducing load unfolded proteins in ER, through various mechanisms cell survival. is mediated by activation IRE1α, PERK ATF6 sensors.Chronic ER-stress sensors exhibits a time course-kinetics with attenuation IRE1α...
<h3>Background</h3> Labial salivary glands (LSG) of Sjögren9s syndrome (SS) patients show alterations indicative endoplasmic reticulum (ER) stress, such as ER cistern dilatation (1) and mucin accumulation (2). Pro-inflammatory cytokines induce but it is not well known if the stress an initial event in onset a chronic disease or secondary to inflammation. triggers complementary adaptive mechanism "Unfolded Protein Response" (UPR) that seeks restore homeostasis. IRE1α/XBP-1 signaling pathway...
La noción de pruebas y soportes, inscritas en la sociología del individuo, permite vincular situaciones complejas carácter histórico estructural que afectan vida los individuos (pruebas), con las respuestas despliegan para enfrentarlas (soportes). Mediante un estudio cualitativo exploratorio chilenos migrantes residentes Santiago, Chile, buscamos identificar, desde percepción afectados, principales problemas producidos y/o reforzados por pandemia COVID-19 entre años 2020 2021, modos...
El enfoque de derechos plantea el desafío por establecer indicadores para medir su nivel cumplimiento en diversos contextos y públicos. Hasta ahora, principal énfasis ha sido la calidad equidad las políticas, no un abordaje integral los provisión servicios. Este estudio analiza garantía educación inicial desde una perspectiva multidimensional, identificando diferencias existentes entre arreglos institucionales que proveen este servicio Chile. Desde cuantitativo carácter...
ResumenEste artículo analiza estrategias de promoción compromiso escolar factibles implementar en Chile, el marco un sistema diagnóstico, monitoreo y evaluación del que ya cuenta con una plataforma tecnológica para su evaluación.Este sistema, validación ajuste instrumentos factores contextuales facilitadores algunos países iberoamericanos e incorporará coherentes fortalecer escolar.Para ello, este trabajo sistematizó 43 basadas evidencia contexto internacional realizó proceso mediante juicio...
<h3>Background</h3> Labial salivary glands (LSG) from Sjögren9s syndrome (SS) patients show impaired cell-polarity, alterations of SNARE proteins localization and presence mucins in the extracellular matrix. Exocytosis Ca<sup>+</sup> signaling are polarized processes acinar-cells. Synaptotagmin-I (Syt-I) is a Ca<sup>+</sup>-sensor protein localized membrane secretory granules linker between stimulus exocytic machinery. A significant upregulation SytI observed microarray assay response less...