A5015720759- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- HIV Research and Treatment
- Sugarcane Cultivation and Processing
- Enzyme-mediated dye degradation
- Brazilian Legal Issues
- Plant responses to water stress
- HIV/AIDS drug development and treatment
- Biochemical and biochemical processes
- Plant Molecular Biology Research
- Public Health in Brazil
- HIV/AIDS Research and Interventions
- Plant Surface Properties and Treatments
- Bacteriophages and microbial interactions
- Postharvest Quality and Shelf Life Management
- Environmental Sustainability and Education
- Plant Micronutrient Interactions and Effects
- Genomics and Phylogenetic Studies
- Rice Cultivation and Yield Improvement
- Polysaccharides and Plant Cell Walls
- Wastewater Treatment and Reuse
- Iron Metabolism and Disorders
- Plant tissue culture and regeneration
- Mycobacterium research and diagnosis
Federal Center for Technological Education of Minas Gerais
2025
Universidade Estadual de Campinas (UNICAMP)
2012-2024
University of Minho
2019-2021
Ghent University
2013-2016
VIB-UGent Center for Plant Systems Biology
2016
Universidade Federal do Rio de Janeiro
2014-2015
Universidade de Brasília
2014
Vlaams Instituut voor Biotechnologie
2013
Internet Society
2009
Lignin Biosynthesis Complications is a polymer that lends its sturdy properties to wood and makes plant cell walls tougher, which creates problems for chemists converting cellulosic biomass into biofuels. Vanholme et al. (p. 1103 , published online 15 August; see the cover) have identified new step in biosynthetic pathway of lignin Arabidopsis caffeoyl shikimate esterase catalyzes synthesis caffeate. Cellulose from mutant plants, had reduced amounts lignin, was more efficiently processed glucose.
Lignin, after cellulose, is the second most abundant biopolymer on Earth, accounting for 30% of organic carbon in biosphere. It considered an important evolutionary adaptation plants during their transition from aquatic environment to land, since it bestowed early tracheophytes with physical support stand upright and enabled long-distance transport water solutes by waterproofing vascular tissue. Although essential plant growth development, lignin major cell wall component responsible biomass...
Lignin is a complex phenolic heteropolymer deposited in the secondarily thickened walls of specialized plant cells to provide strength for plants stand upright and hydrophobicity conducting long-distance water transport. Although essential growth development, lignin major cell-wall component responsible biomass recalcitrance industrial processing. Peroxidases laccases are generally thought be polymerization, but, given their broad substrate specificities large gene families, specific...
Auxin steers numerous physiological processes in plants, making the tight control of its endogenous levels and spatiotemporal distribution a necessity. This regulation is achieved by different mechanisms, including auxin biosynthesis, metabolic conversions, degradation, transport. Here, we introduce cis-cinnamic acid (c-CA) as novel unique addition to small group molecules affecting planta concentrations. c-CA photo-isomerization product phenylpropanoid pathway intermediate trans-CA (t-CA)....
In the xylem of angiosperm plants, microscopic pits through secondary cell walls connect water-conducting vessels. Cellulosic meshes originated from primary walls, and middle lamella between adjacent vessels, called pit membrane, separates one conduit another. The intricate structure nano-sized pores in membranes enables passage water under negative pressure without hydraulic failure due to obstruction by gas bubbles (i.e. embolism) normal conditions or mild drought stress. Since chemical...
Plant biomass has been suggested as an alternative to produce bioethanol. The recalcitrance of plant convert cellulose into simpler carbohydrates used in the fermentation process is partially due lignin, but standard methods analyze lignin composition frequently use toxic solvents and are laborious time-consuming. MS imaging was study Eucalyptus, since this genus main source world. Hand-cut sections stems two Eucalyptus species were covered with silica directly analyzed by matrix-assisted...
Abstract Background and Aims Improving drought adaptation is more pressing for crops such as sugarcane, rice, wheat maize, given the high dependence of these on irrigation. One option enhancing to water limitation in plants by transgenic approaches. An increasing number genes that are associated with mechanisms used cope scarcity have been discovered. Genes encoding proteins unknown functions comprise a relevant fraction modulated drought. We characterized gene response environmental...
Abstract Background The genus Eucalyptus consists of approximately 600 species and subspecies has a physiological plasticity that allows some to propagate in different regions the world. is major source cellulose for paper manufacturing, its cultivation limited by weather conditions, particularly water stress low temperatures. Gene expression studies using quantitative reverse transcription polymerase chain reaction (qPCR) require reference genes, which must have stable facilitate comparison...
Abstract We used primers designed on conserved gene regions of several species to isolate the most expressed genes lignin pathway in four Saccharum species. S. officinarum and barberi have more sucrose culms than spontaneum robustum , but less polysaccharides cell wall. had lowest S/G ratio a lower rate saccharification mature internodes. Surprisingly, except for CAD, 4CL, CCoAOMT which we found three, two, two genes, respectively, only one was other enzymes their sequences were highly...
Drought is considered the major abiotic stress limiting crop productivity. This study seeks to identify proteins involved in drought response sugarcane stems submitted stress. The integration of nuclei enrichment sample preparation with shotgun proteomic approach results great coverage stem proteome 5381 protein groups identified. A total 1204 differentially accumulated are detected drought, among which 586 and 618 increased reduced abundance, respectively. 115 exclusive detected, being 41...
The present study aims to apply the principles of circular economy by utilizing special inorganic waste, specifically gypsum sludge from a Zn refinery produce ceramic tiles. Samples containing up 50% were prepared as partial replacements traditional materials such clay, feldspar, quartz, and kaolin. properties mixtures analysed in accordance with ABNT NBR ISO 10545 standards, including tests for water absorption, flexural strength, stain chemical resistances deep abrasion resistance (ASTM...
Abstract Sugarcane is a highly efficient biomass producer used in the last decades for bioethanol and bioelectricity production, as well animal feeding. Together with lignin, suberin major factor low sugarcane digestibility by ruminants. The lipid–phenolic biomolecular composition ultrastructure of associated waxes confer them extraordinary properties hydrophobicity, flexibility, anti‐microbial resistance, responsible suberized tissues. Additionally, hemicelluloses cross‐linked lignin also...
Summary There is an increasing need for renewable energy sources to replace part of our fossil fuel‐based economy and reduce greenhouse gas emission. Sugarcane bagasse a prominent feedstock produce cellulosic bioethanol, but strategies are still needed improve the cost‐effective exploitation this potential source. In model plants, it has been shown that GUX genes involved in cell wall hemicellulose decoration, adding glucuronic acid substitutions on xylan backbone. Mutation increases enzyme...
HIV-1 subtypes associate with differences in transmission and disease progression. Thus, the existence of geographic hotspots subtype diversity deepens complexity HIV-1/AIDS control. The already high Portugal seems to be increasing due infections sub-subtype A1 virus. We performed phylogenetic analysis 65 sequences newly obtained from 14 Portuguese hospitals 425 closely related database sequences. 80% isolates gathered a main clade (MA1). Six clusters were identified MA1, encompassing...
The extensive genetic diversity of HIV-1 is a major challenge for the prevention and treatment infections. Subtype C accounts most infections in world but has been mainly localized Southern Africa, Ethiopia India. For elusive reasons, South Brazil harbors largest subtype epidemic American continent that elsewhere dominated by B. To investigate this topic, we collected clinical data viral sequences from 2611 treatment-naïve patients diagnosed with Brazil. Molecular epidemiology analysis...
The success of antiretroviral treatment (ART) is threatened by the emergence drug resistance mutations (DRM). Since Brazil presents largest number people living with HIV (PLWH) in South America we aimed at understanding dynamics DRM this country. We analyzed a total 20,226 HIV-1 sequences collected from PLWH undergoing ART between 2008–2017. Results show mild decline over years but an increase K65R reverse transcriptase mutation 2.23% to 12.11%. This gradually occurred following alterations...
Recalcitrance of plant biomass is closely related to the presence phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus main source wood for pulp paper industry. However, when compared model plants such as Arabidopsis thaliana poplar, relatively little known about biosynthesis only few genes were functionally characterized. An efficient, fast inexpensive vitro...
Abstract Human immunodeficiency virus 1 (HIV-1) genome sequencing is routinely done for drug resistance monitoring in hospitals worldwide. Subtyping these extensive datasets of HIV-1 sequences a critical first step molecular epidemiology and evolution studies. The clinical relevance subtypes increasingly recognized. Several studies suggest subtype-related differences disease progression, transmission route efficiency, immune evasion, even therapeutic outcomes. subtyping mainly using...