A5087571815- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Prostate Cancer Treatment and Research
- RNA Interference and Gene Delivery
- Metabolism, Diabetes, and Cancer
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- FOXO transcription factor regulation
- Viral Infectious Diseases and Gene Expression in Insects
- NF-κB Signaling Pathways
- Animal Virus Infections Studies
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Thyroid Cancer Diagnosis and Treatment
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Microtubule and mitosis dynamics
Universidade Federal de São Paulo
2018-2024
Instituto de Ciências Farmacêuticas
2023
Instituto do Câncer do Estado de São Paulo
2013-2022
Universidade de São Paulo
2008-2022
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2021
International Centre for Genetic Engineering and Biotechnology
2010-2016
University of Cape Town
2011-2016
The S6 Kinase (S6K) proteins are some of the main downstream effectors mammalian Target Of Rapamycin (mTOR) and act as key regulators protein synthesis cell growth. S6K is overexpressed in a variety human tumors correlated to poor prognosis prostate cancer. Due current urgency identify factors involved cancer progression, we aimed reveal cellular functions three isoforms–p70-S6K1, p85-S6K1 p54-S6K2–in cancer, well their potential therapeutic targets. In this study performed knockdown...
Several epidemiological studies have correlated the use of non-steroidal anti-inflammatory drugs (NSAID) with reduced risk ovarian cancer, most lethal gynecological diagnosed usually in late stages disease. We previously established that pro-apoptotic cytokine melanoma differentiation associated gene-7/Interleukin-24 (mda-7/IL-24) is a crucial mediator NSAID-induced apoptosis prostate, breast, renal and stomach cancer cells. In this report we evaluated various structurally different NSAIDs...
COVID-19 has stroke Brazil harshly, deaths by in represent almost 13% of the total world, even though Brazilian population represents only 2.6% world population. Our aim this study was to evaluate death and intubation outcomes risk factors associated with COVID-19, treatment options focusing on diabetes patients use metformin pre-admission during hospitalization.
Characterization of Human Respiratory Syncytial Virus (HRSV) protein interactions with host cell components is crucial to devise antiviral strategies. Viral nucleoprotein, phosphoprotein and matrix genes were optimized for human codon usage cloned into expression vectors. HEK-293T cells transfected these vectors, viral proteins immunoprecipitated, co-immunoprecipitated cellular identified through mass spectrometry. Cell higher confidence scores probed in the immunoprecipitation using...
Vitamin D transcriptional effects were linked to tumor growth control, however, the hormone targets determined in cell cultures exposed supra physiological concentrations of 1,25(OH)(2)D(3) (50-100nM). Our aim was evaluate a more model breast cancer, consisting fresh slices at that can be attained vivo.Tumor samples from post-menopausal cancer patients sliced and cultured for 24 hours with or without 0.5nM 100nM. Gene expression analyzed by microarray (SAM paired analysis, FDR≤0.1) RT-qPCR...
Previously, the authors developed an adenoviral vector, Ad-PG, where transgene expression is regulated by a p53-responsive promoter. When used to transfer p53 cDNA, positive feedback mechanism established. In present study, critical comparison performed between Ad-PGp53 and AdRGD-PGp53, RGD motif was incorporated in fiber protein. AdRGD-PGp53 provided superior levels resulted killing of prostate carcinoma cell lines DU145 PC3. vitro, this effect associated with increased production...
Adenovirus was first identified in the 1950s and since then this pathogenic group of viruses has been explored transformed into a genetic transfer vehicle. Modification or deletion few genes are necessary to transform it conditionally non-replicative vector, creating versatile tool capable transducing different tissues inducing high levels transgene expression. In early years vector development, application monogenic diseases faced several hurdles, including short-term gene expression even...
Alternative treatments for cancer using gene therapy approaches have shown promising results and some even reached the marketplace. Even so, additional improvements are needed, such as employing a strategically chosen promoter to drive expression of transgene in target cell. Previously, we described viral vectors where high-level was achieved p53-responsive promoter. Here present an adenoviral vector (AdPGp53) p53 is employed regulate its own which outperforms traditional when tested model...
Aging | doi:10.18632/aging.203528. Tharcisio Citrangulo Tortelli, Rodrigo Esaki Tamura, Mara de Souza Junqueira, Janio da Silva Mororó, Silvina Odete Bustos, Renato Jose Mendonça Natalino, Shonagh Russell, Laurent Désaubry, Bryan Eric Strauss, Roger Chammas
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), as well SARS-CoV from 2003 along with MERS-CoV 2012, is a member of the Betacoronavirus genus Nidovirales order and currently cause pandemic called COVID-19 (or disease 2019). COVID-19, which characterized by cough, fever, fatigue, severe cases pneumonia, has affected more than 23 million people worldwide until August 25th, 2020. Here, we present review cellular mechanisms associated human coronavirus replication, including unique...
The epithelium-specific Ets transcription factor, SPDEF, plays a critical role in metastasis of prostate and breast cancer cells. While enhanced SPDEF expression blocks migration invasion, knockdown enhances migration, activation is tightly regulated cells; however, the precise mechanism regulation has not been explored detail. In this study we provide evidence that cell cycle kinase CDK11p58, protein involved G2/M transition degradation several factors, directly interacts with...
Abstract Human Papillomavirus is responsible for a wide range of mucosal lesions and tumors. The immune system participate in tumorigenesis different ways. For example, signaling pathways triggered by Toll-like receptors (TLR) play role chemotherapy resistance several tumor types are candidates contributing to the development HPV-induced Here, we studied receptor TLR4 adaptor molecule SARM1 HeLa cells, an HPV-positive cervical cancer cell line. Knocking out these genes individually proved be...
<i>Objectives:</i> To construct a recombinant baculovirus expressing the fiber knob domain of human adenovirus type 2 modified by insertion foreign peptide, purify this protein after its production in insect cells, and to test properties. <i>Methods:</i> Recombinant baculoviruses were produced <i>Sf</i>9 cells. The was recovered from culture supernatants infected cells purified combination Ni-NTA ion-exchange chromatography. <i>Results:</i>...