A5071147948- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Escherichia coli research studies
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Leptospirosis research and findings
- Antibiotics Pharmacokinetics and Efficacy
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- Cancer Mechanisms and Therapy
- Salmonella and Campylobacter epidemiology
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Microbial Natural Products and Biosynthesis
- Biochemical Acid Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Artificial Intelligence in Healthcare
- Digital Imaging for Blood Diseases
- Peptidase Inhibition and Analysis
- Mechanisms of cancer metastasis
Institute of Chemistry
2025
Universidade de São Paulo
2016-2024
The type VI secretion system (T6SS) secretes antibacterial effectors into target competitors. Salmonella spp. encode five phylogenetically distinct T6SSs. Here, we characterize the function of SPI-22 T6SS bongori showing that it has activity and identify a group (TseV1–4) containing an N-terminal PAAR-like domain C-terminal VRR-Nuc encoded next to cognate immunity proteins with DUF3396 (TsiV1–4). TseV2 TseV3 are toxic when expressed in Escherichia coli bacterial competition assays confirm...
Herein, we present a novel esterase enzyme, Ade1, isolated from metagenomic library of Amazonian dark earths soils, demonstrating its broad substrate promiscuity by hydrolyzing ester bonds linked to aliphatic groups. The three-dimensional structure the enzyme was solved in presence and absence (tributyrin), revealing classification within α/β-hydrolase superfamily. Despite being monomeric enzymatic assays reveal cooperative behavior with sigmoidal profile (initial velocities vs...
The Leptospiraceae family, which includes the genera Leptospira, Leptonema, and Turneriella, is an ecologically diverse group that saprophytic strains from soil water as well important pathogenic strains. Adaptation to these multiple environments relies strongly on signal transduction adjust their morphology, motility, metabolism changing environmental conditions. Members of genus Leptospira distinguish themselves among spirochetes for having elevated number genes. In this study, we describe...
Several Xanthomonas species have a type IV secretion system (T4SS) that injects cocktail of antibacterial proteins into neighbouring Gram-negative bacteria, often leading to rapid lysis upon cell contact. This capability represents an obvious fitness benefit since it can eliminate competition while the liberated contents lysed bacteria could provide increase in local availability nutrients. However, production this Mega Dalton-sized molecular machine, with over hundred subunits, also imposes...
Type IV pili (T4P) are thin and flexible filaments found on the surface of a wide range Gram-negative bacteria that undergo cycles extension retraction participate in variety important functions related to lifestyle, defense pathogenesis. During pilus extensions, PilB ATPase energizes polymerization pilin monomers from inner membrane. In Xanthomonas citri , two cytosolic proteins, PilZ c-di-GMP receptor FimX, involved regulation T4P biogenesis through interactions with PilB. vivo...
Summary Establishing new experimental animal models to assess the safety and immune response antigen used in development of COVID-19 vaccine is an imperative issue. Based on advantages using zebrafish as a model research, herein we suggest doing this test putative candidates study against virus. We produced recombinant N-terminal fraction Spike SARS-CoV-2 protein injected it into adult female zebrafish. The specimens generated humoral immunity passed antibodies eggs. However, they presented...
The class D β-lactamase OXA-143 has been described as an efficient penicillinase, oxacillinase, and carbapenemase. D224A variant, known OXA-231, was in 2012 exhibiting less activity toward imipenem increased oxacillinase activity. Additionally, the P227S mutation reported a case of convergent evolution for homologous enzymes. To investigate impact both mutations (D224A P227S), we describe this paper deep investigation enzymatic activities these three homologues. OXA-143(P227S) presented...
Abstract The T6SS (Type VI secretion System) secretes antibacterial effectors into target competitors. Salmonella spp. encode five phylogenetically distinct T6SSs. Here we characterize the function of SPI-22 S. bongori , showing that it has activity. We identify a group (TseV1-4) containing an N-terminal PAAR-like domain and C-terminal VRR-Nuc encoded next to cognate immunity proteins contain DUF3396 (TsiV1-4). TseV2 TseV3 are toxic when expressed in Escherichia coli bacterial competition...
Abstract The causative agent of citrus canker disease, Xanthomonas citri pv. , was found to produce copious amounts outer membrane vesicles (OMVs), frequently forming long membranous tubes under different culture conditions. Lipidomic analysis revealed significant differences in lipid composition between purified relation whole cells. results suggest an enrichment saturated cardiolipins and a decrease unsaturated lipids the OMV samples, possibly granting them more rigid structure while...