A5011500405- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Signaling Pathways in Disease
- Glycosylation and Glycoproteins Research
- Gut microbiota and health
- Plant-Microbe Interactions and Immunity
- Toxin Mechanisms and Immunotoxins
- Microbial Metabolic Engineering and Bioproduction
- Insect Resistance and Genetics
- Diet, Metabolism, and Disease
- Carbohydrate Chemistry and Synthesis
- Phytoplasmas and Hemiptera pathogens
- Biochemical and Structural Characterization
- Plant pathogens and resistance mechanisms
- Plant Virus Research Studies
- Fungal Biology and Applications
- Cocoa and Sweet Potato Agronomy
- Infant Nutrition and Health
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Food composition and properties
- Plant Reproductive Biology
Brazilian Center for Research in Energy and Materials
2010-2022
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2010-2021
Brazilian Biosciences National Laboratory
2010-2015
Piedmont International University
2013
Brazilian Synchrotron Light Laboratory
2013
Universidade Estadual de Campinas (UNICAMP)
2011
Fundação de Amparo à Pesquisa do Estado de São Paulo
2011
The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the community composition, enzymatic systems metabolic pathways involved in conversion of dietary fibers into short-chain fatty acids, a main energy source for host. In this microbiota, unconventional machinery from Fibrobacteres seems to drive cellulose degradation, whereas diverse set carbohydrate-active enzymes...
Transcriptional activator-like (TAL) effectors, formerly known as the AvrBs3/PthA protein family, are DNA-binding effectors broadly found in Xanthomonas spp. that transactivate host genes upon injection via bacterial type three-secretion system. Biologically relevant targets of TAL i.e. whose induction is vital to establish a compatible interaction, have been reported for xanthomonads colonize rice and pepper; however, citrus modulated by PthA"s" PthC"s" canker bacteria citri (Xc)...
Abstract Xylanolytic enzymes from glycoside hydrolase family 43 (GH43) are involved in the breakdown of hemicellulose, second most abundant carbohydrate plants. Here, we kinetically and mechanistically describe non-reducing-end xylose-releasing exo-oligoxylanase activity report crystal structure a native GH43 Michaelis complex with its substrate prior to hydrolysis. Two distinct calcium-stabilized conformations active site xylosyl unit found, suggesting two alternative catalytic routes....
Xanthomonas axonopodis pv. citri utilizes the type III effector protein PthA to modulate host transcription promote citrus canker. proteins belong AvrBs3/PthA family and carry a domain comprising tandem repeats of 34 amino acids that mediates protein-protein protein-DNA interactions. We show here variants PthAs from single bacterial strain localize nucleus plant cells form homo- heterodimers through association their repeat regions. hypothesize might also interact with distinct targets....
Many plant pathogenic bacteria rely on effector proteins to suppress defense and manipulate host cell mechanisms cause disease. The protein PthA modulates the transcriptome promote citrus canker. possesses unusual architecture with an internal region encompassing variable numbers of near-identical tandem repeats 34 amino acids termed repeat domain. This domain mediates protein-protein protein-DNA interactions, two polymorphic residues in each unit determine DNA specificity. To gain insights...
Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs). In this work, we identified a novel GH51 Abf (XacAbf51) forms trimers solution can cope efficiently with both mono- at terminal or internal xylopyranosyl units of arabinoxylan. Using mass spectrometry, kinetic parameters hydrolysis...
The citrus (Citrus sinensis) cyclophilin CsCyp is a target of the Xanthomonas citri transcription activator-like effector PthA, required to elicit cankers on citrus. binds thioredoxin CsTdx and carboxyl-terminal domain RNA polymerase II divergent that carries additional loop KSGKPLH, invariable cysteine (Cys) residues Cys-40 Cys-168, conserved glutamate (Glu) Glu-83. Despite suggested roles in ATP metal binding, functions these unique structural elements remain unknown. Here, we show Cys...
Transcriptional activator-like (TAL) effectors of plant pathogenic bacteria function as transcription factors in cells. However, how TAL control the host is presently unknown. Previously, we showed that citrus canker pathogen Xanthomonas citri, named PthAs, targeted protein complex comprising thioredoxin CsTdx, ubiquitin-conjugating enzymes CsUev/Ubc13 and cyclophilin CsCyp. Here show CsCyp complements Cpr1 Ess1, two yeast cyclophilins regulate by isomerization proline residues regulatory...
Abstract Rational design is an important tool for sculpting functional and stability properties of proteins its potential can be much magnified when combined with in vitro natural evolutionary diversity. Herein, we report the structure‐guided a xylose‐releasing exo‐β‐1,4‐xylanase from inactive member glycoside hydrolase family 43 (GH43). Structural analysis revealed nonconserved substitution (Lys 247 ) that results disruption hydrogen bond network supports catalysis. The mutation this...
The glycoside hydrolase family 39 (GH39) is a functionally expanding with limited understanding about the molecular basis for substrate specificity and extremophilicity. In this work, we demonstrate key role of positive-subsite region in modulating affinity how lack C-terminal extension impacts on oligomerization structural stability some GH39 members. crystallographic SAXS structures new member from phytopathogen Xanthomonas citri support importance an extended to promote as strategy...
Transcriptional activator-like (TAL) effectors of plant pathogenic bacteria function as transcription factors in cells.However, how TAL control the host is presently unknown.Previously, we showed that citrus canker pathogen Xanthomonas citri, named PthAs, targeted protein complex comprising thioredoxin CsTdx, ubiquitin-conjugating enzymes CsUev/Ubc13 and cyclophilin CsCyp.Here show CsCyp complements Cpr1 Ess1, two yeast cyclophilins regulate by isomerization proline residues regulatory...
Abstract Background: Plant biomass is a promising feedstock to replace fossil-based products including fuels, chemicals and materials. However, the high resistance of plant either physicochemical or biological deconstruction has been hampering its broad industrial utilization and, consequently, transition sustainable bioeconomy. The gut system from herbivores are formidable bioreactors in nature for lignocellulose breakdown diverse ecological niches where found have led rise myriad molecular...