A5035058719- Enzyme-mediated dye degradation
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Sugarcane Cultivation and Processing
- Enzyme Production and Characterization
- Advanced Cellulose Research Studies
- Food composition and properties
- Catalysis for Biomass Conversion
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Pesticide and Herbicide Environmental Studies
- Fungal Biology and Applications
- Microbial bioremediation and biosurfactants
- Fermentation and Sensory Analysis
- Polysaccharides and Plant Cell Walls
- Wood Treatment and Properties
- Enzyme Catalysis and Immobilization
- Chromium effects and bioremediation
- Polymer-Based Agricultural Enhancements
- Particle physics theoretical and experimental studies
- Wood and Agarwood Research
- High-Energy Particle Collisions Research
- Nanoparticles: synthesis and applications
- Natural Products and Biological Research
Universidade de São Paulo
2015-2025
Polytechnic Institute of Porto
2020-2023
Universidade do Porto
2017
Biotech (Brazil)
1992-2014
Departamento de Ciência e Tecnologia
1997-2005
Universidade Federal do Paraná
1999
North Carolina State University
1999
Universidade Estadual de Campinas (UNICAMP)
1990-1995
LIP - Laboratory of Instrumentation and Experimental Particle Physics
1989
Pontificia Universidad Católica de Valparaíso
1987
The recalcitrance of lignocellulosic materials is a major limitation for their conversion into fermentable sugars. Lignin depletion in new cultivars or transgenic plants has been identified as way to diminish this recalcitrance. In study, we assessed the success sugarcane breeding program selecting with low lignin content, and report chemical composition agronomic characteristics eleven experimental hybrids two reference samples. enzymatic digestion untreated chemically delignified samples...
Lignin and hemicelluloses are the major components limiting enzyme infiltration into cell walls. Determination of topochemical distribution lignin aromatics in sugar cane might provide important data on recalcitrance specific cells. We used cellular ultraviolet (UV) microspectrophotometry (UMSP) to topochemically detect hydroxycinnamic acids individual fiber, vessel parenchyma walls untreated chlorite-treated cane. Internodes, presenting typical vascular bundles sucrose-storing cells, were...
The effectiveness of the enzymatic hydrolysis cellulose in plant cell wall is strongly influenced by access enzymes to cellulose, which at least part limited presence lignin. Although physicochemical treatments preceding catalysis significantly overcome this recalcitrance, residual lignin can still play a role process. Lignin suggested act as barrier, hindering and limiting enzymes. It also unspecifically bind cellulases, reducing amount available on cellulose. However, present pretreated...
Waste materials from agroindustry constituted lignocellulose have been used in the immobilization process of technological interest enzymes to reduce costs, increasing value such products. In present study, green coconut husk was obtain fibers (CF) that were treated through thermal decompression combination with either acid or alkaline medium. A solid support for enzyme prepared using pretreated CF activated glyoxyl glutaraldehyde and immobilize laccase (EC 1.10.3.2) produced by Trametes...
This work evaluated sugarcane bagasse pretreatment with wood-decay fungi, producing varied patterns of biodegradation. The overall mass balance sugars released after and enzymatic hydrolysis indicated that a selective white-rot was necessary to provide glucose yields similar the ones observed from leading physico-chemical technologies. Ceriporiopsis subvermispora for lignin degradation in lignocellulosic material, preserved most glucan fraction, increased cellulose digestibility biotreated...
New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis the glucan component is a way add value hemicellulose fraction, which can be used in papermaking, pharmaceutical, and food industries. Hemicellulose removal also facilitate subsequent cellulolytic hydrolysis. Sugarcane bagasse was pretreated with an alkaline-sulfite chemithermomechanical process extraction xylan by or alkaline procedures. Alkaline...
This study examines the effect of surfactant-enhanced enzymatic hydrolysis on eucalyptus Kraft pulps produced under high (CPHA) and mild (CPMA) alkali conditions to optimize saccharification sugar yield. Compositional analysis revealed an increase in glucan content, from 40% untreated 70.1% CPHA. Both were hydrolyzed using Cellic® CTec3 HS enzyme (Novozymes). A 22 factorial design maximum conversion (~100%) with loading 10 FPU/g carbohydrate 10% (w/v) solids. Tween 20 significantly boosted...
ABSTRACT Pinus taeda wood chips were biotreated with Ceriporiopsis subvermispora under solid-state fermentation for periods varying from 15 to 90 days. Milled lignins extracted sound and samples characterized by wet-chemical spectroscopic techniques. Treatment of the derivatization followed reductive cleavage (DFRC) made it possible detect DFRC monomers dimers that are diagnostic occurrence arylglycerol-β- O -aryl β-β, β-5, β-1, 4- -5 units in lignin structure. Quantification these products...
Abstract Chemithermomechanical (CTM) processing was used to pretreat sugarcane bagasse with the aim of increasing cell wall accessibility hydrolytic enzymes. Yields pretreated samples were in range 75–94%. Disk refining and alkaline‐CTM alkaline/sulfite‐CTM pretreatments yielded materials 21.7, 17.8, 15.3% lignin, respectively. Hemicellulose content also decreased some extent. Fibers presented external fibrillation, fiber curling, increased swelling, high water retention capacity. Cellulose...
Glycoside hydrolases (GHs) and accessory proteins are key components for efficient cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. Currently, commercialized GHs produced by ascomycetes. However, the role wood decay basidiomycetes biomass saccharification has not been extensively pursued. Wood fungi degrade highly lignified tissues natural environments, a promising enzyme source improving cocktails that designed vitro lignocellulose...