A5004247456- Trypanosoma species research and implications
- Sleep and Wakefulness Research
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Research on Leishmaniasis Studies
- Fungal Infections and Studies
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Bioinformatics and Genomic Networks
- Biochemical and Molecular Research
- Gene Regulatory Network Analysis
- Evolution and Genetic Dynamics
- Mitochondrial Function and Pathology
- Bacteriophages and microbial interactions
- Forensic and Genetic Research
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- Plant Pathogens and Fungal Diseases
- Parasites and Host Interactions
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Machine Learning in Bioinformatics
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
Universidade Federal de São Paulo
2015-2025
Pennsylvania State University
2017-2018
Institute of Ecology and Biodiversity
2018
University of Chile
2018
Universidade de São Paulo
1992-2017
Union des Industries Ferroviaires Européennes
2016
Associação Paulista de Medicina
1993-2008
Universidade Estadual de Campinas (UNICAMP)
2000-2001
Columbia University Irving Medical Center
1996
New York University
1996
In an effort to unify the nomenclature of Trypanosoma cruzi, causative agent Chagas disease, updated system was agreed upon at Second Satellite Meeting.A consensus reached that T. cruzi strains should be referred by six discrete typing units (T.cruzi I-VI).The goal a unified is improve communication within scientific community involved in research.The justification and implications will presented subsequent detailed report.
Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections creating new risks for established ones. There is need rapid accurate identification of enable early diagnosis targeted antifungal therapy. Morphological biochemical methods time-consuming require trained experts. Alternatively, molecular methods, such as DNA barcoding, powerful easy tool monophasic identification, offer practical approach species less demanding in terms taxonomical expertise....
Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential central portion transcript. Systematic, high-throughput sequencing such products would an sequence tag (EST) database consisting central, generally coding regions genes. Such a add significant value to existing public EST databases, which consist mostly sequences derived from extremities cDNAs, and facilitate...
We present the sequencing and annotation of Leishmania (Leishmania) amazonensis genome, an etiological agent human cutaneous leishmaniasis in Amazon region Brazil. L. (L.) shares features with mexicana but also exhibits unique characteristics regarding geographical distribution clinical manifestations lesions (e.g. borderline disseminated leishmaniasis). Predicted genes were scored for orthologous gene families conserved domains comparison other pathogenic spp. Carboxypeptidase,...
Genetic variation in the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region has been studied among fungi. However, numbers of ITS sequence polymorphisms various Candida species and their associations with sources invasive fungal infections remain poorly investigated. Here, we characterized intraspecific interspecific diversity spp. strains collected from patients bloodstream or oroesophageal candidiasis. We selected cultures representative medically important as well some rare...
ABSTRACT Proteasomes degrade most of the proteins inside eukaryotic cells, including transcription factors and regulators cell cycle progression. Here we show that nanomolar concentrations lactacystin, a specific irreversible inhibitor 20S proteasome, inhibit development exoerythrocytic erythrocytic stages malaria parasite. Although lactacystin-treated Plasmodium berghei sporozoites are still invasive, their into forms (EEF) is inhibited in vitro vivo. Erythrocytic schizogony P. falciparum...
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data the central protein coding portion of transcripts. We generated a total 696,745 ORESTES 24 human tissues and used subset that correspond to set 15,095 full-length mRNAs as means assessing efficiency strategy its potential contribution definition transcriptome. estimate sampled over 80% all highly moderately expressed, between 40% 50% rarely genes. In our most thoroughly sequenced...
Remnants of lipoproteins, intestinal chylomicrons, and very low density lipoprotein (VLDL), are rapidly cleared from plasma enter hepatocytes. It has been suggested that remnant lipoproteins initially captured in the space Disse by heparan sulfate proteoglycans (HSPGs), their subsequent internalization into hepatocytes is mediated members LDL-receptor gene family. Similarly to remnants, malaria sporozoites removed blood circulation liver within minutes after injection Anopheles mosquitoes....
Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence codes a 21kDa protein selected. After cloning this sequence, the results it ubiquitous by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells dose-dependent manner. Pretreatment host with P21-His(6) inhibited cell invasion amastigotes from G CL strains. On other...
Enolase is secreted by C. albicans and present in its biofilms although extracellular function unknown. Here we show that enolase mediates the colonization of small intestine mucosa albicans. Assays using intestinal disks adhesion inhibited, a dose dependent mode, either pretreatment epithelium with recombinant (70% at 0.5 mg/ml enolase) or yeasts anti-enolase antibodies (48% 20 µg antiserum). Also flow cytometry, immunoblots conditioned media confocal microscopy demonstrate not an artifact...
The causative agent of COVID-19 pandemic, SARS-CoV-2, has a 29,903 bases positive-sense single-stranded RNA genome. RNAs exhibit about 150 modified that are essential for proper function. Among internal bases, the N6-methyladenosine, or m6A, is most frequent, and implicated in SARS-CoV-2 immune response evasion. Although genome RNA, almost all genomes sequenced thus far are, fact, reverse transcribed complementary DNAs. This process reduces true complexity these viral because incorporation...
Ancient human viruses have been detected in ancient DNA (aDNA) samples of both Anatomically Modern Humans and Neanderthals. Reconstructing genomes from aDNA using reference mapping presents numerous problems due to the unique nature samples, their degraded state, smaller read sizes limitations current methodologies. The spurious alignments reads sequences (mapping) are a main source false positives assemblies assessment signal-to-noise ratios is essential differentiate bona fide...
Transcribed sequences in the human genome can be identified with confidence only by alignment derived from cDNAs synthesized naturally occurring mRNAs. We constructed a set of 250,000 that represent partial expressed gene and are biased toward central coding regions resulting transcripts. They termed ORF sequence tags (ORESTES). The ORESTES were assembled into 81,429 contigs. Of these, 1,181 (1.45%) found to match chromosome 22 at least one contig for 162 (65.6%) 247 known genes, 67 (44.6%)...
The phylogenetic relationships of Trypanosoma cruzi strains were inferred using maximum-likelihood from complete 18S rDNA sequences and D7-24Salpha regions 20 representative T. cruzi. For this we sequenced the 14 four aligned them to previously published sequences. Phylogenies these data sets identified groups, named Riboclades 1, 2, 3, 4, a basal dichotomy that separated Riboclade 1 4. Substitution models other parameters optimized by hierarchical likelihood tests, our analysis molecular...
Enteropathogenic Escherichia coli (EPEC) forms attaching and effacing lesions in the intestinal mucosa characterized by intimate attachment to epithelium means of intimin (an outer membrane adhesin encoded eae). EPEC is subgrouped into typical (tEPEC) atypical (aEPEC); only tEPEC carries EAF (EPEC adherence factor) plasmid that encodes bundle-forming pilus (BFP). Characteristically, after 3 h incubation, produces localized (LA) (with compact microcolonies) HeLa/HEp-2 cells BFP, whereas most...
Sporozoites of P. yoelii nigeriensis are 50–100-times more infective to mice than the strain NK65 berghei. To study mechanisms involved in this striking difference infectivity these closely related species malaria parasites, we have developed a quantitative PCR targeted parasite-specific ribosomal RNA. Using method, detect RNA from single sporozoite, and exo-erythorcytic forms livers injected with 200 sporozoites. We find that 20 h after sporozoite injection, there is no significant between...
OPINION article Front. Genet., 11 July 2013 | https://doi.org/10.3389/fgene.2013.00130