A5086391123- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Gastroesophageal reflux and treatments
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Blood Coagulation and Thrombosis Mechanisms
- Parasites and Host Interactions
- Liver Disease Diagnosis and Treatment
- Synthesis and Biological Evaluation
- Diet and metabolism studies
- Neuropeptides and Animal Physiology
- Growth Hormone and Insulin-like Growth Factors
- Toxin Mechanisms and Immunotoxins
- Hormonal Regulation and Hypertension
- Insect and Pesticide Research
- Receptor Mechanisms and Signaling
- Diet, Metabolism, and Disease
- Hormonal and reproductive studies
- Pituitary Gland Disorders and Treatments
- Stress Responses and Cortisol
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Neuroendocrine regulation and behavior
- Phytochemistry Medicinal Plant Applications
- Lysosomal Storage Disorders Research
Universidade Federal de São Paulo
2010-2025
Instituto de Infectologia Emílio Ribas
2013
Instituto de Medicina Avançada
2013
Universidade de São Paulo
2013
Universidade Federal de Goiás
2007
Fundação Oswaldo Cruz
2007
Universidade Federal da Bahia
2007
Universidade Estadual de Campinas (UNICAMP)
2003
Fundação de Apoio à Universidade Federal de São Paulo
1997
Institute of Parasitology
1997
We present the sequencing and annotation of Leishmania (Leishmania) amazonensis genome, an etiological agent human cutaneous leishmaniasis in Amazon region Brazil. L. (L.) shares features with mexicana but also exhibits unique characteristics regarding geographical distribution clinical manifestations lesions (e.g. borderline disseminated leishmaniasis). Predicted genes were scored for orthologous gene families conserved domains comparison other pathogenic spp. Carboxypeptidase,...
The new long-acting ergoline derivative cabergoline was given orally in a single dose of 300 micrograms to 15 hyperprolactinemic patients (including 4 acromegalic patients, 2 whom were dopamine responsive). Serum PRL and GH levels determined before 3, 4, 6 h 1, 2, 5, 6, 7 days after treatment. A control test with oral 2.5 mg bromocriptine also performed; serum measured at the same time intervals for days. Cabergoline induced marked fall which began within 3 continued maximal decrease ranged...
Infections by protozoans of the genus Leishmania are major worldwide health problem, with high endemicity in developing countries. The drugs choice for treatment leishmaniasis pentavalent antimonials, which exert renal and cardiac toxicity. Thus, there is a strong need safer more effective treatments against leishmaniasis. present study was designated to evaluate, bioguided assay, leishmanicidal activity extracts (hexane, ethyl-acetate ethanolic) molecules both obtained means extraction from...
The aim of this study was to evaluate the efficacy tamoxifen in vivo experimental models cutaneous (CL) and visceral leishmaniasis (VL) caused by Leishmania braziliensis chagasi, respectively.Drug activity assessed against intracellular amastigotes treating infected macrophage cultures evaluating number cells. In tested L. braziliensis-infected BALB/c mice chagasi-infected hamsters. Treatment with 20 mg/kg/day administered for 15 days intraperitoneal route. Efficacy evaluated through...
Background Studies on the role of neutrophils in Leishmania infection were mainly performed with L. (L) major, whereas less information is available for amazonensis. Previous results from our laboratory showed a large infiltrate site mouse strain resistant to (L.) amazonensis (C3H/HePas). In contrast, susceptible (BALB/c) displayed predominance macrophages harboring high number amastigotes and very few neutrophils. These findings led us investigate interaction inflammatory...
Background Antitumor cyclopalladated complexes with low toxicity to laboratory animals have shown leishmanicidal effect. These findings stimulated us test the property of one palladacycle compound called DPPE 1.2 on Leishmania (Leishmania) amazonensis, an agent simple and diffuse forms cutaneous leishmaniasis in Amazon region, Brazil. Methodology/Principal Findings Promastigotes L. (L.) amazonensis infected bone marrow-derived macrophages were treated different concentrations 1.2. In vivo...
ABSTRACT Leishmania presents a complex life cycle that involves both invertebrate and vertebrate hosts. By regulating gene expression, protein synthesis, metabolism, the parasite can adapt to various environmental conditions. This regulation occurs mainly at post‐transcriptional level may involve epitranscriptomic modifications of RNAs. Recent studies have shown mRNAs in humans undergo modification known as N4‐acetylcytidine (ac4C) catalyzed by enzyme N‐acetyltransferase (NAT10), impacting...
Monoclonal antibodies directed against Leishmania (Leishmania) amazonensis amastigotes were produced. One monoclonal antibody (1C3) selected by indirect immunofluorescence reacted with both and promastigotes of L. (L.) amazonensis. Glycolipid extraction from separation high-performance thin-layer chromatography followed immunoblotting demonstrated that 1C3 reacts two glycosphingolipids which migrate chromatographically similarly to ceramide-N-acetylneuraminic acid (GM1)...
Tellurium compounds have shown several biological properties and recently the leishmanicidal effect of one organotellurane was demonstrated. These findings led us to test organotellurium compound RF07 on Leishmania (Leishmania) chagasi, agent visceral leishmaniasis in Latin America. In vitro assays were performed L. (L.) chagasi-infected bone marrow derived macrophages treated with different concentrations RF07. vivo experiments Golden hamsters infected chagasi injected intraperitoneally...
Journal Article Abnormal Alanine Aminotransferase Activity Reflects Exposure to Hepatitis C Virus in Haemodialysis Patients Get access M. U. Mondelli, Mondelli 1Istituto di Clinica delle Malattie Infettive, I.R.C.C.S. Policlinico San MatteoPavia Correspondence and offprint requests to: Mario MD, PhD, Istituto Matteo, viale Taramelli 5, 27100 Pavia, Italy. Search for other works by this author on: Oxford Academic PubMed Google Scholar V. Smedile, Smedile 2Istituto Medicina Interna, University...
ABSTRACT We evaluated whether four recombinant antigens previously used for vaccination against experimental infection with Leishmania ( ) major could also induce protective immunity a challenge Viannia braziliensis , the species responsible 90% of 28,712 annual cases cutaneous and mucocutaneous leishmaniasis recorded in Brazil during year 2004. Initially, we isolated homolog genes encoding L . V .) antigens: (i) homologue receptor activated C kinase, (ii) thiol-specific antioxidant, (iii)...
ABSTRACT In order to verify the applicability of biochemical methods for species identification Trypanosomatidae, 13 monoxenic trypanosomatids plus heteroxenous Trypanosoma cruzi were comparatively analyzed by three different methods. Insect examined were: Crithidia acanthocephali, C. fasciculata (three varieties), luciliae luciliae, thermophila, deanei, oncopelti, Herpetomonas muscarum muscarum, H. megaseliae, samuelpessoai, mariadeanei, Leptomonas seymouri, L. collosoma, samueli, and...
The infections by protozoans of the genus Leishmania are a major worldwide health problem, with high endemicity in developing countries. drugs choice for treatment leishmaniasis pentavalent antimonials, which cause renal and cardiac toxicity. As part search new against leishmaniasis, we evaluated vitro protease inhibition activity extracts (hexanic, ethyl-acetate, ethanolic) fukugetin, bioflavonoid purified from ethyl-acetate extract pericarp fruit Garcinia brasiliensis, tree native to...
The effects of chronic (3 mg/day for 1 week) administration the vasodilator drug prazosin on several metabolic and endocrine variables were evaluated in 12 hypertensive patients, 6 with normal abnormal oral glucose tolerance test (OGTT). After week treatment there no significant modifications fasting plasma glucose, serum free fatty acids (FFA), cholesterol, triglycerides, insulin (IRI), growth hormone (GH), prolactin (PRL) gastrin levels; IRI response to unchanged subjects, while chemical...
Background A recombinant cysteine proteinase from Leishmania (Leishmania) infantum chagasi (rLdccys1) was previously shown to induce protective immune responses against murine and canine visceral leishmaniasis. These findings encouraged us use rLdccys1 in the immunotherapy of naturally infected dogs Teresina, Piauí, a region high incidence leishmaniasis Brazil. Methodology/Principal Findings Thirty mongrel displaying clinical signs were randomly divided three groups: one group received doses...
Lymphoproliferative responses to an antigen from Leishmania amazonensis amastigotes with apparent molecular mass of 30 kDa, termed p30, were evaluated BALB/c mice. The p30 was purified after separation parasite extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed electroelution. obtained subcutaneous immunization animals L. amastigote extracts, and maximal stimulation indices observed at concentration 5 microg/ml. Induction lymphoproliferation is stage specific, no...
Antimony(Sb)-yeast mannan complexes were synthesized as a strategy to introduce Sb into macrophages infected with Leishmania amastigotes. The taken up by endocytosis after specific recognition alpha-D-mannosyl receptors on the macrophage membrane. About 90% of intracellular parasites destroyed Sb-mannan in vitro, whereas corresponding concentration used pentavalent antimonial drug glucantime about 60% None prepared acid or basic derivatives was effective simple complex clearing infection (L)...
A recombinant protein, rLdccys1, which was produced by expression of the gene encoding a 30 kDa cysteine proteinase from Leishmania (Leishmania) chagasi, used for detection antibodies in sera patients with active visceral leishmaniasis (VL) enzyme-linked immunosorbent assays. Analysis predicted amino acid sequence rLdccys1 showed that it contains all characteristics proteinase. The ability protein to react humans VL also shown Western blotting. sensitivity specific L. (L.) chagasi bodies...