A5042774820- Aquaculture disease management and microbiota
- Cell death mechanisms and regulation
- Vibrio bacteria research studies
- interferon and immune responses
- T-cell and B-cell Immunology
- Aquaculture Nutrition and Growth
- Genomics and Phylogenetic Studies
- Fish Biology and Ecology Studies
- Photodynamic Therapy Research Studies
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- bioluminescence and chemiluminescence research
- Bacterial Genetics and Biotechnology
- Nanoplatforms for cancer theranostics
- Toxin Mechanisms and Immunotoxins
- Autophagy in Disease and Therapy
- Yersinia bacterium, plague, ectoparasites research
- Coral and Marine Ecosystems Studies
- Bacterial biofilms and quorum sensing
- Drug Transport and Resistance Mechanisms
- Reproductive biology and impacts on aquatic species
- Phagocytosis and Immune Regulation
- Microbial infections and disease research
- Biosensors and Analytical Detection
Universidade do Porto
2010-2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2017-2025
Fundação de Apoio à Universidade Federal de São Paulo
2022
Instituto de Biologia Molecular e Celular
2002-2018
Universidade Federal de São Paulo
2002-2007
Universidade Federal de Goiás
2007
Fundação Oswaldo Cruz
2007
Universidade Federal da Bahia
2007
Institut de Biologie Moléculaire et Cellulaire
2005
Instituto de Agroquímica y Tecnología de Alimentos
2001
Interleukine-1β (IL-1β) is the most studied pro-inflammatory cytokine, playing a central role in generation of systemic and local responses to infection, injury, immunological challenges. In mammals, IL-1β synthesized as an inactive 31 kDa precursor that cleaved by caspase-1 generating 17.5 secreted active mature form. The cleavage site strictly conserved all mammalian sequences absent reported for non-mammalian vertebrates. Recently, fish orthologues have been identified sea bass...
The AB-type toxin AIP56 is a key virulence factor of Photobacterium damselae subsp. piscicida (Phdp), inducing apoptosis in fish immune cells. discovery AIP56-like and AIP56-related toxins diverse organisms, including human-associated Vibrio strains, highlights the evolutionary conservation this family, suggesting that its homologs may share conserved receptors across species. These have potential for biotechnological applications, such as therapeutic protein delivery modulation. Herein,...
Background/Objectives: The intracellular delivery of biologics, particularly large cargoes like proteins, remains a challenge in biotechnology and biomedicine. modular structure well-characterized AB toxins allows different to be grafted, creating target-specific biotechnological tool capable cytosolic delivery. Methods: In this study, we employed protein-protein fusion strategies - SpyCatcher003, SnoopCatcher, SnoopLigase generate chimeras between the region AIP56 (AIP56L258-N497)...
A strategy used by extracellular pathogens to evade phagocytosis is the utilization of exotoxins that kill host phagocytes. We have recently shown one major pathogenicity Photobacterium damselae subsp. piscicida (Phdp), agent widespread fish pasteurellosis, induction extensive apoptosis sea bass macrophages and neutrophils results in lysis these phagocytes post-apoptotic secondary necrosis. Here we show this unique process mediated a novel plasmid-encoded inducing protein 56 kDa (AIP56), an...
AIP56 (apoptosis-inducing protein of 56 kDa) is a major virulence factor Photobacterium damselae piscicida (Phdp), Gram-negative pathogen that causes septicemic infections, which are among the most threatening diseases in mariculture. The toxin triggers apoptosis host macrophages and neutrophils through process that, vivo, culminates with secondary necrosis apoptotic cells contributing to necrotic lesions observed diseased animals. Here, we show NF-κB p65-cleaving zinc-metalloprotease whose...
The foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible animals and humans. To survive proliferate within hosts, this facultative intracellular tightly coordinates the expression of a complex regulatory network that controls virulence factors. Here, we identified characterized MouR, novel regulator Lm. Through RNA-seq transcriptomic analysis, determined MouR regulon demonstrated how positively Agr quorum sensing system (agrBDCA) three-dimensional structure...
Mycobacteriosis (piscine tuberculosis) has been reported to affect a wide range of freshwater and marine fish species; however, this is the first report describing mycobacterial infections in turbot Scophthalmus maximus. High numbers granulomas were initially observed organs moribund farmed turbot. Bacteriological analysis with led isolation Mycobacterium marinum. Further analysis, determine prevalence infection farm identify its source, showed occurrence dual by M. marinum chelonae. The...
Bacterial modulation of phagocyte cell death is an emerging theme in pathogenesis. Here we describe the systemic destruction macrophages and neutrophils by Gram-negative Photobacterium damselae ssp. piscicida (Phdp) fish pasteurellosis, a deadly infection. Following experimental inoculation, Phdp spreads bacteraemia colonizes organs, producing septicaemic infection, secretes apoptogenic exotoxin AIP56 which systemically disseminated. In natural secondary necrosis following...
ABSTRACT We evaluated whether four recombinant antigens previously used for vaccination against experimental infection with Leishmania ( ) major could also induce protective immunity a challenge Viannia braziliensis , the species responsible 90% of 28,712 annual cases cutaneous and mucocutaneous leishmaniasis recorded in Brazil during year 2004. Initially, we isolated homolog genes encoding L . V .) antigens: (i) homologue receptor activated C kinase, (ii) thiol-specific antioxidant, (iii)...
AIP56 (apoptosis-inducing protein of 56 kDa) is a key virulence factor Photobacterium damselae subsp. piscicida (Phdp), the causative agent septicaemia affecting warm water marine fish species. Phdp-associated pathology triggered by AIP56, short trip AB toxin with metalloprotease A domain that cleaves p65 subunit NF-κB, an evolutionarily conserved transcription regulates expression inflammatory and anti-apoptotic genes plays central role in host responses to infection. During infection Phdp,...
Photobacterium damselae subsp. piscicida (Phdp) is a Gram-negative fish pathogen with worldwide distribution and broad host specificity that causes heavy economic losses in aquaculture. Although Phdp was first identified more than 50 years ago, its pathogenicity mechanisms are not completely understood. In this work, we report secretes large amounts of outer membrane vesicles (OMVs) when cultured vitro during vivo infection. These OMVs were morphologically characterized the most abundant...
Photobacterium damselae subsp. piscicida is a fish pathogen which causes serious disease in commercial warmwater species. Because information on the initial stages of infection scarce, an investigation invasion ability this was undertaken utilizing epithelial cell line (epithelioma papillosum carpio, EPC), virulent capsulated strain P. (MT1415), avirulent non-capsulated (EPOY- 8803-II) and Escherichia coli HB101 as non-invasive control. found to be able adhere invade cells remain inside them...