A5062254983- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Metalloenzymes and iron-sulfur proteins
- S100 Proteins and Annexins
- Protein purification and stability
- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Biofuel production and bioconversion
- Polysaccharides and Plant Cell Walls
- Blood groups and transfusion
- Biopolymer Synthesis and Applications
- Protein Hydrolysis and Bioactive Peptides
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Cell death mechanisms and regulation
- Fermentation and Sensory Analysis
- Pomegranate: compositions and health benefits
- Nanofabrication and Lithography Techniques
- Environmental Chemistry and Analysis
- Redox biology and oxidative stress
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2023
Universidade do Porto
2011-2022
Instituto de Biologia Molecular e Celular
2000-2018
Chitosan (Ch) is a nontoxic and biocompatible polysaccharide extensively used in biomedical applications. Ch, as polycation, can be combined with anionic polymers by layer-by-layer (LbL) self-assembly, giving rise to multilayered complexed architectures. These structures tissue engineering strategies, drug delivery systems, or artificial matrices mimicking the extracellular microenvironment. In this work, Ch was poly(γ-glutamic acid) (γ-PGA). γ-PGA polyanion, which microbially produced,...
The nature of the immune responses associated with COVID-19 pathogenesis and disease severity, as well breadth vaccine coverage duration immunity, is still unclear. Given unpredictability for developing a severe/complicated disease, there an urgent need in field predictive biomarkers COVID-19. We have analyzed IgG Fc N-glycan traits 82 SARS-CoV-2+ unvaccinated patients, at diagnosis, by nano-LC-ESI-MS. determined impact glyco-variations induction NK cells activation, further evaluating...
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the deposition of amyloid β-peptide (A-Beta) in brain. Transthyretin (TTR) tetrameric protein about 55 kDa mainly produced liver and choroid plexus The known physiological functions TTR are transport thyroid hormone T4 retinol, through binding to retinol protein. has also been established as cryptic protease able cleave ApoA-I vitro. It described that involved preventing A-Beta fibrilization, both inhibiting disrupting...
Two-cysteine peroxiredoxins are ubiquitous peroxidases that play various functions in cells. In Leishmania and related trypanosomatids, which lack catalase selenium-glutathione peroxidases, the discovery of this family enzymes provided molecular basis for peroxide removal these organisms. report functional relevance one such enzymes, mitochondrial 2-Cys peroxiredoxin (mTXNPx), was investigated along infantum life cycle. mTXNPx null mutants (mtxnpx−) produced by a gene replacement strategy,...
AIP56 (apoptosis-inducing protein of 56 kDa) is a major virulence factor Photobacterium damselae piscicida (Phdp), Gram-negative pathogen that causes septicemic infections, which are among the most threatening diseases in mariculture. The toxin triggers apoptosis host macrophages and neutrophils through process that, vivo, culminates with secondary necrosis apoptotic cells contributing to necrotic lesions observed diseased animals. Here, we show NF-κB p65-cleaving zinc-metalloprotease whose...
Miscibility is an important issue in biopolymer blends for analysis of the behavior polymer pairs through detection phase separation and improvement mechanical physical properties blend. This study presents formulation a stable one-phase mixture collagen regenerated silk fibroin (RSF), with highest miscibility ratio between these two macromolecules, inducing electrostatic interactions, using salt ions. For this aim, ternary diagram was experimentally built mixtures, based on observations...
The first contact of tannins with the human body occurs in mouth, where some these are known to interact salivary proteins, particular proline-rich proteins (PRPs). These interactions important at a sensory level, especially for astringency development, but could also affect biological activities tannins. This study gathers information on relative affinity interaction, complex stoichiometry, and tannin molecular epitopes binding between families PRPs (bPRPs, gPRPs, aPRPs) three...
Abstract Background Arabidopsis thaliana transthyretin-like (TTL) protein is a potential substrate in the brassinosteroid signalling cascade, having role that moderates plant growth. Moreover, sequence homology revealed two domains similar to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (N-terminal domain) and 5-hydroxyisourate (5-HIU) hydrolase (C-terminal domain). TTL member of transthyretin-related family (TRP), which comprises number proteins with transthyretin...
The receptor for advanced glycation end products (RAGE) is a multiligand cell surface involved in various human diseases, as it binds to numerous molecules and proteins that modulate the activity of other proteins. Elucidating three-dimensional structure this therefore most important understanding its function during activation cellular signaling. major alternative splice product RAGE comprises extracellular region occurs soluble protein (sRAGE). Although structures sRAGE domains were...
The presence of an isoform glyceraldehyde-3-phosphate dehydrogenase (kmGAPDH1p) associated with the cell wall a flocculent strain Kluyveromyces marxianus was first report non-cytosolic localization glycolytic enzyme, but mechanism by which protein is transported to surface not known. To identify structural features that could account for multiple localizations protein, three-dimensional structure kmGAPDH1p determined x-ray crystallography and small angle scattering. crystallographic revealed...
Antimicrobial peptides are widely recognized as an excellent alternative to conventional antibiotics. MSI-78, a highly effective and broad spectrum AMP, is one of the most promising AMPs for clinical application. In this study, we have designed shorter derivatives MSI-78 with aim improving selectivity while maintaining antimicrobial activity. Shorter 17-mer were created by truncating at N- and/or C-termini, spanning sequence. Despite truncations made, found peptide, MSI-78(4-20)...
A new pathway of molinate mineralization has recently been described. Among the five members mixed culture able to promote such a process, Gulosibacter molinativorax ON4(T) observed initial breakdown herbicide into ethanethiol and azepane-1-carboxylate. In current study, gene encoding enzyme responsible for hydrolysis was identified heterologously expressed, resultant active protein purified characterized. Nucleotide sequence analysis revealed that encodes 465-amino-acid metal-dependent...
The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use GMO in food or feed, and countries have legislated on labelling systems indicate presence commercial products. To deal with increased number events address related regulations, alternative detection methods for inspection are required. In this work, a genosensor based Surface Plasmon Resonance under continuous flow was developed quantification soybean...