Jerko Štambuk
A5049317331- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Genetic Associations and Epidemiology
- Epigenetics and DNA Methylation
- Blood groups and transfusion
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Liver Disease Diagnosis and Treatment
- Inflammatory Bowel Disease
- Cancer Research and Treatments
- Advanced Proteomics Techniques and Applications
- Lipid metabolism and disorders
- Protein purification and stability
- Digestive system and related health
- Caveolin-1 and cellular processes
- Pain Mechanisms and Treatments
- IL-33, ST2, and ILC Pathways
- Pancreatitis Pathology and Treatment
- Celiac Disease Research and Management
Science Research Laboratory
2015-2024
Genos (Croatia)
2015-2024
Delft University of Technology
2023
University of Zagreb
2019
Universidad Autónoma de Madrid
2012
Nippon Dental University
2012
Universidade do Porto
2012
Bursa Uludağ Üni̇versi̇tesi̇
2012
Centro de Biología Molecular Severo Ochoa
2012
Objective Glycans attached to the Fc portion of IgG are important modulators effector functions. Interindividual differences in glycome composition large and they associate strongly with different inflammatory autoimmune diseases. IKZF1 , HLA–DQ2A/B BACH2 genetic loci that affect show pleiotropy systemic lupus erythematosus (SLE), indicating a potentially causative role aberrant glycosylation SLE. We undertook this multicenter case–control study determine whether SLE is associated altered...
Abstract Epigenetic alterations may provide important insights into gene-environment interaction in inflammatory bowel disease (IBD). Here we observe epigenome-wide DNA methylation differences 240 newly-diagnosed IBD cases and 190 controls. These include 439 differentially methylated positions (DMPs) 5 regions (DMRs), which study detail using whole genome bisulphite sequencing. We replicate the top DMP ( RPS6KA2 ) DMRs VMP1, ITGB2 TXK an independent cohort. Using paired genetic epigenetic...
Glycobiology is an underexplored research area in inflammatory bowel disease (IBD), and glycans are relevant to many etiological mechanisms described IBD. Alterations N-glycans attached the immunoglobulin G (IgG) Fc fragment can affect molecular structure immunological function. Recent genome-wide association studies reveal pleiotropy between IBD IgG glycosylation. This study aims explore glycan changes ulcerative colitis (UC) Crohn's (CD).IgG glycome composition patients with UC (n = 507),...
Alternative glycosylation has significant structural and functional consequences on IgG consequently also cancer immunosurveillance. Because of technological limitations, the effects highly heritable individual variations differences in dynamics changes colorectal were never investigated before.
Effector functions of immunoglobulin G (IgG) are regulated by the composition a glycan moiety, thus affecting activity immune system. Aberrant glycosylation IgG has been observed in many diseases, but little is understood about underlying mechanisms. We performed genome-wide association study N-glycosylation (
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Abstract In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker colorectal cancer (CRC). We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes. assessed predictive clinical algorithms compared to that also included glycan predictors. Decreased galactosylation, decreased sialylation (of fucosylated structures) increased bisecting GlcNAc structures were strongly associated all-cause (q <...
Plasma protein N-glycan profiling integrates information on enzymatic glycosylation, which is a highly controlled ubiquitous posttranslational modification. Here we investigate the ability of plasma N-glycome to predict incidence type 2 diabetes and cardiovascular diseases (CVDs; i.e., myocardial infarction stroke).Based prospective European Prospective Investigation Cancer (EPIC)-Potsdam cohort (n = 27,548), constructed case-cohorts including random subsample 2,500 participants all...
The nature of the immune responses associated with COVID-19 pathogenesis and disease severity, as well breadth vaccine coverage duration immunity, is still unclear. Given unpredictability for developing a severe/complicated disease, there an urgent need in field predictive biomarkers COVID-19. We have analyzed IgG Fc N-glycan traits 82 SARS-CoV-2+ unvaccinated patients, at diagnosis, by nano-LC-ESI-MS. determined impact glyco-variations induction NK cells activation, further evaluating...
Abstract Inflammatory bowel disease (IBD) is characterized by chronic inflammation in the gut. There growing evidence Crohn’s (CD) of existence a preclinical period immunological changes preceding symptom onset that starts years before diagnosis. Gaining insight into this phase will allow prediction and prevention. Analysis serum samples, up to 6 IBD diagnosis (from PREDICTS cohort), revealed identification unique glycosylation signature on circulating antibodies (IgGs) lower galactosylation...
Immunoglobulin G (IgG) glycosylation modulates antibody activity and represents a major source of heterogeneity within preparations. Depending on their pattern, individual IgG glycovariants present in recombinant preparations may trigger effects ranging from enhanced pro-inflammatory to increased anti-inflammatory activity. In contrast, reduction beyond the central mannose core is generally believed result impaired However, this study reveals that mono- or disaccharide structure consisting...
Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays major role in the human adaptive immune response and are associated to wide range of diseases. Glycosylation Fc binding region IgGs, responsible for antibody's effector function, is essential prompting proper response. This study focuses on general genetic impact IgG glycosylation as well corresponding subclass-specificities. To identify loci involved glycosylation, we performed genome-wide association (GWAS) liquid...
Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through attachment of various sugar moieties called glycans. Composition IgG N-glycome changes with age an individual in different diseases. Variability glycosylation within a population well studied known to be affected by both genetic environmental factors. However, global inter-population differences have never been properly addressed. Here we present...
Immunoglobulin G (IgG) is a major effector molecule of the human immune response, and aberrations in IgG glycosylation are linked to various diseases. However, molecular mechanisms underlying protein still poorly understood. We present data-driven approach infer reactions pathway using large-scale mass-spectrometry measurements. Gaussian graphical models used construct association networks from four cohorts. find that glycan pairs with high partial correlations represent enzymatic known...
Glycosylation is a common post-translational modification of proteins. associated with number human diseases. Defining genetic factors altering glycosylation may provide basis for novel approaches to diagnostic and pharmaceutical applications. Here we report genome-wide association study the blood plasma N-glycome composition in up 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology. Starting 36 original traits UPLC, computed an additional 77 derived leading...
Next-generation (postgenomic) biomarkers from the nascent field of glycomics now offer fresh vistas for innovation in chronic disease and system diagnostics clinical medicine. Our previous work has shown an association between hypertension immunoglobulin G (IgG) glycome composition, suggesting that individual variation N-glycosylation IgG might contribute to pathogenesis. The present study examined, first time best our knowledge, N-glycans as potential Kazakh population. profile 60...
Many genome- and epigenome-wide association studies (GWAS EWAS) of promoter methylation candidate genes for inflammatory bowel disease (IBD) have demonstrated significant associations between genetic epigenetic changes IBD. Independent GWA identified variants in the BACH2, IL6ST, LAMB1, IKZF1, MGAT3 loci to be associated with both IBD immunoglobulin G (IgG) glycosylation.Using bisulfite pyrosequencing, we analyzed CpG regions these five from peripheral blood several hundred patients healthy...
Glycosylation is one of the most common post-translation modifications with large influences on protein structure and function. The effector function immunoglobulin G (IgG) alters between pro- anti-inflammatory, based its glycosylation. IgG glycan synthesis highly complex dynamic.With use two different analytical methods for assessing glycosylation, we aim to elucidate link DNA methylation glycosylation by means epigenome-wide association studies. In total, 3000 individuals from 4 cohorts...
While N-linked glycosylation has been extensively studied in the context of inflammatory and metabolic disorders, its relationship with major depressive disorder (MDD) antidepressant treatment response not investigated. In our exploratory study, we analysed N-glycan profiles blood plasma samples collected from MDD patients (n = 18) found gender-dependent correlations severity symptoms prior to initiating treatment. addition, several N-glycosylation traits showed associations clinical...
Abstract It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having modulation of response. This first study investigating patients’ glycosylation profile diseases. Subclass specific was analyzed two cohorts allergen sensitized and non-sensitized 3- 11-year-old children (conducted at University Aberdeen, UK Children’s Hospital Srebrnjak, Zagreb, Croatia) with 893...