A5047560807- Gut microbiota and health
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- IL-33, ST2, and ILC Pathways
- Nutritional Studies and Diet
- Complementary and Alternative Medicine Studies
- Genetic Syndromes and Imprinting
- Digestive system and related health
- Effects of Radiation Exposure
- Probiotics and Fermented Foods
- Tuberculosis Research and Epidemiology
- Therapeutic Uses of Natural Elements
- Neutropenia and Cancer Infections
- Colorectal Cancer Screening and Detection
- Diverticular Disease and Complications
- Immune responses and vaccinations
- Inflammasome and immune disorders
- Medical and Biological Ozone Research
Genetic Analysis (Norway)
2015-2024
Summary Background Dysbiosis is associated with many diseases, including irritable bowel syndrome ( IBS ), inflammatory diseases IBD obesity and diabetes. Potential clinical impact of imbalance in the intestinal microbiota suggests need for new standardised diagnostic methods to facilitate microbiome profiling. Aim To develop validate a novel test using faecal samples profile identify characterise dysbiosis. Methods Fifty‐four DNA probes targeting ≥300 bacteria on different taxonomic levels...
Abstract Epigenetic alterations may provide important insights into gene-environment interaction in inflammatory bowel disease (IBD). Here we observe epigenome-wide DNA methylation differences 240 newly-diagnosed IBD cases and 190 controls. These include 439 differentially methylated positions (DMPs) 5 regions (DMRs), which study detail using whole genome bisulphite sequencing. We replicate the top DMP ( RPS6KA2 ) DMRs VMP1, ITGB2 TXK an independent cohort. Using paired genetic epigenetic...
Preclinical ulcerative colitis is poorly defined. We aimed to characterize the preclinical systemic inflammation in colitis, using a comprehensive set of proteins.We obtained plasma samples biobanked from individuals who developed later life (n = 72) and matched healthy controls 140) within population-based screening cohort. measured 92 proteins related proximity extension assay. The biologic relevance these findings was validated an inception cohort patients with 101) 50). To examine...
Background & AimsThe long-term efficacy and possible adverse events of fecal microbiota transplantation (FMT) for irritable bowel syndrome (IBS) are unknown. This study performed a 3-year follow-up the patients in our previous clinical trial to clarify these aspects.MethodsThis included 125 (104 females, 21 males): 38 placebo group, 42 who received 30 g donor feces, 45 60 feces. Feces was administered duodenum. The provided sample completed 5 questionnaires at baseline 2 3 years after FMT....
Imbalance in the microbiota, dysbiosis, has been identified inflammatory bowel disease (IBD). We explored fecal microbiota pediatric patients with treatment-naïve IBD, non-IBD gastrointestinal symptoms and healthy children, its relation to IBD subgroups, treatment outcomes.Fecal samples were collected from 235 children below 18 years of age. Eighty had Crohn's (CD), 27 ulcerative colitis (UC), 3 unclassified, 50 symptomatic patients, 75 healthy. The bacterial abundance 54 predefined DNA...
Abstract Aim To assess the pathobiological and translational importance of whole-blood transcriptomic analysis in inflammatory bowel disease [IBD]. Methods We analysed expression profiles from paired-end sequencing a discovery cohort 590 Europeans recruited across six countries IBD Character initiative (newly diagnosed patients with Crohn’s [CD; n = 156], ulcerative colitis [UC; 167], controls [n 267]), exploring differential [DESeq2], co-expression networks [WGCNA], transcription factor...
Abstract Background Epigenetic alterations may provide valuable insights into gene–environment interactions in the pathogenesis of inflammatory bowel disease [IBD]. Methods Genome-wide methylation was measured from peripheral blood using Illumina 450k platform a case-control study an inception cohort (295 controls, 154 Crohn’s [CD], 161 ulcerative colitis [UC], 28 IBD unclassified [IBD-U)] with covariates age, sex and cell counts, deconvoluted by Houseman method. Genotyping performed...
Abstract Background We recently found fecal microbiota transplantation (FMT) in irritable bowel syndrome (IBS) patients to be an effective and safe treatment after 3 months. The present follow‐up study investigated the efficacy safety of FMT at 1 year treatment. Methods This included 77 91 IBS who had responded our previous study. Patients provided a sample completed five questionnaires assess their symptoms quality life FMT. dysbiosis index (DI) bacterial profile were analyzed using 16S...
Background Faecal microbiota transplantation (FMT) seems to be a promising treatment for irritable bowel syndrome (IBS) patients. In Western countries (United States and Europe), there is female predominance in IBS. A sex difference the response FMT has been reported recently IBS Aim To investigate whether was patients who were included our previous randomized controlled trial of efficacy FMT. Methods The study 164 participated trial. These had moderate-to-severe symptoms belonging IBS-D...
Method We examined faecal samples, using the GA-map™ Dysbiosis Test, to associate gut microbiota composition with Crohn’s disease (CD) and ulcerative colitis (UC) identify markers for future biomarker identification. conducted a prospective case-control study (EU-ref. no. 305676) in an inception cohort of 324 individuals (64 CD, 84 UC, 116 symptomatic non-IBD controls 44 healthy controls) across five European centres 54 predetermined bacterial markers. categorized patients according Montreal...
Distinct faecal microbiota profiles are reported to be associated with various subtypes of IBS. Circulating antibodies cytolethal distending toxin B (CdtB) and vinculin proposed as biomarkers identify post-infectious The aim our study was analyse serum levels anti-CdtB anti-vinculin in patients different functional gastrointestinal disorders (FGID) their correlation the composition microbiome.The cohort comprised 65 prospectively recruited individuals: 15 diarrhoea-type-IBS (IBS-D), 13...
Compared to their healthy counterparts, patients with type 2 diabetes (T2D) can exhibit an altered gut microbiota composition, correlated detrimental outcomes, including reduced insulin sensitivity, dyslipidemia, and increased markers of inflammation. However, a typical T2D profile is not established. The aim this pilot study was explore the bacteria associated prediabetes (pre-T2D) patients, treatment naïve compared subjects.Fecal samples were collected from subjects (from Norway)....
Abstract Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota treatment-naïve ulcerative colitis (UC) patients determine microbial picture at start-up phase disease, using both a 16S rRNA transcript gene amplicon sequencing. DNA RNA were isolated from same colonic biopsies. Our aim was identify members in early stages disease reveal which are present, but do not act as...
Background and Aims: The mineral compound Luvos Healing Earth (LHE) is a commercially available remedy empirically used for variety of gastrointestinal disorders. aim this study was to investigate the possible effect prolonged LHE therapy on gut microbiota in healthy individuals patients with diarrhea-predominant irritable bowel syndrome (IBS-D). Methods: In prospective exploratory study, total 20 participants, including 12 controls 8 IBS-D, received treatment (Magenfein Granulat, 1 sachet...
P473 Histological inflammation in ulcerative colitis deep remission under treatment with infliximab A. Tursi1 *, W. Elisei2, M. Picchio3, G. Forti4, Penna5, P.G. Lecca6, Di Fonzo6, L. Cesare6, Brandimarte6. 1Azienda Sanitaria Locale BAT, Servizio di Gastroenterologia Territoriale, Andria, Italy, 2ASL RMH, Division of Gastroenterology, Albano Laziale, 3“P. Colombo” Hospital, ASL Surgery, Velletri, 4“S. Maria Goretti” Digestive Endoscopy Unit, Latina, 5“S. Paolo” Bari, 6“Cristo Re” Rome, Italy
Science S81Conclusions: These data show a strong correlation of inflammatory responses between the colitis and radiationinduced models GI injury, with common TNF-mediated, neutrophilic component in colon jejunum, respectively.Further studies will investigate time course tissue differences.The efficacy AVX-470m both highlights central role TNF tract inflammation, supports therapeutic potential an oral anti-TNF antibody disease.
Poster presentations to a labeled end of an amplified gene.T-RFLP is established technique for analysing bacterial species in faecal samples.Methods: This was quantitative analysis with major focus on evaluating biomarker based intestinal microbiota.The subjects were 69 UC patients together 80 relatives as controls.Twenty-three had active (group I) and 46 quiescent II).The latter included 17 mild inflammation the large intestine IIa), 29 without IIb).The patients' consanguineous III, n =...
Background: Knowledge about a patients' microbiota profiles might give useful information in diagnosing, early relapse prediction and to distinguish responders from non-responders treatment. Fecal calprotectin (FCal) is used as marker diagnosis follow-up of patients with inflammatory bowel diseases (IBD). The IBD-Character project aims analyse fecal profiles, microbial diversity concentration FCal treatment naive newly diagnosed IBD patients, symptomatic non-IBD healthy controls. Methods:...
Background: Imbalance in the faecal microbiota with a reduction biodiversity; dysbiosis, has been identified inflammatory bowel disease (IBD). Our aim was to study and compare paediatric patients newly diagnosed untreated IBD of healthy children gastrointestinal symptoms but no IBD. We also aimed at studying related subgroups treatment. Methods: Faecal samples were collected from 235 adolescents. Eighty had Crohn's (CD), 27 ulcerative colitis (UC) 3 unclassified, 50 non-IBD 75 between two 18...
Ulcerative colitis (UC) is a chronic inflammatory disease affecting the large intestine. The course varies from an indolent to aggressive disease, requiring early introduction of biologics and colectomy in treatment refractory individuals. There clinical need biomarkers that can be used predict future already at diagnosis. Microbiota signatures might help this respect could potentially become tool for implementation personalised medicine. Fecal samples were collected diagnosis 47...