A5085177083- Inflammatory Bowel Disease
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- IL-33, ST2, and ILC Pathways
- Digestive system and related health
- Eosinophilic Esophagitis
- Immunodeficiency and Autoimmune Disorders
- Drug Transport and Resistance Mechanisms
- Liver Diseases and Immunity
- Inflammasome and immune disorders
- Immune cells in cancer
- Tuberculosis Research and Epidemiology
- Diagnosis and treatment of tuberculosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mycobacterium research and diagnosis
- Epigenetics and DNA Methylation
- Gastrointestinal motility and disorders
- Celiac Disease Research and Management
- Immune Cell Function and Interaction
- Pediatric Hepatobiliary Diseases and Treatments
- Metabolism and Genetic Disorders
- Intensive Care Unit Cognitive Disorders
University of Edinburgh
2016-2025
Centre for Inflammation Research
2016-2025
Institute of Infection and Immunity
2025
University of Glasgow
2025
MRC Centre for Regenerative Medicine
2025
Centre de Recherche sur l'Inflammation
2025
Western General Hospital
2009-2024
Institute of Genetics and Cancer
2013-2023
Queen's Medical Centre
2020-2023
University of Dundee
2023
Abstract Epigenetic alterations may provide important insights into gene-environment interaction in inflammatory bowel disease (IBD). Here we observe epigenome-wide DNA methylation differences 240 newly-diagnosed IBD cases and 190 controls. These include 439 differentially methylated positions (DMPs) 5 regions (DMRs), which study detail using whole genome bisulphite sequencing. We replicate the top DMP ( RPS6KA2 ) DMRs VMP1, ITGB2 TXK an independent cohort. Using paired genetic epigenetic...
<ns4:p>Inflammatory bowel diseases are common, complex, immune-mediated conditions with a sharply rising global prevalence. While major advances since 2000 have provided strong mechanistic clues implicating de-regulation in the normal interaction among host genetics, immunity, microbiome, and environment, more recent progress has generated entirely new hypotheses also further refined older disease concepts. In this review, we focus specifically on these novel developments pathogenesis of...
Objective IBD prevalence is estimated to be rising, but no detailed, recent UK data are available. The last reported estimate in the was 0.40% 2003. We aimed establish current, and project future, Lothian, Scotland. Design conducted an all-age multiparameter search strategy using inpatient international classification of disease (ICD-10) coding (K50/51)(1997–2018), pathology (1990–2018), primary secondary care prescribing (2009–2018) a paediatric registry, (1997–2018) identify ‘possible’...
INTRODUCTION: The joint associations across genetic risk, modifiable lifestyle factors, and inflammatory bowel disease (IBD) remains unclear. METHODS: Genetic susceptibility to Crohn's (CD) ulcerative colitis (UC) was estimated by polygenic risk scores further categorized into high, intermediate, low categories. Weighted healthy were constructed based on 5 common factors favorable (4 or factors), intermediate (3 unfavorable (0–2 factors) groups. Cox proportional hazards regression model used...
The failure rate of medical therapy in severe ulcerative colitis is high. A risk index, to aid the identification patients not responding at an early stage intravenous corticosteroid therapy, would be useful facilitate second-line treatment or surgery.We recruited 167 consecutive with between January 1995 and March 2002; employed multiple logistic regression analyse parameters within first 3 days therapy. We applied statistical modelling formulate a score according likelihood...
Summary Background Corticosteroids remain the mainstay of first‐line therapy in active inflammatory bowel disease. Aims To determine clinical outcome after first corticosteroid‐therapy and to identify factors which predict response/failure. Methods 216 (136 ulcerative colitis 80 Crohn's disease) patients were identified this 5‐year inception cohort. The outcomes early (30 days) late (1 year) responses used. Multivariate analyses performed associated with outcome. Results 86 (63%) 60 (75%)...
<h3>Objective:</h3> To investigate differential intestinal gene expression in patients with ulcerative colitis and controls. <h3>Design:</h3> Genome-wide study (41 058 sequence tags, 215 biopsies). <h3>Setting:</h3> Western General Hospital, Edinburgh, UK, Genentech, San Francisco, USA. <h3>Patients:</h3> 67 31 control subjects (23 normal 8 inflamed non-inflammatory bowel disease <h3>Interventions:</h3> Paired endoscopic biopsies were taken from 5 specific anatomical locations for RNA...
OBJECTIVES: Calprotectin is a granulocyte neutrophil-predominant cytosolic protein. Fecal concentrations are elevated in intestinal inflammation and may predict relapse quiescent inflammatory bowel disease. We aim to investigate fecal calprotectin (FC) as biomarker predicting the clinical course of acute severe ulcerative colitis (ASUC). METHODS: In 90 patients with ASUC requiring intensive in-patient medical therapy (January 2005-September 2007), we investigated discriminant ability FC...
<ns4:p>Mitochondrial DNA (mtDNA) has many similarities with bacterial because of their shared common ancestry. Increasing evidence demonstrates mtDNA to be a potent danger signal that is recognised by the innate immune system and can directly modulate inflammatory response. In humans, elevated circulating found in conditions significant tissue injury such as trauma sepsis increasingly chronic organ-specific systemic illnesses steatohepatitis lupus erythematosus. this review, we examine our...
There is an unmet need for novel blood-based biomarkers that offer timely and accurate diagnostic prognostic testing in inflammatory bowel diseases (IBD). We aimed to investigate the utility of serum calprotectin (SC) IBD.A total 171 patients (n=96 IBD, n=75 non-IBD) were prospectively recruited. A multi-biomarker model was derived using multivariable logistic regression analysis. Cox proportional hazards assess contribution each variable disease outcomes.SC correlated strongly with current...
Abstract Background Due to common evolutionary origins, mitochondrial DNA (mtDNA) shares many similarities with immunogenic bacterial DNA. MtDNA is recognized as a pro-inflammatory damage-associated molecular pattern (DAMP) pathogenic role in several inflammatory diseases. We hypothesised that mtDNA released during active disease, serving key factor bowel disease (IBD). Methods Between 2014 and 2015, we collected plasma separated within 2 hours of sampling from 97 prospectively recruited IBD...
Preclinical ulcerative colitis is poorly defined. We aimed to characterize the preclinical systemic inflammation in colitis, using a comprehensive set of proteins.We obtained plasma samples biobanked from individuals who developed later life (n = 72) and matched healthy controls 140) within population-based screening cohort. measured 92 proteins related proximity extension assay. The biologic relevance these findings was validated an inception cohort patients with 101) 50). To examine...
PGE 2 inhibits T regs and promotes intestinal inflammation through actions on mononuclear phagocytes the gut microbiota.
Abstract This work aims to investigate how smoking exerts effect on the development of inflammatory bowel disease (IBD). A prospective cohort study and a Mendelian randomization are first conducted evaluate association between behaviors, smoking-related DNA methylation risks Crohn’s (CD) ulcerative colitis (UC). We then perform both genome-wide analysis co-localization validate observed associations. Compared never smoking, current previous habits associated with increased CD ( P = 7.09 × 10...
Summary Background Forty per cent of patients with acute severe ulcerative colitis will not respond to intravenous corticosteroids and require second‐line medical therapy or colectomy. A recent controlled trial has suggested that infliximab may be effective as rescue therapy. Aim To assess the value for in a retrospective cohort Scotland. Methods All satisfied Truelove Witts criteria on admission, failed received (5 mg/kg) Response was defined need colectomy at hospital discharge by 90 days....