Florent Clerc
A5016336771- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Epigenetics and DNA Methylation
- Polymer Surface Interaction Studies
- IL-33, ST2, and ILC Pathways
- Dendrimers and Hyperbranched Polymers
- Digestive system and related health
- Genetic Syndromes and Imprinting
- Inflammatory Bowel Disease
- Mass Spectrometry Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Luminescence and Fluorescent Materials
- Renal Diseases and Glomerulopathies
- Celiac Disease Research and Management
- Chronic Lymphocytic Leukemia Research
Leiden University Medical Center
2015-2023
Netherlands Metabolomics Centre
2015-2021
University of Geneva
2012
Abstract Epigenetic alterations may provide important insights into gene-environment interaction in inflammatory bowel disease (IBD). Here we observe epigenome-wide DNA methylation differences 240 newly-diagnosed IBD cases and 190 controls. These include 439 differentially methylated positions (DMPs) 5 regions (DMRs), which study detail using whole genome bisulphite sequencing. We replicate the top DMP ( RPS6KA2 ) DMRs VMP1, ITGB2 TXK an independent cohort. Using paired genetic epigenetic...
IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and a leading cause of renal failure. The mechanisms are not completely understood, but higher abundance galactose-deficient recognized to play crucial role in IgAN pathogenesis. Although both types human (IgA1 IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation. galactose deficiency on O-glycans commonly detected by lectin-based method. To...
Glycosylation is a post-translational modification of key importance with heterogeneous structural characteristics. Previously, we have developed robust, high-throughput MALDI-TOF-MS method for the comprehensive profiling human plasma N-glycans. In this approach, sialic acid residues are derivatized linkage-specificity, namely ethylation α2,6-linked parallel lactone formation α2,3-linked acids. current study, procedure was used as starting point automation all steps on liquid-handling robot...
Background & AimsDNA methylation alterations may provide important insights into gene-environment interaction in cancer, aging, and complex diseases, such as inflammatory bowel disease (IBD). We aim first to determine whether the circulating DNA methylome patients requiring surgery predict Crohn's (CD) recurrence following intestinal resection; second compare seen with established CD that we had reported a series of inception cohorts.MethodsTOPPIC was placebo-controlled, randomized...
Inflammatory bowel diseases (IBD), such as Crohn's disease (CD) and ulcerative colitis (UC), are chronic relapsing inflammations of the digestive tract with increasing prevalence, yet they have unknown origins or cure. CD UC similar symptoms but respond differently to surgery medication. Current diagnostic tools often involve invasive procedures, while laboratory markers for patient stratification lacking. Large glycomic studies immunoglobulin G total plasma glycosylation shown biomarker...
By combining quartz crystal microbalance (QCM) and reflectivity, it was shown that poly(amidoamine) (PAMAM) dendrimers adsorbed at the silica–water interface undergo conformational changes when incubated in various electrolyte solutions. Adsorbed swell solutions of high ionic strengths low pH, while they shrink pH. Dendrimer swelling is further ion specific, whereby cations influence to a larger extent than anions. Among studied, magnesium ions induce largest swelling, lithium smallest. The...