Frano Vučković
A5005810527- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- Immunodeficiency and Autoimmune Disorders
- Clostridium difficile and Clostridium perfringens research
- Blood groups and transfusion
- Advanced Proteomics Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Diabetes Treatment and Management
- Dietary Effects on Health
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Metabolomics and Mass Spectrometry Studies
- Glycogen Storage Diseases and Myoclonus
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
Science Research Laboratory
2015-2024
Genos (Croatia)
2015-2024
University of Zagreb
2013
Fine structural details of glycans attached to the conserved N-glycosylation site significantly not only affect function individual immunoglobulin G (IgG) molecules but also mediate inflammation at systemic level. By analyzing IgG glycosylation in 5,117 individuals from four European populations, we have revealed very complex patterns changes with age. Several (including FA2B, FA2G2, and FA2BG2) changed considerably age combination these three can explain up 58% variance chronological age,...
Objective Glycans attached to the Fc portion of IgG are important modulators effector functions. Interindividual differences in glycome composition large and they associate strongly with different inflammatory autoimmune diseases. IKZF1 , HLA–DQ2A/B BACH2 genetic loci that affect show pleiotropy systemic lupus erythematosus (SLE), indicating a potentially causative role aberrant glycosylation SLE. We undertook this multicenter case–control study determine whether SLE is associated altered...
Abstract Epigenetic alterations may provide important insights into gene-environment interaction in inflammatory bowel disease (IBD). Here we observe epigenome-wide DNA methylation differences 240 newly-diagnosed IBD cases and 190 controls. These include 439 differentially methylated positions (DMPs) 5 regions (DMRs), which study detail using whole genome bisulphite sequencing. We replicate the top DMP ( RPS6KA2 ) DMRs VMP1, ITGB2 TXK an independent cohort. Using paired genetic epigenetic...
Glycobiology is an underexplored research area in inflammatory bowel disease (IBD), and glycans are relevant to many etiological mechanisms described IBD. Alterations N-glycans attached the immunoglobulin G (IgG) Fc fragment can affect molecular structure immunological function. Recent genome-wide association studies reveal pleiotropy between IBD IgG glycosylation. This study aims explore glycan changes ulcerative colitis (UC) Crohn's (CD).IgG glycome composition patients with UC (n = 507),...
Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation. Protein glycosylation essential many steps inflammatory cascade, but due technological limitations role variation inflammation not been addressed until now. We analysed composition total plasma IgG N-glycomes 107 patients undergoing surgery. In nearly all individuals N-glycome underwent same pattern changes first 72 h,...
Alternative glycosylation has significant structural and functional consequences on IgG consequently also cancer immunosurveillance. Because of technological limitations, the effects highly heritable individual variations differences in dynamics changes colorectal were never investigated before.
Effector functions of immunoglobulin G (IgG) are regulated by the composition a glycan moiety, thus affecting activity immune system. Aberrant glycosylation IgG has been observed in many diseases, but little is understood about underlying mechanisms. We performed genome-wide association study N-glycosylation (
Poorer glycemic control in type 1 diabetes may alter N-glycosylation patterns on circulating glycoproteins, and these alterations be linked with diabetic kidney disease (DKD). We investigated associations between N-glycans renal function diabetes.Using serum samples from 818 adults who were considered to have extreme annual loss estimated glomerular filtration rate (eGFR; i.e., slope) based retrospective clinical records, among 6,127 the Scottish Diabetes Research Network Type Bioresource...
Abstract In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker colorectal cancer (CRC). We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes. assessed predictive clinical algorithms compared to that also included glycan predictors. Decreased galactosylation, decreased sialylation (of fucosylated structures) increased bisecting GlcNAc structures were strongly associated all-cause (q <...
Plasma protein N-glycan profiling integrates information on enzymatic glycosylation, which is a highly controlled ubiquitous posttranslational modification. Here we investigate the ability of plasma N-glycome to predict incidence type 2 diabetes and cardiovascular diseases (CVDs; i.e., myocardial infarction stroke).Based prospective European Prospective Investigation Cancer (EPIC)-Potsdam cohort (n = 27,548), constructed case-cohorts including random subsample 2,500 participants all...
Glycans expand the structural complexity of proteins by several orders magnitude, resulting in a tremendous analytical challenge when including them biomedical research. Recent glycobiological research is painting picture which glycans represent crucial and functional component majority proteins, with alternative glycosylation lipids being an important regulatory mechanism many biological pathological processes. Since interindividual differences are extensive, large studies needed to map...
ObjectiveTo determine the extent to which genetic and epigenetic factors contribute variations in glycosylation of immunoglobulin G (IgG) humans. Methods76 N-glycan traits circulating IgG were analyzed by UPLC 220 monozygotic 310 dizygotic twin pairs from TwinsUK. A classical study design was used derive additive genetic, common unique environmental components defining variance these traits. Epigenome-wide association analysis performed using Illumina 27k chip. Results51 76 glycan studied...
Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays major role in the human adaptive immune response and are associated to wide range of diseases. Glycosylation Fc binding region IgGs, responsible for antibody's effector function, is essential prompting proper response. This study focuses on general genetic impact IgG glycosylation as well corresponding subclass-specificities. To identify loci involved glycosylation, we performed genome-wide association (GWAS) liquid...
Aberrant glycosylation has been associated with a number of diseases including cancer. Our aim was to elucidate changes in whole plasma N-glycosylation between colorectal cancer (CRC) cases and controls one the largest cohorts its kind. A set 633 CRC patients 478 age gender matched analysed. Additionally, were stratified into four stages. Moreover, N-glycan analysis carried out 40 collected prior initial diagnosis CRC. Statistically significant differences observed N-glycome at all stages...
Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through attachment of various sugar moieties called glycans. Composition IgG N-glycome changes with age an individual in different diseases. Variability glycosylation within a population well studied known to be affected by both genetic environmental factors. However, global inter-population differences have never been properly addressed. Here we present...
Background: Most respiratory viruses show pronounced seasonality, but for SARS-CoV-2, this still needs to be documented. Methods: We examined the disease progression of COVID-19 in 6,914 patients admitted hospitals Europe and China. In addition, we evaluated progress symptoms 37,187 individuals reporting into COVID Symptom Study application. Findings: Meta-analysis mortality risk seven European estimated odds ratios per 1-day increase admission date 0.981 (0.973–0.988, p &lt; 0.001)...
Obesity, a major global health problem, is associated with increased cardiometabolic morbidity and mortality. Protein glycosylation frequent posttranslational modification, highly responsive to inflammation ageing. The prospect of biological age reduction, by changing patterns through metabolic intervention, opens many possibilities. We have investigated whether weight loss interventions affect inflammation- ageing-associated IgG changes, in longitudinal cohort bariatric surgery patients. To...
Abstract Post-translational modifications diversify protein functions and dynamically coordinate their signalling networks, influencing most aspects of cell physiology. Nevertheless, genetic regulation or influence on complex traits is not fully understood. Here, we compare the same PTM two proteins – glycosylation transferrin immunoglobulin G (IgG). By performing genome-wide association analysis glycosylation, identify 10 significantly associated loci, 9 which were reported previously....
Coronary artery disease (CAD) is the most common cardiovascular (CVD), and previous studies have shown a significant association between N-glycosylation, highly regulated posttranslational modification, development of atherosclerotic plaques. Our aim was to determine whether N-glycome immunoglobulin G (IgG) associated with CAD, as N-glycans are known alter effector functions IgG, which may enhance inflammatory response in CAD. Therefore, this study, we isolated IgG from subjects coronary...
Young blood plasma is known to confer beneficial effects on various organs in mice and rats. However, it was not whether from young adult pigs rejuvenates old rat tissues at the epigenetic level; alters clock, which a highly accurate molecular biomarker of aging. To address this question, we developed validated six different clocks for that are based DNA methylation values derived n = 613 tissue samples. As indicated by their respective names, pan-tissue clock can be applied profiles all...