A5029677872- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Down syndrome and intellectual disability research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Chronic Lymphocytic Leukemia Research
- Sodium Intake and Health
- Immune Cell Function and Interaction
- Microfluidic and Capillary Electrophoresis Applications
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Muscle metabolism and nutrition
- Electrochemical sensors and biosensors
- Genetics and Neurodevelopmental Disorders
- Electrochemical Analysis and Applications
- Metabolism, Diabetes, and Cancer
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
Science Research Laboratory
2020-2025
Genos (Croatia)
2020-2025
University of Zagreb
2017
Obesity, a major global health problem, is associated with increased cardiometabolic morbidity and mortality. Protein glycosylation frequent posttranslational modification, highly responsive to inflammation ageing. The prospect of biological age reduction, by changing patterns through metabolic intervention, opens many possibilities. We have investigated whether weight loss interventions affect inflammation- ageing-associated IgG changes, in longitudinal cohort bariatric surgery patients. To...
Gonadal hormones affect immunoglobulin G (IgG) glycosylation, and the more proinflammatory IgG glycome composition might be one of molecular mechanisms behind increased phenotype in perimenopause. Using ultra-high-performance liquid chromatography, we analyzed 5,080 samples from 1940 pre-, peri-, postmenopausal women. Statistically significant decrease galactosylation sialylation was observed Furthermore, during transition pre- to period, rate increase agalactosylated structures (0.051/yr;...
People with Down syndrome (DS) show clinical signs of accelerated ageing. Causative mechanisms remain unknown and hypotheses range from the (essentially untreatable) amplified-chromosomal-instability explanation, to potential actions individual supernumerary chromosome-21 genes. The latter explanation could open a route therapeutic amelioration if specific over-acting genes be identified their action toned-down.
Nuclear magnetic resonance (NMR) spectra of blood serum and plasma show signals arising from metabolites, lipoproteins, N-acetyl methyl groups N-glycans covalently linked to acute-phase proteins. These glycan often called glycoprotein A (GlycA) B (GlycB) arise have been proposed as biomarkers, initially for cardiovascular diseases, but also other inflammatory conditions. For the detection resonances, J-edited, diffusion, relaxation filtered NMR spectroscopy (JEDI) has suppress lipoprotein...
Crohn’s disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients’ quality life and well-being. Anti-TNF biologicals revolutionised treatment CD, yet many patients do not adequately respond to such therapy. Previous studies have demonstrated pro-inflammatory pattern in composition CD immunoglobulin G (IgG) N-glycome compared healthy individuals. Here, we utilised high-throughput UHPLC method for N-glycan analysis explore longitudinal effect anti-TNF...
Objectives: Glycosylation of immunoglobulin G (IgG) is an important regulator the immune system and has been implicated in prevalent hypertension. The aim this study to investigate whether IgG glycome begins change prior hypertension diagnosis by analysing composition a large population-based female cohort with two independent replication samples. Methods: We included 989 unrelated cases incident 1628 controls from TwinsUK (mean follow-up time 6.3 years) measured at baseline...
Obesity-induced inflammation activates the adaptive immune system by altering immunoglobulin G (IgG) glycosylation in a way to produce more proinflammatory antibodies. The IgG glycome has already been well studied, and its alterations are correlated with high body mass index (BMI) central adiposity. Still, N-glycome susceptibility different dietary regimes for weight control after initial loss not studied. To explore changes induced subsequent weight-maintenance diets, we analyzed 1,850...
Abstract Background Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of melanoma and other cancers. However, no reliable biomarker survival or response has entered clinic to identify those patients with who are most likely benefit from ICIs. Glycosylation affects proteins lipids’ structure functions. Tumours characterized by aberrant glycosylation which may contribute their progression hinder an effective antitumour immune response. Methods We aim at identifying novel...
Regular exercise improves health, modulating the immune system and impacting inflammatory status. Immunoglobulin G (IgG) N-glycosylation reflects changes in status; thus, we investigated impact of regular on overall status by monitoring IgG a previously inactive, middle-aged, overweight obese population (50.30 ± 9.23 years, BMI 30.57 4.81). Study participants (N = 397) underwent one three different programs lasting months with blood samples collected at baseline end intervention. After...
Abstract Protein glycosylation is the attachment of a carbohydrate moiety to protein backbone affecting both structure and function protein. Abnormal associated with various diseases, some changes in are detectable even before symptom development. As such, glycans have emerged as compelling new biomarker candidates. A wide range analytical methods exist for small-scale glycan analyses. However, there growing need highly robust reproducible high-throughput techniques that allow large-scale...
ABSTRACT Background Obesity is a major global health problem, and associated with increased cardiometabolic morbidity mortality. Protein glycosylation frequent postranslational modification, highly responsive to numerous pathophysiological conditions ageing. The prospect of biological age reduction, by reverting changes through metabolic intervention, opens many possibilities. We have investigated whether weight loss interventions affect inflammation- ageing-associated IgG changes, in...
Abstract Cells from people with Down syndrome (DS) show faster accumulation of DNA damage and epigenetic aging marks. Causative mechanisms remain un-proven hypotheses range amplified chromosomal instability to actions several supernumerary chromosome 21 genes. Plasma immunoglobulin G (IgG) glycosylation profiles are established as a reliable predictor biological chronological aging. We performed IgG glycan profiling n=246 individuals DS (208 adults 38 children) three European populations...
Aberrant plasma protein glycosylation is associated with a wide range of diseases, including diabetes, cardiovascular, and immunological disorders. To investigate alterations due to weight loss successive weight-maintenance diets, 1850 glycomes from participants the Diogenes study were analyzed using Ultra-High-Performance Liquid Chromatography (UHPLC). The large dietary intervention in which subjected low-calorie diet (LCD) followed by one five different diets period 6 months. most notable...
In a subset of participants from the CALERIE™ Phase 2 study we evaluated effects 2y ∼25% Calorie Restriction (CR) diet on IgG N-glycosylation (GlycAge), plasma and complement C3 N-glycome as markers aging inflammaging.
Background: Glycosylation of immunoglobulin G (IgG) is an important regulator the immune system and its changes are believed to be a significant contributor inflammaging. Gonadal hormones affect IgG glycome composition, suggesting that alterations in glycosylation might one molecular mechanisms behind increased disease risk perimenopause.Methods: was isolated from 5,354 plasma samples collected 1,940 females 113 males at multiple time points. glycans were released, labelled with fluorescent...
Objective: N-glycosylation, one of the most complex co- and post-translational modifications, is a highly regulated process influenced by many diseases. There evidence that N-glycosylation could be marker hypertension. The aim this study was to determine if plasma N-glycome predictor incident hypertension (HTN). Design method: We selected 271 unrelated cases with controls matched for age, BMI follow-up time from TwinsUK cohort (mean 8.5 years). Longitudinal (39 directly measured traits)...
Abstract Background Glycosylation of immunoglobulin G (IgG) is an important regulator the immune system and its changes are believed to be a significant contributor inflammaging. Gonadal hormones affect IgG glycome composition, suggesting that alterations in glycosylation might one molecular mechanisms behind increased disease risk perimenopause. Methods was isolated from 5,354 plasma samples collected 1,940 females 113 males at multiple time points. glycans were released, labelled with...