A5060597852- Hippo pathway signaling and YAP/TAZ
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Protein purification and stability
- Analytical Chemistry and Chromatography
- Extracellular vesicles in disease
- RNA modifications and cancer
- Advanced Proteomics Techniques and Applications
- Synthetic Organic Chemistry Methods
- Petroleum Processing and Analysis
- Hydrocarbon exploration and reservoir analysis
- Analytical Methods in Pharmaceuticals
- Medical Imaging and Pathology Studies
- Microbial Natural Products and Biosynthesis
- Cell death mechanisms and regulation
- Platelet Disorders and Treatments
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- Pharmacogenetics and Drug Metabolism
- Listeria monocytogenes in Food Safety
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Marine Sponges and Natural Products
- Neonatal Respiratory Health Research
Health Canada
2012-2021
Structural Genomics Consortium
2010
University of Manitoba
2010
Queen's University
2010
University of Toronto
2010
Center for Biologics Evaluation and Research
2001
Centre for Drug Research and Development
1994
University of Alberta
1984-1986
University of Victoria
1980-1982
The large tumor suppressor 1 (LATS1) is a serine/threonine kinase and found down-regulated in broad spectrum of human cancers. LATS1 central player the emerging Hippo-LATS pathway, which plays important roles cell proliferation, apoptosis, stem differentiation. Despite ample data supporting role for suppression, how regulated at molecular level remains largely unknown. In this study, we have identified Itch, HECT class E3 ubiquitin ligase, as unique binding partner LATS1. Itch can complex...
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Abstract Platelet αIIbβ3 integrin and its ligands are essential for thrombosis hemostasis, play key roles in myocardial infarction stroke. Here we show that apolipoprotein A-IV (apoA-IV) can be isolated from human blood plasma using platelet β3 integrin-coated beads. Binding of apoA-IV to platelets requires activation integrin, the direct apoA-IV-αIIbβ3 interaction detected a single-molecule Biomembrane Force Probe. We identify aspartic acids 5 13 at N-terminus required binding which is...
To determine whether the clinical adverse interactions of terfenadine with azole antifungals and macrolide antibiotics may be related to inhibition biotransformation, an in vitro system was developed follow metabolism by rat liver S9 or human microsomes. When test compounds were coincubated terfenadine, metabolites formed unchanged quantitatively analyzed HPLC. Five S9: predominantly alcohol metabolite (III), four minor metabolites--azacyclonol (I), acid (II), unidentified (IV), a new ketone...
Abstract Although antitubulin drugs are used widely to treat human cancer, many patients display intrinsic or acquired drug resistance that imposes major obstacles successful therapy. Mounting evidence argues cancer cell apoptosis triggered by relies upon activation of the cell-cycle kinase Cdk1; however, mechanistic connections this event remain obscure. In study, we identified antiapoptotic protein YAP, a core component Hippo signaling pathway implicated in tumorigenesis, as critical...
Inactivation of intact influenza viruses using formaldehyde or β-propiolactone (BPL) is essential for vaccine production and safety. The extent chemical modifications such reagents on viral proteins needs to be extensively investigated better control the reactions quality vaccines. We have evaluated effect BPL inactivation two candidate re-assortant vaccines (NIBRG-121xp NYMC-X181A) derived from A/California/07/2009 pandemic high-resolution FT-ICR MS-based proteomic approaches. report here...
The gene (bviA) encoding the ruminal bacteriocin butyrivibriocin AR10 was cloned from an EcoRI library by using oligonucleotide probe based on a partial peptide sequence of previously isolated peptide. encoded 80 amino acid prebacteriocin that demonstrated significant identity with cyclic gassericin A. Negative ion time flight mass spectroscopic analysis (ESI/MS) indicated 5981.5 Da for bacteriocin, molecular could not be generated removal leader alone. However, N- to C-terminal cyclization...
Biofilm formation may be important in the colonization of food-processing environment by food-borne pathogen Listeria monocytogenes. monocytogenes 568 formed adherent multicellular layers on a variety test surfaces following growth at 37 degrees C with multiple transfers surface into fresh medium. Microscopic examination these suggest that cells were surrounded extracellular material. The presence carbohydrate containing polymeric matrix was confirmed labelling hydrated...
In this work, we report on the applicability of two-dimensional high-performance liquid chromatography (2D-HPLC) for comprehensive characterization inactivated influenza vaccine proteins. This novel procedure features minimal sample treatment and combines on-line coupling size exclusion HPLC to reversed-phase HPLC. A comparative analysis commercial vaccines from three different manufacturers showed method be highly selective by providing characteristic reproducible chromatographic profiles...
The outbreak of a pandemic influenza H1N1 in 2009 required the rapid generation high-yielding vaccines against A/California/7/2009 virus, which were achieved by either addition or deletion glycosylation site proteins hemagglutinin and neuraminidase. In this report, we have systematically evaluated glycan composition, structural distribution topology for two high-yield candidate reassortant (NIBRG-121xp NYMC-X181A) combining various enzymatic digestions with high performance liquid...
Cellular immunity is important for protection against the serious complications of influenza in older adults. As it unclear if newer vaccines elicit greater cellular responses than standard vaccines, we compared to 2 and licensed trivalent inactivated (TIVs) a randomized trial Non-frail adults ≥ 65 y old were randomly assigned receive subunit, MF59-adjuvanted split-virus or intradermal TIV. Peripheral blood mononuclear cells (PBMC) harvested pre- 3-weeks post-vaccination stimulated with live...
ABSTRACT Listeria innocua 743 produces an inhibitory activity demonstrating broad-spectrum inhibition of monocytogenes isolates. Gel-electrophoretic analysis culture supernatants indicated that two inhibitors with different molecular weights were produced by this strain. Insertion Tn 917 into a 2.9 Kb plasmid (pHC743) generated mutants either impaired ability or loss in to produce one the inhibitors. Sequence transposon insertion regions revealed presence continuous open reading frames,...
The fusion peptide of influenza virus hemagglutinin (HA) has a critical role in mediating the entry into cells and is also only universally conserved sequence HAs all strains A B viruses. Therefore, it could be an attractive target for new vaccine development potency marker existing vaccines. epitope hidden inside HA proteins, making inaccessible quantitative antibody binding. Our simple slot blot protocol highlights pre-treatment samples with moderate concentrations denaturant to maximally...