A5067804809- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Protein purification and stability
- Reproductive Physiology in Livestock
- Proteoglycans and glycosaminoglycans research
- Metabolism and Genetic Disorders
- Genetic and phenotypic traits in livestock
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Diabetes and associated disorders
- Erythrocyte Function and Pathophysiology
- Transgenic Plants and Applications
- Milk Quality and Mastitis in Dairy Cows
- Reproductive tract infections research
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Glycogen Storage Diseases and Myoclonus
- Viral Infectious Diseases and Gene Expression in Insects
- Alcohol Consumption and Health Effects
- Digestive system and related health
- Innovative Microfluidic and Catalytic Techniques Innovation
- Amino Acid Enzymes and Metabolism
National Institute for Bioprocessing Research and Training
2015-2024
National University of Ireland, Maynooth
2012-2024
The University of Melbourne
2013
Glycosylation is a topic of intense current interest in the development biopharmaceuticals because it related to drug safety and efficacy. This work describes results an interlaboratory study on glycosylation Primary Sample (PS) NISTmAb, monoclonal antibody reference material. Seventy-six laboratories from industry, university, research, government, hospital sectors Europe, North America, Asia, Australia submitted total 103 reports glycan distributions. The principal objective this was...
Abstract A significant proportion of the infant gut microbiome is considered to be acquired from mother during and after birth. Thus begins a lifelong dynamic relationship with microbes that has an enduring impact on host health. Based cohort 135 mother-infant (F = 72, M 63) dyads (MicrobeMom: ISRCTN53023014), we investigated phenomenon microbial strain transfer, particular emphasis use combined metagenomic-culture-based approach determine frequency transfer involving members genus...
N-Glycosylation is a fundamentally important protein modification with major impact on glycoprotein characteristics such as serum half-life and receptor interaction. More than half of the proteins in human are glycosylated, relative abundances glycoforms often reflect alterations health disease. Several analytical methods currently capable analyzing total N-glycosylation high-throughput manner.Here we evaluate compare performance three released N-glycome analysis methods. Included were...
Complex carbohydrates are rapidly becoming excellent biomarker candidates because of their high sensitivity to pathological changes. However, the discovery clinical glycobiomarkers has been slow, due scarcity high-throughput glycoanalytical workflows that allow rapid glycoprofiling large sample sets. To generate high-quality quantitative glycomics data in a fashion, we have developed robotized platform for serum-based N-glycan preparation. The preparation workflow features fully automated,...
Abstract Early diagnosis and dietary treatment do not prevent long-term complications, which mostly affect the central nervous system in classical galactosemia patients. The clinical outcome of patients is highly variable, there an urgent need for prognostic biomarkers. aim this study was first to increase knowledge on natural history by studying a cohort with varying geno- phenotypes second association between outcomes two possible In addition, abnormalities brain MRI investigated....
Background: Glycosylation is one of the most fundamental post-translational modifications. Importantly, glycosylation altered in many cancers. These alterations have been proven to impact on tumour progression and promote cell survival. From literature, it known that there a clear link between chemoresistance hypoxia, hypoxia epigenetics more recently epigenetics. Methods Results: Our objective was investigate these differential parameters, an vitro model ovarian breast cancer. Ovarian...
N-glycan alterations in the nervous system can result different neuropathological symptoms such as mental retardation, seizures, and epilepsy. Studies have reported characterization of N-glycans rodent brains, but there is a lack spatial resolution either tissue samples were homogenized or specific proteins selected for analysis glycosylation. We hypothesize that region-specific isolated from striatum substantia nigra (SN) give an insight into establishment pathophysiological degeneration...
Glucuronosyl diacylglycerides (GlcAGroAc2) are functionally important glycolipids and membrane anchors for cell wall lipoglycans in the Corynebacteria. Here we describe complete synthesis of distinct acyl-isoforms GlcAGroAc2 bearing both acylation patterns (R)-tuberculostearic acid (C19:0) palmitic (C16:0) their mass spectral characterization. Collision-induced fragmentation spectrometry identified characteristic fragment ions that were used to develop "rules" allowing assignment pattern as...
MAN1B1-CDG has recently been characterized as a type II congenital disorder of glycosylation (CDG), disrupting not only protein N-glycosylation but also general Golgi morphology. Using our high-throughput, quantitative ultra-performance liquid chromatography assay, we achieved detailed characterization the changes in both total serum glycoproteins and isolated IgG from ten previously reported patients. We have identified quantified novel hybrid high-mannosylated MAN1B1-CDG-specific glycans...
The direct association of the genome, transcriptome, metabolome, lipidome and proteome with serum glycome has revealed systems interconnected cellular pathways. exact roles individual glycoproteomes in context disease have yet to be elucidated. In a move toward personalized medicine, it is now becoming critical understand pathogenesis, traits, stages, phenotypes molecular features that accompany it, as disruption whole system. To this end, we developed an innovative technology on automated...
Classical Galactosaemia (CG) (OMIM #230400) is a rare inborn error of galactose metabolism caused by deficiency the enzyme galactose-1-phosphate uridylyltransferase (GALT). Long-term complications persist in treated patients despite dietary restriction with significant variations outcomes suggesting epigenetic glycosylation influences. Primary Ovarian Insufficiency (POI) very complication affecting females follicular depletion noted early life. We studied specific glycan synthesis, leptin...
Background Classical galactosaemia (OMIM #230400) is a rare disorder of carbohydrate metabolism caused by deficiency the galactose-1-phosphate uridyltransferase enzyme. The pathophysiology long-term complications, mainly cognitive, neurological and female fertility problems, remains poorly understood. Current clinical methods biochemical monitoring lack precision individualization with an identified need for improved biomarkers this condition. Methods We report development detailed...
Here we report evidence that new aminoquinoline N-glycan fluorescent labels interfere with the release of core α(1-6) fucose from N-glycans by bovine kidney α-l-fucosidase (BKF). BKF is a commonly employed exoglycosidase for determination. Molecular simulations bound and unbound Fuc-α(1-6)-GlcNAc, where GlcNAc situated at reducing end all N-glycans, suggest reduced activity may be due to nonoptimal fit highest populated conformers in active binding site room temperature. Population analysis...
High-throughput methods to identify and quantify glycans in a given sample are rare. We have optimised robotic platform for analysing biopharmaceuticals at each stage of the manufacturing process. In addition, it can be applied basic research. The plate format makes convenient large sets; is relatively cheap, robust quantitative. However, datasets churned out by this require significant time interpret. Consequently, informatics tool required help with annotation. This article briefly...
Previous studies have identified many immune pathways which are consistently altered in humans and model organisms as they age. Dairy cows often culled at quite young ages due to an inability cope adequately with metabolic infectious diseases, resulting reduced milk production infertility. Improved longevity is therefore a desirable trait would benefit both farmers their cows. This study analysed the transcriptome derived from RNA-seq data of leukocytes obtained Holstein early lactation...
Background and aim: Glycomic alterations serve as biomarker tools for different diseases. The present study aims to evaluate the diagnostic capability of serum N-glycosylation identify alcohol risk drinking in comparison with standard markers. Methods: We included 1516 adult individuals (age range 18-91 years; 55.3% women), randomly selected from a general population. A total 143 (21.0%) men 50 (5.9%) women were classified drinkers after quantification daily consumption Alcohol Use Disorders...