Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA currently used as marker of carcinoma because high levels are indicative tumor situation. However, tests still suffer from lack specificity to distinguish between benign hyperplasia and cancer. To determine whether glycosylation could provide means differentiating normal origins, N-glycan characterization seminal fluid cancer cells (LNCaP cell line) sequencing analysis mass spectrometry was carried...

Glycosylation of acute-phase proteins (APP), which is partially regulated by cytokines, may be distinct in disease and provide useful tumour markers. Thus, we have examined the glycosylation major serum APP pancreatic cancer (PaC), chronic pancreatitis (CP) control patients.Using a specific anti-sialyl Lewis X antibody N-glycan sequencing, determined changes on α-1-acid glycoprotein (AGP), haptoglobin (HPT), fetuin (FET), α-1-antitrypsin (AT) transferrin (TRF).Increased levels sialyl (SLe(x)...

New markers based on PSA isoforms have recently been developed to improve prostate cancer (PCa) diagnosis. However, novel approaches are still required differentiate aggressive from non-aggressive PCa decision making for patients. glycoforms shown be differentially expressed in PCa. In particular, changes the extent of core fucosylation and sialylation N-glycans patients compared healthy controls or BPH reported. The objective this study was determine these specific glycan structures serum...

Prostate-specific antigen (PSA), the tumor marker currently used for prostate cancer (PCa), is not specific enough to distinguish between PCa and benign hyperplasia (BPH). Glycan processing normally perturbed in tumors, therefore we investigated whether changes glycosylation of PSA could be useful diagnostic indicators. Previously determined that secreted by cell line LNCaP differs significantly from seminal plasma (normal control). We undertook a detailed glycan analysis derived sera...

Human pancreatic ribonuclease 1 (RNase 1) is a glycoprotein expressed mainly by the pancreas and also found in endothelial cells. The diagnosis of cancer (PaC) remains difficult therefore search for sensitive specific markers required. Previous studies showed that RNase from human healthy contained only neutral glycans, whereas PaC cell lines sialylated structures. To determine whether these glycan tumor cell-associated changes were characteristic serum could be used as marker PaC, we have...

Background Cell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation sialylated tumour antigens. We have focused on influence alpha-2,3-sialyltransferase ST3Gal III key steps pancreatic tumorigenic process. Methodology/Principal Findings overexpressing adenocarcinoma lines Capan-1 MDAPanc-28 were generated. They showed increase associated antigen...

In our previous studies we have described that ST3Gal III transfected pancreatic adenocarcinoma Capan-1 and MDAPanc-28 cells show increased membrane expression levels of sialyl-Lewis x (SLex) along with a concomitant decrease in α2,6-sialic acid compared to control cells. Here addressed the role this glycosylation pattern functional properties two glycoproteins involved processes cancer cell invasion migration, α2β1 integrin, main receptor for type 1 collagen, E-cadherin, responsible...

Prostate Specific Antigen (PSA) is the most commonly used serum marker for prostate cancer (PCa), although it not specific and sensitive enough to allow differential diagnosis of more aggressive tumors. For that, new diagnostic methods are being developed, such as PCA-3, PSA isoforms that have resulted in 4K score or Health Index (PHI), glycoforms. In present study, we compared PHI with our recently developed glycoform assay, based on determination α2,3-sialic acid percentage (% α2,3-SA), a...

Interleukin (IL)-10 is a potent immunosuppressant of monocyte/macrophage function and may help control the inflammatory response induced by bacterial infection. To analyze whether IL-10 detectable in plasma patients with septic shock to evaluate its relationship endotoxin (lipopolysaccharide [LPS])-induced monocyte/macrophage-induced response, IL-10, tumor necrosis factor (TNF)-alpha, IL-1 beta, IL-6, IL-8, LPS, neopterin were studied 24 12 critically ill patients. Eighty-three percent 25%...

Prostate-specific antigen (PSA) two-dimensional electrophoresis (2-DE) subforms (F1–F5) have been described to be altered in prostate cancer (PCa) compared benign prostatic hyperplasia (BPH). To understand their molecular differences, characterization of these from PCa serum and seminal plasma, namely, at the glycan level, was performed. PSA 2-DE two samples plasma were analyzed by N-glycan sequencing using high-performance liquid chromatography (HPLC) combined with exoglycosidase array...

Glycosylation is the most common posttranslational modification of proteins and highly reflective changes in environment a cell. Epigenetic modifications to genome are stably transmitted daughter cells without requirement for genetic sequence alterations. Aberrant regulation both epigenetic programming glycosylation patterning integral aspects carcinogenesis. The objective this study was determine interplay between these two complex cellular processes. We demonstrate that global DNA...

Tumor cells modulate their extracellular matrix (ECM) adhesion and migration to become more metastatic. Moreover, they show an increase in sialic acid, which could have effect on ECM migration. This work describes the influence of pancreatic adenocarcinoma cell surface α2,3- α2,6-sialic acid determinants aforementioned processes.We characterized acids, sialyl-Lewis x levels integrin 2 lines, Capan-1 MDAPanc-28, grown at different densities, also ST3Gal III overexpressing cells, C31. We...

Aberrant sialylation is frequently found in pancreatic ductal adenocarcinoma (PDA). α2,3-Sialyltransferases (α2,3-STs) ST3GAL3 and ST3GAL4 are overexpressed PDA tissues responsible for increased biosynthesis of sialyl-Lewis (sLe) antigens, which play an important role metastasis. This study addresses the effect α2,3-STs knockdown on migratory invasive phenotype cells, E-selectin-dependent adhesion. Characterization cell sialome, fucosyltransferases involved sLe using a panel human cells...

Characterization of the N-glycans from human pancreatic ribonuclease (RNase 1) isolated healthy pancreas and adenocarcinoma tumor cells (Capan-1 MDAPanc-3) revealed completely different glycosylation patterns. RNase 1 contained neutral complex biantennary structures, with smaller amounts tri- tetraantennary compounds, glycans poly-N-acetyllactosamine extensions, all extensively fucosylated. In contrast, (Capan-1) were fucosylated hybrid GalNAc-GlcNAc antennae. Capan-1 MDAPanc-3 also...

Abstract 2‐DE is broadly used for quantitative analysis of differential protein expression in complex mixtures such as serum samples or cell lysates. PTMs directly influence the pattern, and knowledge rules separation required order to understand distribution a gel. Glycosylation most common PTM can modify both molecular weight p I protein. In particular, effect charged monosaccharides (mainly sialic acids, SAs) on pattern major interest since changes sialylation are regularly observed...

Ovarian cancer is the most lethal gynaecological and often diagnosed in late stage, as result of unavailability sufficiently sensitive biomarkers for early detection, tumour progression tumour-associated inflammation. Glycosylation common posttranslational modification proteins; it altered therefore a potential source biomarkers. We investigated quantitative qualitative effects anti-inflammatory (acetylsalicylic acid) pro-inflammatory (thioglycolate chlorite-oxidized oxyamylose) drugs on...