A5010441434- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Protease and Inhibitor Mechanisms
- Ocular Diseases and Behçet’s Syndrome
- Proteoglycans and glycosaminoglycans research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- interferon and immune responses
- Retinal and Optic Conditions
- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- S100 Proteins and Annexins
- Drug-Induced Hepatotoxicity and Protection
- Eosinophilic Esophagitis
- Immune responses and vaccinations
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Lymphocytic Leukemia Research
- Neonatal Respiratory Health Research
- Neuropeptides and Animal Physiology
- Macrophage Migration Inhibitory Factor
Rega Institute for Medical Research
2016-2025
KU Leuven
2016-2025
Infection, sterile injury and chronic inflammation trigger the acute phase response in order to re-establish homeostasis. This includes production of positive proteins liver, such as members serum amyloid A (SAA) family. In humans major SAAs comprise a group closely related variants SAA1 SAA2. was proven be chemotactic for several leukocyte subtypes through activation G protein-coupled receptor FPRL1/FPR2. Several other biological activities SAA1, cytokine induction, reported mediated via...
Abstract Cell migration depends on the ability of leukocytes to sense an external gradient chemotactic proteins produced during inflammation. These include chemokines, complement factors, and some acute phase proteins, such as serum amyloid A. Serum A chemoattracts neutrophils, monocytes, T lymphocytes via its G protein-coupled receptor formyl peptide 2. We demonstrate that A1α more potently neutrophils in vivo than vitro. In contrast CD14+ no rapid (within 2 h) induction interleukin-8/CXC...
Abstract Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening immunological disorder that characterized by systemic inflammation, widespread organ damage, and hypercytokinemia. Primary HLH caused mutations in granule-mediated cytotoxicity, whereas secondary occurs, without known genetic background, context of infections, malignancies, or autoimmune autoinflammatory disorders. Clinical manifestations both subtypes are often precipitated viral infection, predominantly with...
Purpose: The purpose of this study was to determine levels the cytokines IL-1β, IL-6, IL-21, IL-22, and IL-23 chemokines CXCL13, CCL19, CCL20, CCL21 in aqueous humor (AH) samples from patients with specific uveitic entities. Methods: Paired serum (n = 13) AH 111) active idiopathic granulomatous uveitis (IGU) or associated HLA-B27-related inflammation, Behçet's disease (BD), Vogt-Koyanagi-Harada (VKH) disease, sarcoidosis control were analyzed two different multiplex assays. Results:...
One of the most important functions chemokines and their receptors is regulation directional migration leukocytes within tissues. In specific tissue compartments, cells are exposed to multiple presented in complex dimensional temporal patterns. Therefore, a leukocyte requires mechanisms integrate various signals it receives from different chemoattractants. this study, we report that CCL3, CCL5, CCL8, three potent mononuclear cell chemoattractants, able synergize with homeostatic chemokine...
Ovarian cancer is the most lethal gynaecological and often diagnosed in late stage, as result of unavailability sufficiently sensitive biomarkers for early detection, tumour progression tumour-associated inflammation. Glycosylation common posttranslational modification proteins; it altered therefore a potential source biomarkers. We investigated quantitative qualitative effects anti-inflammatory (acetylsalicylic acid) pro-inflammatory (thioglycolate chlorite-oxidized oxyamylose) drugs on...
Purpose: To determine the levels of neutrophil chemoattractants CXCL1, CXCL2, CXCL5, CXCL6, and CXCL8, T helper 1 CXCL9, CXCL10 CXCL11, lymphoid chemokines CXCL12 CXCL13 soluble form transmembrane CXCL16 CX3CL1, in aqueous humor samples from patients with specific uveitic entities. Methods: Aqueous active uveitis associated Behçet's disease (n = 13), sarcoidosis 8), HLA-B27–related inflammation 12), Vogt-Koyanagi-Harada (VKH) healthy controls 9) were assayed use a multiplex assay. Results:...
Several acute and chronic inflammatory diseases are driven by accumulation of activated leukocytes due to enhanced chemokine expression. In addition specific G protein-coupled receptor (GPCR)-dependent signaling, – glycosaminoglycan (GAG) interactions important for activity in vivo. Therefore, the GAG interaction has been explored as target inhibition activity. It was demonstrated that CXCL9(74-103) binds with high affinity GAGs, competed active chemokines binding thereby inhibited CXCL8-...
Abstract Systemic juvenile idiopathic arthritis (sJIA) is a childhood-onset immune disorder of unknown cause. One the concepts that disease results from an inappropriate control responses to initially harmless trigger. In current study, we investigated whether sJIA may be caused by defects in IL-10, key cytokine controlling inflammation. We used translational approach, with sJIA-like mouse model and patient samples. The relies on injection CFA IFN-γ–deficient BALB/c mice; corresponding wild...
Purpose: To evaluate expression of cytokines GM-CSF, IL-11, IL-12p40, IL-12p70, IL-27p28, IL-35, APRIL, BAFF, TWEAK, and LIGHT in uveitis.Methods: Aqueous humor samples from patients with active uveitis associated Behçet's disease (BD), sarcoidosis, HLA-B27-related inflammation, Vogt-Koyanagi-Harada (VKH) control were assayed a multiplex assay.Results: Comparing all to controls, BAFF significantly increased, whereas was decreased. IL-11 the most strongly upregulated, being elevated 19.7-fold...
Necrotic cell death causes loss of membrane integrity, release intracellular contents and deposition necrotic debris. Effective clearance this debris is crucial for resolving inflammation promoting tissue recovery. While leukocyte phagocytosis plays a major role, soluble factors in the bloodstream also contribute to removal. Our study examined whether enzymatic degradation or disassembly enhances improves outcomes mouse model drug-induced liver injury. Using intravital microscopy proteomic...
Evidence exists that pentraxin 3 (PTX3) is involved in the pathogenesis of autoimmune diseases. The aim this study was to analyze levels PTX3 aqueous humor (AH) samples from patients with specific uveitic entities. In addition, we correlated proinflammatory cytokines and chemokines clinical disease activity. AH active uveitis associated human leukocyte antigen (HLA)-B27-related inflammation (n = 12), Behçet's (BD) 13), Vogt-Koyanagi-Harada (VKH) sarcoidosis 8) control subjects 9) were...
Usutu virus (USUV) is an emerging neurotropic flavivirus capable of causing encephalitis in humans. Here, our main goal was to characterize the innate immune response brain during USUV and identify strategies control disease severity. Using immunocompetent mouse model encephalitis, we showed that microglia activation, blood-brain barrier (BBB) disruption inflammatory monocyte recruitment are hallmarks 6 days post infection. Activated were close association USUV-infected cells, concomitantly...
ABSTRACT Nonspecific binding of monoclonal antibodies to Fcγ receptors (FcγRs) is a well‐known root cause unreliable results in flow cytometry. Over the past decade, liver Group 1 innate lymphoid cells (ILCs), including conventional natural killer (cNK) and type ILCs (ILC1s), have been extensively studied by cytometry various inflammatory disorders. In our previous work, we specifically evaluated changes ILC1 numbers two murine models Toll‐like receptor (TLR)‐induced macrophage activation...
Necrotic cell death causes loss of membrane integrity, release intracellular contents and deposition necrotic debris. Effective clearance this debris is crucial for resolving inflammation promoting tissue recovery. While leukocyte phagocytosis plays a major role, soluble factors in the bloodstream also contribute to removal. Our study examined whether enzymatic degradation or disassembly enhances improves outcomes mouse model drug-induced liver injury. Using intravital microscopy on-tissue...
Posttranslational modification of chemokines is one the mechanisms that regulate leukocyte migration during inflammation. Multiple natural NH(2)-terminally truncated forms major human neutrophil attractant interleukin-8 or CXCL8 have been identified. Although differential activity was reported for some forms, no biological data are available others.Aminopeptidase-cleaved CXCL8(2-77) and CXCL8(3-77), product alternative cleavage signal peptide CXCL8(-2-77) previously studied containing 77 72...
Abstract: The need for test systems nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological chemical contaminants, devoid toxicity. Nevertheless, in the absence contamination, these particles may still induce undesired effects vivo, such as enhanced autoimmunity, hypersensitivity reactions, fibrosis. Here we show that artificial specific sizes affect immune cell recruitment tested a dermal air...
// Katrien Van Raemdonck 1 , Nele Berghmans Vincent Vanheule Antonella Bugatti 2 Paul Proost Ghislain Opdenakker 3 Marco Presta Jo Damme Sofie Struyf Laboratory of Molecular Immunology, KU Leuven - University Leuven, Department Microbiology and Rega Institute for Medical Research, Belgium Experimental Oncology Brescia, Translational Medicine Italy Immunobiology, Correspondence to: Struyf, e-mail: Sofie.Struyf@rega.kuleuven.be Keywords: chemokines, CXCL4, CXCL4L1, EGF, CCL5, angiogenesis...
Abstract CXCL8 is the principal human neutrophil-attracting chemokine and a major mediator of inflammation. The exerts its neutrophil-chemotactic neutrophil-activating activities via interaction with glycosaminoglycans (GAGs) activation G protein-coupled receptors (GPCRs) CXCR1 CXCR2. Natural displays an exceptional degree amino (NH2)-terminal heterogeneity. Most forms result from proteolytic processing authentic CXCL8(1-77). Here, we compared potencies to activate recruit neutrophils 3 most...
To determine the concentrations of CC chemokines CCL2, CCL7, CCL8, CCL11, CCL13, CCL20, CCL24 and CCL26 in aqueous humour (AH) samples from patients with specific uveitic entities.Aqueous active uveitis associated Behçet's disease (BD) (n = 13), sarcoidosis 8), HLA-B27-related inflammation 12), Vogt-Koyanagi-Harada (VKH) 12) control 9) were assayed use a multiplex assay.When considering all as one group, chemokine levels except CCL2 significantly increased compared to controls. CCL13 CCL20...
Growth factors such as vascular endothelial growth factor (VEGF), fibroblast (FGF) and epidermal (EGF) are important angiogenesis-mediating factors. They exert their effects not only through respective receptor tyrosine kinases (RTKs), but they also require molecular pairing with heparan sulfate proteoglycans (HSPGs). Angiogenic signaling pathways commonly targeted in current anti-angiogenic cancer therapies have unfortunately insufficient impact on patient survival. Considering obvious role...