A5010791500- Chemokine receptors and signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Immune cells in cancer
- Inflammasome and immune disorders
- Cell Adhesion Molecules Research
- COVID-19 Clinical Research Studies
- Protease and Inhibitor Mechanisms
- Olfactory and Sensory Function Studies
- Antimicrobial Peptides and Activities
- S100 Proteins and Annexins
- Blood disorders and treatments
- T-cell and B-cell Immunology
- Nicotinic Acetylcholine Receptors Study
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Cystic Fibrosis Research Advances
- Neuropeptides and Animal Physiology
- T-cell and Retrovirus Studies
- Autoimmune and Inflammatory Disorders Research
- Sarcoidosis and Beryllium Toxicity Research
- Macrophage Migration Inhibitory Factor
- interferon and immune responses
- Sepsis Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
Rega Institute for Medical Research
2016-2024
KU Leuven
2016-2024
Erasmus MC
2023
Humanitas University
2021
Istituti di Ricovero e Cura a Carattere Scientifico
2021
Neutrophils are the most abundant leukocytes in human blood and first cells responding to infection injury. Due their limited ex vivo lifespan impossibility cryopreserve or expand them vitro , neutrophils need be purified from fresh for immediate use experiments. Importantly, neutrophil purification methods may artificially modify phenotype functional characteristics of isolated cells. The aim this study was expose effects ‘classical’ density-gradient versus more expensive but faster...
Neutrophils are recognized as important circulating effector cells in the pathophysiology of severe coronavirus disease 2019 (COVID-19). However, their role within inflamed lungs is incompletely understood. Here, we collected bronchoalveolar lavage (BAL) fluids and parallel blood samples critically ill COVID-19 patients requiring invasive mechanical ventilation compared BAL fluid parameters with those mechanically ventilated influenza, a non-COVID-19 viral pneumonia cohort. Compared...
Abstract Objectives Emerging evidence of dysregulation the myeloid cell compartment urges investigations on neutrophil characteristics in coronavirus disease 2019 (COVID‐19). We isolated neutrophils from blood COVID‐19 patients receiving general ward care and hospitalised at intensive units (ICUs) to explore kinetics circulating factors important for migration activation. Methods Multicolour flow cytometry was exploited analysis differentiation activation markers. Multiplex ELISA...
BackgroundThe molecular cause of severe congenital neutropenia (SCN) is unknown in 30% to 50% patients. SEC61A1 encodes the α-subunit Sec61 complex, which governs endoplasmic reticulum protein transport and passive calcium leakage. Recently, mutations were reported be pathogenic common variable immunodeficiency glomerulocystic kidney disease.ObjectiveOur aim was expand spectrum SEC61A1-mediated disease include autosomal dominant SCN.MethodsWhole exome sequencing findings validated, compared...
Reflecting their importance in immunity, the activity of chemokines is regulated on several levels, including tissue and context-specific expression availability cognate receptor target cells. Chemokine synergism, affecting both chemokine function, has emerged as an additional control mechanism. We previously demonstrated that CXCL14 a positive allosteric modulator CXCR4 its ability to synergise with CXCL12 diverse cellular responses. Here, we have extended our study homeostatic, well...
CXC chemokine ligand (CXCL)9, CXCL10 and CXCL11 direct chemotaxis of mainly T cells NK through activation their common receptor (CXCR)3. They are inactivated upon NH₂-terminal cleavage by dipeptidyl peptidase IV/CD26. In the present study, we found that different glycosaminoglycans (GAGs) protect CXCR3 ligands against proteolytic processing CD26 without directly affecting enzymatic activity CD26. addition, GAGs were shown to interfere with chemokine-induced signaling. The observation heparan...
Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in childhood. The predominant subtypes, oligoarticular and polyarticular JIA, are traditionally considered to be autoimmune diseases with a central role for T cells autoantibodies. Mounting evidence suggests an important neutrophils JIA pathogenesis. We undertook this study investigate phenotypic features of present blood inflamed joints patients.JIA synovial fluid (SF) parallel samples from patients healthy children...
Abstract CXCL8 is the principal human neutrophil-attracting chemokine and a major mediator of inflammation. The exerts its neutrophil-chemotactic neutrophil-activating activities via interaction with glycosaminoglycans (GAGs) activation G protein-coupled receptors (GPCRs) CXCR1 CXCR2. Natural displays an exceptional degree amino (NH2)-terminal heterogeneity. Most forms result from proteolytic processing authentic CXCL8(1-77). Here, we compared potencies to activate recruit neutrophils 3 most...
CXCL8 or interleukin (IL)-8 directs neutrophil migration and activation through interaction with CXCR1 CXCR2 that belong to the family of G protein-coupled receptors (GPCRs). Naturally occurring posttranslational modifications NH2-terminal region affect its biological activities, but underlying molecular mechanisms are only partially understood. Here, we studied implications site-specific citrullination truncation for signaling potency CXCL8. Native CXCL8(1-77), citrullinated...
Proteolytic processing of CXCL5 potentiates its chemoattractant activity toward neutrophils and monocytes in vitro vivo.
Introduction Peptidylarginine deiminases (PADs) mediate citrullination, an irreversible posttranslational modification that converts arginine to citrulline residues in proteins. Rheumatoid arthritis (RA) is characterized by unique autoantibodies recognize citrullinated peptides, which are highly specific for this disease. However, the mechanism preceding anti-citrulline response remains largely unclear. PAD enzymes known fuel autoimmune generating autoreactive epitopes, and sustain local...
With ELISAs one detects the ensemble of immunoreactive molecules in biological samples. For biomolecules undergoing proteolysis for activation, potentiation or inhibition, other techniques are necessary to study biology. Here we develop methodology that combines immunosorbent sample preparation and nano-scale liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) proteoform analysis (ISTAMPA) apply this aglycosyl chemokine CXCL8. CXCL8, most powerful human with neutrophil chemotactic...
Abstract Background Chemical communication is an important aspect of the behavioural ecology a wide range mammals. In dogs and other carnivores, anal sac glands are thought to convey information conspecifics by secreting pallet small volatile molecules produced symbiotic bacteria. Because these unique it unclear how their secretions relate those placental mammals that make use different tissues for chemical communication. Here we analyse domestic verify secretion proteins infer evolutionary...
Elevated neutrophil counts in broncho-alveolar lavage (BAL) fluids of lung transplant (LTx) patients with chronic allograft dysfunction (CLAD) are associated disease pathology. However, phenotypical characteristics these cells remained largely unknown. Moreover, despite enhanced levels the most potent human neutrophil-attracting chemokine CXCL8 BAL fluid, no discrimination had been made between natural NH2-terminally truncated proteoforms, which exhibit up to 30-fold differences biological...
Interleukin 7 (IL-7) is a cell growth factor with central role in normal T development, survival and differentiation. The lack of IL-7–IL-7 receptor(R)-mediated signaling compromises lymphoid whereas increased activity contributes to the development chronic inflammation, cancer autoimmunity. Gain-of-function alterations IL-7R through Janus kinases (JAKs) signal transducers activators transcription (STATs) are enriched acute lymphoblastic leukemia (T-ALL) autocrine production IL-7 by T-ALL...
Abstract The nasal and bronchial epithelium are unified parts of the respiratory tract that affected in monogenic disorder cystic fibrosis (CF). Recent studies have uncovered tissues exhibit intrinsic variability, including differences mucociliary cell composition expression unique transcriptional regulatory proteins which relate to germ layer origin. In present study, we explored whether between epithelial cells persist cultures affect functioning CF. Comparison air–liquid interface (ALI)...