A5077706372- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Sphingolipid Metabolism and Signaling
- Lymphatic System and Diseases
- HIV Research and Treatment
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Inflammatory mediators and NSAID effects
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- Caveolin-1 and cellular processes
- Signaling Pathways in Disease
- Cancer Cells and Metastasis
- Platelet Disorders and Treatments
- Galectins and Cancer Biology
- Cellular Mechanics and Interactions
- interferon and immune responses
University of Bern
1996-2025
Biotechnology Institute Thurgau
2016-2025
University of Konstanz
2016-2025
Institute of Cell Biology
2024
Università della Svizzera italiana
2017
Medizinische Hochschule Hannover
2010
Aichi Cancer Center
2010
Nagoya Industrial Science Research Institute
2010
University of Bonn
2010
Hanson Institute
2010
Although most leukocytes, T lymphocytes in particular, respond to several different chemokines, there is virtually no information on chemokine activities and receptors B lymphocytes. A putative receptor, BLR1, that expressed Burkitt's lymphoma cells was cloned a few years ago. Deletion of the gene for BLR1 yielded mice with abnormal primary follicles germinal centers spleen Peyer's patches, reflecting inability migrate into cell areas. By screening sequence tag DNA sequences, we have...
Directional guidance of cells via gradients chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous chemokine CCL21 within mouse skin show that they guide dendritic toward lymphatic vessels. Quantitative imaging reveals depots endothelial steeply decaying perilymphatic interstitium. These match migratory patterns cells, which...
Secondary lymphoid-tissue chemokine, SLC, also known as exodus-2 and 6Ckine, is a novel CC chemokine with selectivity for T lymphocytes preferential expression in lymphoid tissues. We have studied its production, receptor usage biological activities. High levels of SLC mRNA were detected lymph nodes, the gastrointestinal tract several gland tissues, but no was found by Northern blot analysis freshly isolated or stimulated blood monocytes lymphocytes, neutrophils eosinophils. In situ...
Abstract The control of dendritic cell (DC) migration is pivotal for the initiation cellular immune responses. In this study, we demonstrate that human monocyte-derived (Mo)DCs as well ex vivo peripheral blood DCs toward CCL21, CXCL12, and C5a stringently dependent on presence proinflammatory mediator PGE2, although expressed CXCR4 C5aR their surface DC maturation was accompanied by CCR7 up-regulation independently PGE2. necessity exogenous PGE2 not due to suppression synthesis IL-4, which...
The nucleotide sequence for a putative chemokine receptor, termed TER1, ChemR1, or CKR-L1, was recently obtained by polymerase chain reaction-based cloning technique. It encodes protein of 355 amino acids that shows 32–45% identity with human receptors. gene localized on chromosome 3p21–24, the site genes five known CC receptors, suggesting natural ligand may be chemokine. We have stably expressed this receptor in murine pre-B cells 300-19 and tested their responsiveness to 20 chemokines...
Abstract Through the formation of concentration gradients, morphogens drive graded responses to extracellular signals, thereby fine-tuning cell behaviors in complex tissues. Here we show that chemokine CXCL13 forms both soluble and immobilized gradients. Specifically, + follicular reticular cells form a small-world network guidance structures, with computer simulations optimization analysis predicting gradients created by this promote B trafficking. Consistent prediction, imaging binds...
ABSTRACT The cellular prion protein (PrP c ) resides in lipid rafts, yet the type of raft and physiological function PrP are unclear. We show here that cross‐linking with specific antibodies leads to 1) capping Jurkat human peripheral blood T cells; 2) cocapping intracellular proteins reggie‐1 reggie‐2; 3) signal transduction as seen by MAP kinase phosphorylation an elevation Ca 2+ concentration; 4) recruitment Thy‐1, TCR/CD3, fyn, lck LAT into cap along local tyrosine F‐actin...
Abstract The chemokine receptor CCR7 and its ligands CCL19 CCL21 play a crucial role for the homing of lymphocytes dendritic cells to secondary lymphoid tissues. Nevertheless, how senses gradient chemokines migration is terminated are poorly understood. In this study, we demonstrate that CCR7(-GFP) endocytosed into early endosomes containing transferrin upon binding, but less triggering. Internalization was independent lipid rafts relied on dynamin Eps15 inhibited by hypertonic sucrose,...
Partitioning of proteins in cholesterol and sphingolipid enriched plasma membrane microdomains, called lipid rafts, is critical for many signal transduction protein sorting events.Although raft partitioning signaling molecules remains to be determined, glycosylphosphatidyl-inositol (GPI)-anchored possess high affinity rafts are currently exploited as markers investigate fundamental mechanisms events.In this study, we demonstrate that two recombinant GPI-anchored green fluorescent (GFP-GPIs)...
<ns4:p>Chemokine signaling is essential for coordinated cell migration in health and disease to specifically govern positioning space time. Typically, chemokines signal through heptahelical, G protein-coupled receptors orchestrate migration. Notably, chemokine are highly dynamic structures efficiency largely depends on the discrete contact with ligand. Promiscuity of both receptors, combined biased allosteric modulation receptor activation, guarantees a tightly controlled recruitment...