A5056447944- Mesenchymal stem cell research
- Immune Cell Function and Interaction
- Advanced Memory and Neural Computing
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neural Engineering
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Nanowire Synthesis and Applications
- Immune cells in cancer
- CAR-T cell therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Fibroblast Growth Factor Research
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Mathematical Biology Tumor Growth
- Bioinformatics and Genomic Networks
- Galectins and Cancer Biology
- Alzheimer's disease research and treatments
- Carbohydrate Chemistry and Synthesis
- Salivary Gland Disorders and Functions
- Multiple Myeloma Research and Treatments
Ludwig Cancer Research
2001-2024
University of Lausanne
2014-2024
Ichnos Sciences (United States)
2023
Hôpital Orthopédique de la Suisse Romande
2016
Center for Cancer Research
2014
SIB Swiss Institute of Bioinformatics
2013
Medizin Campus Bodensee
2013
Cancer Research Center
2013
University Hospital of Lausanne
2006-2012
Swiss Institute of Comparative Law
2011
Abstract Expansion of antigen-experienced CD8 + T cells is critical for the success tumour-infiltrating lymphocyte (TIL)-adoptive cell therapy (ACT) in patients with cancer 1 . Interleukin-2 (IL-2) acts as a key regulator cytotoxic functions by promoting expansion and capability 2,3 Therefore, it essential to comprehend mechanistic barriers IL-2 sensing tumour microenvironment implement strategies reinvigorate responsiveness antitumour responses. Here we report that prostaglandin E2 (PGE 2...
C-mannosylation of Trp-7 in human ribonuclease 2 (RNase 2) is a novel kind protein glycosylation that differs fundamentally from N- and O-glycosylation the protein-sugar linkage. Previously, we established specificity determinant acceptor substrate consists sequence -x-x-W, where first Trp becomes C-mannosylated. Here investigated reaction with respect to mannosyl donor involvement glycosyltransferase. was reduced 10-fold CHO (Chinese hamster ovary) Lec15 cells, which are deficient...
Immunotherapy is facing a revolution with the advent of immune cell engineering. Chimeric antigen receptor (CAR)-T therapy has shown unprecedented efficacy in B malignancies and now being evaluated other disease areas. Viral transduction most common method for genetic engineering, but presents important limitations, such as high reagent costs regulatory concerns due to mutagenesis risk. One prevailing non-viral gene delivery strategy relies on electroporation non-integrating RNA. However,...
Partitioning of proteins in cholesterol and sphingolipid enriched plasma membrane microdomains, called lipid rafts, is critical for many signal transduction protein sorting events.Although raft partitioning signaling molecules remains to be determined, glycosylphosphatidyl-inositol (GPI)-anchored possess high affinity rafts are currently exploited as markers investigate fundamental mechanisms events.In this study, we demonstrate that two recombinant GPI-anchored green fluorescent (GFP-GPIs)...
Redirecting CD8 T cell immunity with self/tumor-specific affinity-matured TCRs is a promising approach for clinical adoptive therapy, the aim to improve treatment efficacy. Despite numerous functional-based studies, little known about characteristics of TCR signaling (i.e. intensity, duration and amplification) regulatory mechanisms underlying optimal therapeutic responses. Using panel human SUP-T1 primary cells engineered incremental affinity against cancer-testis antigen NY-ESO-1, we found...
Tumor-associated macrophages (TAM) are well known as a key player in the tumor microenvironment, which support cancer progression. More recently, lineage of monocytes characterized by expression TIE-2/Tek angiopoietin receptor identified subset circulating and tumor-associated endowed with proangiogenic activity. TIE-2 expressing (TEM) were found both humans mice. Here, we review phenotypes functions TEM reported so far human their potential use markers progression metastasis. Finally,...
The Lec35 gene product (Lec35p) is required for utilization of the mannose donor mannose-P-dolichol (MPD) in synthesis both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols, which are important functions such as protein folding membrane anchoring, respectively. hamster shown to encode previously identified cDNA SL15, corrects mutant phenotype predicts a novel endoplasmic reticulum protein. alleles Lec35.1 Lec35.2 characterized, human (mannose-P-dolichol defect 1) was...
In experimental mouse models of cancer, increasingly compelling evidence point toward a contribution tumor associated macrophages (TAM) to lymphangiogenesis. Corresponding observations in human cancer remain scarce although lymphatic metastasis is widely recognized as predominant route for spread. We previously showed that, malignant tumors untreated breast (BC) patients, TIE-2-expressing monocytes (TEM) are highly proangiogenic immunosuppressive cells and that TIE-2 VEGFR signaling pathways...
We attempt to examine the potential of silicon nanowire memristors in field nanobiosensing. The memristive devices are crystalline Silicon (Si) Nanowires (NWs) with Nickel Silicide (NiSi) terminals. nanowires fabricated on a Silicon-on-Insulator (SOI) wafer by an Ebeam Lithography Technique (EBL) process that allows high resolution at nanoscale. A Deep Reactive Ion Etching (DRIE) technique is used define free-standing nanowires. close alignment between and Nickel-Silicide terminals forms...
Abstract Avidity of Ag recognition by tumor-specific T cells is one the main parameters that determines potency a tumor rejection Ag. In this study we show relative efficiency staining Ag-specific lymphocytes with corresponding fluorescent MHC class I/peptide multimeric complexes can considerably vary conditions and does not necessarily correlate avidity recognition. Instead, found clear correlation between stability interaction TCR as measured in dissociation kinetic experiments. These...
The β-chain of human interleukin 12 (IL-12) contains at position 319–322, the sequence Trp-x-x-Trp. In RNase 2 this is recognition motif for a new, recently discovered posttranslational modification, i.e., C-glycosidic attachment mannosyl residue to side chain tryptophan. Analysis C-terminal peptides recombinant IL-12 (rHuIL-12) by mass spectrometry and NMR spectroscopy revealed that Trp-319β (partially) C-mannosylated. This finding was extended in vitro mannosylation experiments, using...
C 2-α-Mannosyltryptophan was discovered in RNase 2 from human urine, representing a novel way of attaching carbohydrate to protein. Here, we have addressed two questions related the biosynthesis this modification: (i) isC-mannosylation part normal intracellular biosynthetic route, and (ii) how general is it, i.e. which organisms perform kind glycosylation? To answer first question, 2, identical eosinophil-derived neurotoxin, isolated stores cultured HL-60 cells. The enzyme C-mannosylated at...
Recently, the C-mannosylation of a specific tryptophan residue in RNase 2 from human urine has been reported [Hofsteenge, J., et al. (1994) Biochemistry 33, 13524−13530; de Beer, T., (1995) 34, 11785−11789]. In those studies, identification this unusual modification was accomplished by mass spectrometric and NMR spectroscopic analysis peptide fragments. The evidence for occurrence C2-α-mannosyltryptophan [(C2-Man-)Trp] intact protein relied exclusively on detection same phenylthiohydantoin...
Recognition by CD8+ cytotoxic T lymphocytes (CTLs) of antigenic peptides bound to major histocompatibility class (MHC) I molecules on target cells leads sustained calcium mobilization and CTL degranulation resulting in perforin-dependent killing. We report that β1 β3 integrin-mediated adhesion extracellular matrix proteins and/or surfaces dramatically promotes degranulation. CTLs, when adhered fibronectin but not suspension, efficiently degranulate upon exposure soluble MHC·peptide...
This article presents electrically-based sensors made of high quality silicon nanowire field effect transistors (SiNW-FETs) for sensitive detection vascular endothelial growth factor (VEGF) molecules. SiNW-FET devices, fabricated through an IC/CMOS compatible top-down approach, are covalently functionalized with VEGF monoclonal antibodies in order to sense VEGF. Increasing concentrations the femto molar range determine increasing conductance values as proof occurring immuno-reactions at (NW)...