A5066138864- Liver physiology and pathology
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Chemokine receptors and signaling
- Inflammatory mediators and NSAID effects
- Cancer Mechanisms and Therapy
- Immune cells in cancer
- Brain Metastases and Treatment
- Liver Disease Diagnosis and Treatment
- Inflammasome and immune disorders
- Cardiovascular Disease and Adiposity
- Atherosclerosis and Cardiovascular Diseases
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
George Washington University
2019-2024
Universidad San Pablo CEU
2010-2020
Valencia Catholic University Saint Vincent Martyr
2017
Universitat de València
2017
University of the Basque Country
2002-2013
National Institutes of Health
2012
Tufts University
2012
Science Applications International Corporation (United States)
2012
National Cancer Institute
2012
University School
2012
Liver metastases present with distinct histopathological growth patterns (HGPs), including the desmoplastic, pushing and replacement HGPs two rarer HGPs. The are defined owing to interface between cancer cells adjacent normal liver parenchyma that is in each pattern can be scored from standard haematoxylin-and-eosin-stained (H&E) tissue sections. current study provides consensus guidelines for scoring these Guidelines defining were established by a large international team. To assess...
Proinflammatory cytokines, including IL-1beta and tumor necrosis factor-alpha (TNF-alpha), promote cancer cell adhesion liver metastases by up-regulating the expression of vascular molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE). In this study, metastasis after intrasplenically injected mouse B16 melanoma (B16M) cells was reduced 84-95% in mice with null mutations for either or IL-1beta-converting enzyme (ICE, caspase-1) compared wild-type mice. On day 12, 47% were dead 19%...
Myofibroblasts infiltrate malignant liver tumors, although their pathogenic implications are unclear. Immunohistochemical detection of α–smooth muscle actin, glial fibrillary acidic protein (GFAP), and CD31 CD34 expression was used to analyze the contribution myofibroblasts angiogenesis in hepatic metastasis produced by intrasplenically–injected B16 melanoma (B16M). Because activated stellate cells (HSCs) oxygen–sensing producing vascular endothelial growth factor (VEGF), effect B16M human...
BackgroundPrevious studies identified the human nonmetastatic gene 23 (NME1, hereafter Nm23-H1) as first metastasis suppressor gene. An inverse relationship between Nm23-H1 and expression of lysophosphatidic acid receptor 1 (LPAR1, also known EDG2 or LPA1) has been reported. However, effects LPA1 inhibition on primary tumor size, metastasis, metastatic dormancy have not investigated.
Using electron microscopy and cytofluorimetry we studied the role of carbohydrate-specific recognition systems in interaction apoptotic bodies with normal interleukin 1-activated sinusoidal endothelial cells. Microfluorimetric observation liver tissue sections revealed octadecylrhodamine B-labelled body binding to wall mouse liver, when they were injected intraportally. Plate-scanning demonstrated that about 20-25% Acridine Orange-labelled could adhere specifically cultured cells after 15...
In this work we report the presence of intrametastatic smooth-muscle iso-α-actin (SMA)-expressing cells which appeared from early stages hepatic metastasis process intrasplenically injected B16 melanoma (B16M) cells. They formed a network stromal among B16M cells, very low percentage them expressing desmin. contrast, those parts liver tissue unaffected by had perisinusoidal desmin-expressing quiescent stellate (qHSC) did not express SMA. Exposure freshly isolated rat to cell-conditioned...
The liver is a major metastasis-susceptible site and majority of patients with hepatic metastasis die from the disease in absence efficient treatments. intrahepatic circulation microvascular arrest cancer cells trigger local inflammatory reaction leading to cell apoptosis cytotoxicity via oxidative stress mediators (mainly nitric oxide hydrogen peroxide) natural killer cells. However, certain that resist or even deactivate these anti-tumoral defense mechanisms still can adhere endothelial...
The growth of cancer cells in inflammatory tissue is often observed. This can be the result favorable conditions for endothelial cell adherence and/or increased production local factors. role proinflammatory cytokine interleukin 1 (IL-1) prometastatic and growth-promoting environment inflammation was studied vivo, mechanism action vitro as well. Systemic induced by intravenous injection IL-1β or lipopolysaccharide (LPS), hepatic metastasizing ability B16 melanoma (B16) following intrasplenic...
Background Cell surface sialylation is emerging as an important feature of cancer cell metastasis. Sialyltransferase expression has been reported to be altered in tumours and may account for the formation sialylated tumour antigens. We have focused on influence alpha-2,3-sialyltransferase ST3Gal III key steps pancreatic tumorigenic process. Methodology/Principal Findings overexpressing adenocarcinoma lines Capan-1 MDAPanc-28 were generated. They showed increase associated antigen...
Mannose receptor (ManR)-mediated liver sinusoidal endothelial cell (LSEC) endocytosis plays a role in antigen presentation and innate immunity, but its hepatic metastasis is unknown. We studied ManR-mediated during C26 colorectal cancer interaction with LSECs implications metastasis. Uptake of labeled ManR ligands (mannan ovalbumin) immunohistochemistry were used to study expression. Several interleukin (IL)-1 inhibitors the cyclooxygenase-2 (COX-2) inhibitor celecoxib analyze IL-1 COX-2...
<h3>Background</h3> The transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts is a major mechanism for stroma development in metastasis, but their regulatory pathways remain unclear. Transdifferentiated HSCs from fibrotic liver express high levels the fibrillar collagen receptor discoidin domain 2 (DDR2), it unclear if DDR2 plays direct profibrogenic role tumour microenvironment. <h3>Aim</h3> To assess impact on prometastatic HSC-derived myofibroblasts. <h3>Methods</h3>...
Brain metastases occur in more than one-third of metastatic breast cancer patients whose tumors overexpress HER2 or are triple negative. colonization cells occurs a unique environment, containing microglia, oligodendrocytes, astrocytes, and neurons. Although neuroinflammatory response has been documented brain metastasis, its contribution to progression therapy remains poorly understood. Using an experimental metastasis model, we characterized the microenvironment tropic, HER2-transfected...
Abstract Background The recruitment of vascular stromal and endothelial cells is an early event occurring during cancer cell growth at premetastatic niches, but how the microenvironment created by initial three-dimensional (3D) affects their angiogenesis-stimulating potential unclear. Methods proangiogenic profile CT26 murine colorectal carcinoma was studied in seven-day cultured 3D-spheroids <300 μm diameter, produced hanging-drop method to mimic avascular micrometastases prior hypoxia...