A5074479868- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Angiogenesis and VEGF in Cancer
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Cutaneous lymphoproliferative disorders research
- Mesenchymal stem cell research
- Cancer Research and Treatments
- Advanced MRI Techniques and Applications
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Hematopoietic Stem Cell Transplantation
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Chronic Lymphocytic Leukemia Research
- Advanced biosensing and bioanalysis techniques
- TGF-β signaling in diseases
- Autophagy in Disease and Therapy
- Zeolite Catalysis and Synthesis
Institute of Cancer Research
2013-2023
Cancer Research UK
2014-2023
Imperial College London
2023
Royal Marsden NHS Foundation Trust
2014-2023
NIHR Imperial Biomedical Research Centre
2023
Engineering and Physical Sciences Research Council
2014
European Institute of Oncology
2006-2012
Universidad de Navarra
2010-2012
Jackson Laboratory
2007
Health Sciences Centre
2006
Transplantation of human acute myeloid leukemia (AML) and lymphoblastic (ALL) primary cells cell lines in different strains immunodeficient mice has led to preclinical models extensively used investigate stem cells, biology drug sensitivity. We studied the engraftment kinetics AML ALL 3 NOD.CB17-Prkdc(scid) (NOD/scid, NS)-related (NOD.Cg-Prkdc(scid)B2m(tm1Unc)/J, abbreviated NOD/scid/beta2 null, NSB; NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ, NOD/scid/IL-2Rgamma NSG). The malignant was...
Twenty-four patients with metastatic cancer received two cycles of four daily immunizations monocyte-derived dendritic cells (DC). DC were incubated preheated autologous tumor lysate and subsequently IFN-α, TNF-α, polyinosinic:polycytidylic acid to attain type 1 maturation. One dose was delivered intranodally, under ultrasound control, the rest intradermally in opposite thigh. Cyclophosphamide (day -7), GM-CSF (days 1-4), pegIFN alpha-2a 8) completed each treatment cycle. Pretreatment...
Semiconducting polymer nanoparticles (SPNs) are explored for applications in cancer theranostics because of their high absorption coefficients, photostability, and biocompatibility. However, SPNs susceptible to aggregation protein fouling physiological conditions, which can be detrimental vivo applications. Here, a method achieving colloidally stable low-fouling is described by grafting poly(ethylene glycol) (PEG) onto the backbone fluorescent semiconducting polymer,...
Methylimidoselenocarbamates have previously proven to display potent antitumor activities. In the present study we show that these compounds act as multikinase inhibitors. We found most effective compound, quinoline imidoselenocarbamate EI201, inhibits PI3K/AKT/mTOR pathway, which is persistently activated and contributes malignant progression in various cancers. EI201 blocked phosphorylation of AKT, mTOR several its downstream regulators (p70S6K 4E-BP1) ERK1/2 PC-3, HT-29 MCF-7 cells vitro,...
Dental pulp stem cells (DPSCs) from adult teeth express neural crest (NC) markers together with core transcriptional factors associated cell pluripotency, such as Oct4a, Sox2, c-Myc, Rex1, Stella/ Dppa3, Ssea1/Fut4, Lin28 and Nanog.The possibility to boost the natural stemness features of DPSCs by mild methods, that do not involve gene and/or chromatin modification or transfection, is highly desirable for therapy.Canonical Wnt Notch are two conserved developmental signalling pathways...
Blastic natural killer (NK) cell lymphoma/blastic plasmacytoid dendritic neoplasm (BNKL) is a rare and aggressive neoplasia characterized by infiltration of blast CD4(+)/CD56(+) cells in the skin, bone marrow, peripheral blood. Currently, more efforts are required to better define molecular biological mechanisms associated with this pathology. To best our knowledge, no mouse model recapitulated human BNKL so far.Primary marrow from patient were injected nonobese diabetes/severe combined...
The phosphoinositide 3-kinase (PI3K) pathway is believed to be of key importance in pediatric glioblastoma. Novel inhibitors the PI3K are being developed and entering clinical trials. Our aim identify potential non-invasive biomarkers signaling inhibition glioblastoma using vitro nuclear magnetic resonance (NMR) spectroscopy, aid identification target therapeutic response early phase trials childhood cancer. Treatment SF188 KNS42 human cell lines with dual pan-Class I PI3K/mTOR inhibitor...
Abstract Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression functional similarities with pluripotent cells. The stemness DPSCs also be pharmacologically enhanced by the activation canonical Wnt signaling. Here, we examined metabolic profile during reprogramming linked activation, short (48 hr) exposure either GSK3‐β inhibitor BIO (6‐bromoindirubin‐3´‐oxine) or human recombinant protein WNT‐3A. Both treatments...
Cell fusion plays a well-recognized physiological role during development, while its function progression is still unclear. Here, we show that acute myeloid leukemia (AML) cells spontaneously fused with murine host in vivo. AML most cases mouse macrophages. Other targets of cell were dendritic and endothelial cells. Cytogenetic molecular analysis revealed successive recipients conserved detectable amounts parental DNA. Moreover, AML1-ETO model where female AML1-ETO-leukemic cells, expressing...
// Alice Agliano 1 , Geetha Balarajah 1, 4 Daniela M. Ciobota Jasmin Sidhu Paul A. Clarke 2 Chris Jones 3 Workman Martin O. Leach and Nada M.S. Al-Saffar Cancer Research UK Imaging Centre, Division of Radiotherapy Imaging, The Institute Research, London, United Kingdom Therapeutics Unit, Divisions Molecular Pathology, Royal Marsden NHS Foundation Trust, Centre for Therapeutics, Correspondence to: Leach, email: Martin.Leach@icr.ac.uk Al-Saffar, Nada.Al-Saffar@icr.ac.uk Keywords: metabolic...
Recent experimental data showed that the PI3K pathway contributes to resistance temozolomide (TMZ) in paediatric glioblastoma and this effect is reversed by combination treatment of TMZ with a inhibitor. Our aim assess whether results metabolic changes are detectable nuclear magnetic resonance (NMR) spectroscopy, potentially providing biomarkers for inhibition treatment. Using two genetically distinct cell lines, SF188 KNS42, vitro 1H-NMR analysis following dual pan-Class I PI3K/mTOR...
Abstract Although radiotherapy is used to treat more than 50% of all cancer patients, in glioblastomas the percentage success limited due radioresistance glioma cells. Reports have shown that part this phenomenon has be attributed deregulation PI3K/mTOR pathway (AC Begg, Nat Rev, 2011). Furthermore, p110α isoform specific PI3K inhibitors produced additive cytotoxic effects combination with radiation therapy vitro and vivo glioblastoma models (Chen JS et al. MCT. 2008). Based on this, we...
<div>Abstract<p><b>Purpose:</b> Blastic natural killer (NK) cell lymphoma/blastic plasmacytoid dendritic neoplasm (BNKL) is a rare and aggressive neoplasia characterized by infiltration of blast CD4<sup>+</sup>/CD56<sup>+</sup> cells in the skin, bone marrow, peripheral blood. Currently, more efforts are required to better define molecular biological mechanisms associated with this pathology. To best our knowledge, no mouse model recapitulated...
<div>Abstract<p><b>Purpose:</b> Blastic natural killer (NK) cell lymphoma/blastic plasmacytoid dendritic neoplasm (BNKL) is a rare and aggressive neoplasia characterized by infiltration of blast CD4<sup>+</sup>/CD56<sup>+</sup> cells in the skin, bone marrow, peripheral blood. Currently, more efforts are required to better define molecular biological mechanisms associated with this pathology. To best our knowledge, no mouse model recapitulated...
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