A5009305853- Mesenchymal stem cell research
- dental development and anomalies
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Periodontal Regeneration and Treatments
- Graphene and Nanomaterials Applications
- Bone Tissue Engineering Materials
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- Dental materials and restorations
- RNA Research and Splicing
- Nerve injury and regeneration
- Cancer Cells and Metastasis
- Dental Trauma and Treatments
- Oral and Maxillofacial Pathology
University of the Basque Country
2016-2025
Tohoku University
2024
Université de Strasbourg
2006
Inserm
2006
Capital Medical University
2006
University of Tarapacá
2002
National Institutes of Health
2000
In tooth morphogenesis, the dental epithelium and mesenchyme interact reciprocally for growth differentiation to form proper number shapes of teeth. We previously identified epiprofin (Epfn), a gene preferentially expressed in epithelia, differentiated ameloblasts, certain ectodermal organs. To identify role Epfn development, we created Epfn-deficient mice (Epfn-/-). Epfn-/- developed an excess teeth, enamel deficiency, defects cusp root formation, abnormal dentin structure. Mutant germs...
Post-eruptive loss of ameloblasts requires identification alternative sources for these cells to realize tooth-tissue-engineering strategies. Recent reports showed that bone-marrow-derived can give rise different types epithelial cells, suggesting their potential serve as a source ameloblasts. To investigate this potential, we mixed c-Kit + -enriched bone marrow with embryonic dental and cultured them in re-association mesenchyme. Non-dividing, polarized, secretory ameloblast-like were...
Several stem cell sources persist in the adult human body, which opens doors to both allogeneic and autologous therapies. Tooth tissues have proven be a surprisingly rich accessible source of neural crest-derived ectomesenchymal cells (EMSCs), may employed repair disease-affected oral advanced regenerative dentistry. Additionally, one area medicine that demands intensive research on new is nervous system regeneration, since this constitutes therapeutic hope for patients affected by highly...
Background: We used an in vitro tooth development model to investigate the effects of overactivation Wnt/β-catenin pathway during odontogenesis by bromoindirubin oxime reagent (BIO), a specific inhibitor GSK-3 activity. Results: Overactivatingthe at initiation upregulated and ectopically expressed epithelial markers Sonic Hedgehog (Shh), Epiprofin (Epfn) Fibroblast growth factor8 (Fgf8), which are involved delimitation odontogenic fields oral ectoderm. This result indicated ectopic extension...
We identified a cDNA clone for epiprofin, which is preferentially expressed in teeth, by differential hybridization using DNA microarrays from an embryonic day 19.5 mouse molar library. Sequence analysis revealed that this encodes member of the Krüppel-like factor family containing three characteristic C2H2-type zinc finger motifs. The full-length was obtained 5′ Cap capture method. Except its 5′-terminal sequence, epiprofin mRNA sequence almost identical to predicted 14/Sp6 (specificity...
Phenotypic transformation of liver sinusoidal endothelial cells is one the most important stages metastasis progression. The miRNA effects on during have not yet been studied. Herein, whole genome analysis expression in these colorectal revealed repressed microRNA‐20a. Importantly, downregulation miR‐20a occurs parallel with upregulation its known protein targets. To restore normal levels cells, we developed chondroitin sulfate‐sorbitan ester nanoparticles conjugated a delivery system that...
Dental pulp stem cells (DPSCs) have the capacity to give rise with neuronal-like phenotypes, suggesting their use in brain cell therapies. In present work, we wanted address phenotypic fate of adult genetically unmodified human DPSCs cultured NeurocultTM (Stem Cell Technologies), a culture medium without serum which can be alternatively supplemented for expansion and/or differentiation neural (NSCs). Our results show that non-genetically modified Neurocult NS-A proliferation supplement...
The generation of vasculature is one the most important challenges in tissue engineering and regeneration. Human dental pulp stem cells (hDPSCs) are some promising cell types to induce vasculogenesis angiogenesis as they not only secrete vascular endothelial growth factor (VEGF) but can also differentiate vitro into both endotheliocytes pericytes serum-free culture media. Moreover, hDPSCs generate complete blood vessels containing mural layers vivo, upon transplantation adult brain. However,...
Dental pulp stem cells (DPSCs) from adult teeth show the expression of a very complete repertoire pluripotency core factors and high plasticity for cell reprogramming. Canonical Wnt Notch signaling pathways regulate stemness in DPSCs, even short-term (48 h) activations pathway induce profound remodeling DPSCs at physiologic metabolic levels. In this work, DPSC cultures were exposed to treatments modulating signaling, also induced differentiate osteo/adipocytes. DNA methylation, histone...
Human Dental Pulp Stem Cells (hDPSCs) are one of the most promising types cells to regenerate nerve tissues. Standard DMEM+10% fetal bovine serum (FBS) culture medium allows a fast expansion hDPSC as surface-adherent cell monolayer. However, use FBS also compromises clinical these protocols, and its longterm presence favors hDPSCs differentiation toward mesenchymal cell-derived lineages, at expense reduced capability generate neural cells. The objective this work was characterize role...
ABSTRACT The synchronization of cell proliferation and cytodifferentiation between dental epithelial mesenchymal cells is required for the morphogenesis teeth with correct functional shapes optimum sizes. Epiprofin (Epfn), a transcription factor belonging to Sp family, regulates essential ameloblast odontoblast differentiation. Epfn deficiency results in lack enamel ironically formation extra teeth. We investigated mechanism underlying functions tooth development through creation transgenic...
Dental pulp stem cells (DPSCs) from adult teeth express neural crest (NC) markers together with core transcriptional factors associated cell pluripotency, such as Oct4a, Sox2, c-Myc, Rex1, Stella/ Dppa3, Ssea1/Fut4, Lin28 and Nanog.The possibility to boost the natural stemness features of DPSCs by mild methods, that do not involve gene and/or chromatin modification or transfection, is highly desirable for therapy.Canonical Wnt Notch are two conserved developmental signalling pathways...
Human dental pulp stem cells (hDPSCs) constitute a promising alternative for central nervous system (CNS) cell therapy. Unlike other human cells, hDPSCs can be differentiated, without genetic modification, to neural that secrete neuroprotective factors. However, better understanding of their real capacity give rise functional neurons and integrate into synaptic networks is still needed. For that, ex vivo differentiation protocols must refined, especially avoid the use fetal animal serum. The...
Abstract Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression functional similarities with pluripotent cells. The stemness DPSCs also be pharmacologically enhanced by the activation canonical Wnt signaling. Here, we examined metabolic profile during reprogramming linked activation, short (48 hr) exposure either GSK3‐β inhibitor BIO (6‐bromoindirubin‐3´‐oxine) or human recombinant protein WNT‐3A. Both treatments...
Delta-like proteins 1 and 2 (DLK1, 2) are NOTCH receptor ligands containing epidermal growth factor-like repeats, which regulate signalling. We investigated the role of DLK pathway in morphogenesis submandibular salivary glands (SMGs), using vitro organotypic cultures.DLK1 were present all stages SMG morphogenesis, where DLK1 inhibited both activity branching. The addition inhibitory agents, either soluble (sDLK1) or N-[N-(3, 5-difluorophenacetyl-L-alanyl]-S-phenylglycine t-buthyl ester...
3D cell culture systems based on biological scaffold materials obtainable from both animal and human tissues constitute very interesting tools for therapy personalised medicine applications. The white adipose tissue (AT) extracellular matrix (ECM) is a promising biomaterial engineering due to its easy accessibility, malleability proven activity. In the present study, dental pulp stem cells (hDPSCs) were combined in vitro with ECM scaffolds porcine decellularised (pDAT, hDAT) processed as...
Hybrid hydrogels based on graphene derivatives and cerium oxide nanoparticles support heterocellular cultures for nervous tissue regeneration. Antioxidant properties of the allow survival both neurons oligodendrocytes.