Although acute renal failure is encountered with administration of nephrotoxic drugs, ischemia, or unilateral nephrectomy, there has been no effective drug which can be used in case failure. Hepatocyte growth factor (HGF) a potent hepatotropic for liver regeneration and known to have mitogenic, motogenic, morphogenic activities various epithelial cells, including tubular cells. Intravenous injection recombinant human HGF into mice remarkably suppressed increases blood urea nitrogen serum...

Tooth morphogenesis results from reciprocal interactions between oral epithelium and ectomesenchyme culminating in the formation of mineralized tissues, enamel, dentin. During this process, epithelial cells differentiate into enamel-secreting ameloblasts. Ameloblastin, an enamel matrix protein, is expressed by differentiating Here, we report creation ameloblastin-null mice, which developed severe hypoplasia. In mutant tooth, dental differentiated ameloblasts, but were detached subsequently...

Amorphous Mg–Cu–Y alloys with diameters (or thicknesses) ranging from 1 to 7 mm were formed in cylinder and sheet forms by using a high-pressure die casting process. The maximum diameter thickness) for formation of an amorphous phase was strongly dependent on composition the largest value obtained Mg65Cu25Y10. There is tendency increase supercooled liquid region. glass transition temperature, crystallization temperature heat are independent sample range below while relaxation decreases...

W e have demonstrated by affinity chromatography that hepatocyte growth factor (HGF) binds strongly to heparan sulfate (HS).This substantiates previous suggestions cell-surface proteoglycans constitute the so-called low cellular binding sites for HGF.Using a recombinant human HGF column, we analyzed effects of various specific chemical and enzymatic modificationddepolymerizations HS on its in order elucidate polysaccharide structural determinants.Interaction is shown be only slightly...

Chronic renal disease (CRD) is generally thought to be incurable, except through transplantation, and the number of patients with CRD on increase. Glomerulosclerosis tubulointerstitial fibrosis represent morphological equivalent end-stage CRD. In this study, we demonstrated preventive effect hepatocyte growth factor (HGF) progression dysfunction fibrosis, using a spontaneous mouse model for (ICGN strain). The mice progressively developed glomerular sclerotic injury, tubular atrophy, until...

Hepatocyte growth factor (HGF) has been shown to have hepatotrophic and renotropic functions for regeneration of the liver kidney through its mitogenic, motogenic, morphogenic properties. To examine involvement HGF in lung after acute injury, we analyzed changes mRNA, activity, receptors rat insult measured concentration sera patients with various diseases. Following onset injury induced by intratracheal hydrochloride injection, a compensatory DNA synthesis occurred bronchial epithelium peak...

In this study we asked whether members of thehedgehoggene family are involved in osteogenesis. C3H10T1/2 cells and MC3T3-E1 expressed the putative hedgehog receptorpatched(Ptc) gene. Medium conditioned by chicken embryo fibroblast cultures expressing either Indian or Sonic stimulated alkaline phosphatase (APase) activity mouse mesenchymal cell line osteoblastic MC3T3-E1. These stimulatory effects were synergistically enhanced bone morphogenetic protein-2 (BMP-2). Treatment with...

Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, underlying mechanisms still poorly understood. To address this issue, we assessed effect fibroblast-derived diffusible factors on growth morphogenetic properties a clonally derived subpopulation (clone TAC-2) normal murine gland (NMuMG) epithelial cells embedded collagen gels. Under control conditions, TAC-2 suspended within gels formed...

The pannexin proteins represent a new gap junction family. However, the cellular functions of pannexins remain largely unknown. Here, we demonstrate that 3 (Panx3) promotes differentiation osteoblasts and ex vivo growth metatarsals. Panx3 expression was induced during osteogenic C2C12 cells primary calvarial cells, suppression this endogenous inhibited differentiation. functioned as unique Ca2+ channel in endoplasmic reticulum (ER), which activated by purinergic receptor/phosphoinositide...

Hepatocyte growth factor (HGF), a most potent for mature hepatocytes may act as trigger liver regeneration. We reported that HGF strongly stimulates DNA synthesis of rabbit renal tubular cells in secondary culture (Igawa, T., Kanda, S., Kanetake, H., Saitoh, Y., Ichihara, A., Tomita, and Nakamura, T. (1991) Biochem. Biophys. Res. Commun. 174, 831-838). To investigate whether or not is involved regeneration, we examined changes mRNA, activity, receptor the rat kidney following unilateral...

The application of a high-pressure die casting technique to La–Al–TM (TM=Co, Ni, Cu) alloys was found produce bulk amorphous in cylinder and sheet forms. maximum sample diameter (dc) for formation an phase is 3 mm La55Al25(Ni or Cu)20, 7 La55Al25Ni10Cu10 above 9 La55Al25Cu10Ni5Co5. There clear tendency dc increase with the temperature interval supercooled liquid region. glass transition (Tg), crystallization (Tx) heat (ΔHx) remain unchanged thickness range 1 La55Al25Cu10Ni10 alloy. Tg ΔHx...

Hepatocyte growth factor (HGF), a potent mitogen for mature hepatocytes, possesses mitogenic and morphogenic activities renal epithelial cells. To examine the renotropic function of HGF, we investigated expression HGF mRNA activity in rat kidney after acute failure. When failure was induced by ischemia or HgCl2 administration, DNA synthesis occurred predominantly tubular cells located outer medulla with peak at 48 h treatments. In both injuries, increased markedly, reaching maximum 6 to 12...

Although the expression of hepatocyte growth factor (HGF) and its receptor, proto-oncogene c-met, has been demonstrated in central nervous system (CNS), function HGF CNS was not fully understood. In present studies, we determined effects on neuronal development neocortical explant mesencephalic neurons obtained from embryonic rat brain. clearly enhanced neurite outgrowth explants. culture, number tyrosine hydroxylase (TH)-positive significantly higher HGF-treated wells neurites TH-positive...

Hepatocyte growth factor (HGF), a mesenchymal-derived which regulates growth, motility, and morphogenesis of epithelial endothelial cells, functions as hepatotrophic renotrophic for regeneration the liver kidney. We have now obtained evidence that transforming factor-beta 1 (TGF-beta 1) glucocorticoids are negative regulators HGF gene expression. When TGF-beta or dexamethasone was added to cultures MRC-5 human embryonic lung fibroblasts HL-60 promyelocytic leukemic amount secreted into...

Pannexin 3 (Panx3) is a new member of the gap junction pannexin family, but its expression profiles and physiological function are not yet clear. We demonstrate in this study that Panx3 expressed cartilage regulates chondrocyte proliferation differentiation. mRNA was prehypertrophic zone developing growth plate induced during differentiation chondrogenic ATDC5 N1511 cells. Panx3-transfected cells promoted differentiation, suppression endogenous inhibited primary chondrocytes. reduced...