A5091657288- Schizophrenia research and treatment
- Parasitic Infections and Diagnostics
- Tryptophan and brain disorders
- Amoebic Infections and Treatments
- Treatment of Major Depression
- Bipolar Disorder and Treatment
- Functional Brain Connectivity Studies
- Reproductive Physiology in Livestock
- Toxoplasma gondii Research Studies
- Liver physiology and pathology
- Trypanosoma species research and implications
- Electroconvulsive Therapy Studies
- Smart Materials for Construction
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- RNA Interference and Gene Delivery
- Mental Health and Psychiatry
- Organ Transplantation Techniques and Outcomes
- Winter Sports Injuries and Performance
- Obsessive-Compulsive Spectrum Disorders
- Cryospheric studies and observations
- Immunotherapy and Immune Responses
- Stress Responses and Cortisol
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
Miwa Internal Medicine Clinic
2010-2020
Stanford University
1995-2019
University of Miami
2019
Palo Alto University
2019
University of California, San Diego
2019
Osaka University
2000-2018
Tokushima Breast Care Clinic
2009-2018
Tokushima University
1997-2013
Nihon University
2010-2012
University hospital Medical Information Network
2012
It is postulated that vascular disease involves a disturbance in the homeostatic balance of factors regulating tone and structure. Recent developments gene transfer techniques have emerged as an exciting therapeutic option to treat disease. Several studies established feasibility direct vivo into vasculature by using reporter genes such beta-galactosidase or luciferase. To date no study has documented effects with cDNA encoding functional enzyme. This tests hypothesis endothelium-derived...
Glomerulosclerosis, a final common lesion of various glomerular diseases, is characterized by mesangial cell proliferation and extracellular matrix (ECM) expansion. TGF-beta PDGF are known to play critical role in the regulation ECM metabolism mesenchymal proliferation, respectively. However, there little evidence demonstrate direct each these growth factors pathogenesis glomerulosclerosis. Using an vivo transfection technique, we could realize selective overexpression single factor kidney....
This preliminary study was performed to test the reliability and validity of Brief Assessment Cognition in Schizophrenia (BACS) as an assessment tool a Japanese-language version (BACS-J). The subjects for present were 30 outpatients with chronic schizophrenia. Each subject gave written informed consent participate research. Cronbach's alpha BACS-J 0.77. composite score significantly correlated all primary measures (verbal memory, working motor speed, verbal fluency, attention, executive...
Interstitial pneumonia is characterized by alveolitis with resulting fibrosis of the interstitium. To determine relevance humoral factors in pathogenesis interstitial pneumonia, we introduced expression vectors into Wistar rats via trachea to locally overexpress lungs. Human interleukin (IL) 6 and IL-6 receptor genes induced lymphocytic without marked fibroblast proliferation. In contrast, overexpression human transforming growth factor beta 1 or platelet-derived B gene only mild apparent...
Liposomes associated with gangliosides and loaded the human insulin gene were incubated HVJ (Sendai virus) red blood cell membranes containing non-histone chromosomal protein, high mobility group 1 (HMG-1), or bovine serum albumin (BSA) to form vesicle complexes. These complexes then injected into portal vein of adult rats. The co-introduced HMG-1 was transported nuclei liver cells much more efficiently than BSA. Moreover, amount transcript 10 times greater that Similar amounts DNA RNA found...
An RNA melanoma vaccine was investigated to induce protective immunity in a mouse-melanoma model. LacZ mRNA synthesized vitro by pSFV3 expression vector and introduced into the spleen of mice, using HVJ-liposomes. A high level beta-galactosidase activity detected for 10 days mouse spleen. The human melanoma-associated antigen gp100 encapsulated Immunization direct injection HVJ-liposomes induced both anti-gp100 Ab CTL responses against B16 melanoma. administration delayed tumor growth...
The plant alkaloid, berberine sulphate, inhibited the growth of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis in BI-S-33 medium, induced morphological changes parasites. Exposure E. histolytica to caused a clumping chromatin nucleus, formation autophagic vacuoles aggregates small cytoplasm. In berberine-treated G. lamblia, an irregularly-shaped vacuole appeared cytoplasm gradually enlarged during culture. trophozoites became swollen deposits glycogen were seen was also...
To determine if genetic diversity of Blastocystis hominis exists in Japan, we monitored 64 B. hominis-infected people: 39 asymptomatic people whose infections were detected during routine medical check-ups (32 Japanese and 7 non-Japanese) 25 patients with gastrointestinal symptoms who visited the outpatient clinics St. Luke's International Hospital (19 6 non-Japanese). We known 2 new riboprint patterns isolates from infected people. There no differences distribution ribodemes between...
Aim: In recent years, greater attention has been given to quality of life (QOL) in schizophrenia and several studies reported that negative depressive symptoms cognitive dysfunction are related patient QOL. But because a variety QOL measures have used the previous studies, there seems be no unanimous predictors for subjective objective The purpose present study was elucidate relationship between clinical variables outpatients with schizophrenia, using disease‐specific measures. Particular...
Objective. Numerous cytokines are expressed in lesions of synovial hyperplasia patients with rheumatoid arthritis (RA), and their pathophysiological contributions have been the subject speculation. These genes regulated by transcription factor NFκB which turn is activated tumour necrosis factor‐α (TNF‐α) cytokines. In this study we examined inhibition production pro‐inflammatory cytokines, adhesion molecule matrix metalloproteinase (MMP) from tissue RA introduction synthetic double‐stranded...
A lambda gt11 cDNA library was constructed from the poly(A)+ RNA of trophozoites Entamoeba histolytica HM-1:IMSS strain. The immunologically screened with monoclonal antibody 4G6, which is specific for 30,000-Mr antigen pathogenic isolates. 0.7-kb clone isolated, and its nucleotide sequence determined. To examine whether this gene isolates, a polymerase chain reaction performed by using four sets primers genomic DNA nonpathogenic isolates as templates. Amplified DNAs were detected not only...
A monoclonal antibody (MAb) produced against trophozoites of Entamoeba histolytica strain HM-1:IMSS, reacted with all 42 isolates and 4 clones showing pathogenic zymodeme (Z) patterns, i.e., Z-II, Z-II alpha-, (glucose phosphate isomerase: gamma +), Z-VII, Z-VII alpha lack, Z-XI, Z-XIV, Z-XIX, regardless culture conditions, geographical origins, or host symptoms in an indirect fluorescence test. In contrast, the MAb failed to react 14 possessing nonpathogenic zymodemes Z-I Z-VIII did not...
In addition to the atria, recent evidence suggests that atrial natriuretic peptide (ANP) is also synthesized in other tissues. Of particular interest location of ANP mRNA vessel wall. We and others have shown exogenously added inhibited growth endothelial cells vascular smooth muscle (VSMC) culture. However, it not known if locally would act similarly. Because cultured VSMC lost ability express endogenous gene, we transfected culture with an expression vector expressing rat examined effects...