A5024133387- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Cannabis and Cannabinoid Research
- Nicotinic Acetylcholine Receptors Study
- Multiple Sclerosis Research Studies
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Parkinson's Disease Mechanisms and Treatments
- S100 Proteins and Annexins
- Peripheral Neuropathies and Disorders
- Genetic Neurodegenerative Diseases
- Cerebrospinal fluid and hydrocephalus
- Neurogenesis and neuroplasticity mechanisms
- Ion Channels and Receptors
- Aluminum toxicity and tolerance in plants and animals
- Telomeres, Telomerase, and Senescence
- Nuclear Receptors and Signaling
- Genetics, Aging, and Longevity in Model Organisms
- Sleep and Wakefulness Research
- Trace Elements in Health
- COVID-19 Clinical Research Studies
Achucarro Basque Center for Neuroscience
2022-2025
University of the Basque Country
2022-2025
BioCruces Health research Institute
2022-2025
University of Minnesota
2020-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2022-2024
University of Minnesota System
2024
Instituto Cajal
2023
Universidad Autónoma de Madrid
2015-2018
Hospital Universitario de La Princesa
2015-2018
University of Castilla-La Mancha
2018
Abstract Alzheimer's disease (AD) is associated with senile plaques of beta‐amyloid (Aβ) that affect the function neurons and astrocytes. Brain activity results from coordinated astrocytes in astroglial‐neuronal networks. However, effects Aβ on astroglial neuronal network remains unknown. Simultaneously monitoring astrocyte calcium electric activities, we quantified impact sensory‐evoked cortical a mouse model AD. At rest, displayed spontaneous hyperactivity was related to density....
Cortical pathology involving inflammatory and neurodegenerative mechanisms is a hallmark of multiple sclerosis correlate disease progression cognitive decline. Astrocytes play pivotal role in initiation but astrocyte-neuronal network alterations contributing to gray matter remain undefined. Here we unveil deregulation astrocytic calcium signaling astrocyte-to-neuron communication as key pathophysiological cortical dysfunction the experimental autoimmune encephalomyelitis (EAE) model...
Human dental pulp stem cells (hDPSCs) constitute a promising alternative for central nervous system (CNS) cell therapy. Unlike other human cells, hDPSCs can be differentiated, without genetic modification, to neural that secrete neuroprotective factors. However, better understanding of their real capacity give rise functional neurons and integrate into synaptic networks is still needed. For that, ex vivo differentiation protocols must refined, especially avoid the use fetal animal serum. The...
ABSTRACT Cortical damage and dysfunction is a pathological hallmark of multiple sclerosis (MS) that correlates with the severity physical cognitive disability. Astrocytes participate in MS pathobiology through variety mechanisms, abnormal astrocytic calcium signaling has been pointed as pathogenic mechanism cortical MS. However, vivo evidence supporting deregulation astrocyte calcium‐dependent mechanisms still limited. Here, we applied fiber photometry to longitudinal analysis spontaneous...
Rapid increases in the aging population present a critical challenge to global societies, with major concern being cognitive decline and brain aging. While neuroscientific research has traditionally focused on neurons, role of astrocytes is recent interest but remains underexplored. Astrocytes, homeostatic cells central nervous system, play essential roles synaptic regulation, neurotransmitter clearance, neuronal support, homeostasis. Recent studies indicate that undergo significant...
Navigation and memory functions are essential for survival regulated by the hippocampus. These processes tightly controlled, one of key modulators involved is endocannabinoid system, particularly through cannabinoid receptor type-1 (CB1). CB1 widely expressed in various hippocampal cell types. While it known that participates processes, its specific roles different types how these may differ between sexes remain unclear. This study investigates signaling hippocampus, both, cell-type-specific...
Microcircuits in the neocortex are functionally organized along layers and columns, which fundamental modules of cortical information processing. While function microcircuits has focused on neuronal elements, much less is known about functional organization astrocytes their bidirectional interaction with neurons. Here, we show that Cannabinoid type 1 receptor (CB1R)-mediated astrocyte activation by neuron-released endocannabinoids elevate Ca2+ levels, stimulate ATP/adenosine release as...
Astrocytes are active cells involved in brain function through the bidirectional communication with neurons, which astrocyte calcium plays a crucial role. Synaptically evoked increases can be localized to independent subcellular domains or expand entire cell, i.e., surge. Because single may contact ~100,000 synapses, control of intracellular signal propagation have relevant consequences on function. Yet, properties governing spatial dynamics remains poorly defined. Imaging responses cortical...
Astrocytes are active cells involved in brain function through the bidirectional communication with neurons, which astrocyte calcium signal plays a crucial role. Synaptically-evoked increases can be localized to independent subcellular domains or expand entire cell, i.e., surge. In turn, astrocytes may regulate individual synapses by calcium-dependent release of gliotransmitters. Because single contact ∼100,000 synapses, control intracellular propagation have relevant consequences on...
We characterized the ionic currents underlying cellular excitability and Ca(2+) -channel subtypes involved in action potential (AP) firing of rat adrenal chromaffin cells (RCCs) preserved their natural environment, gland slices, through perforated patch-clamp recording technique. RCCs prepared from slices exhibit a resting -54 mV, spontaneous APs (2-3 spikes/s) generated by opening Na(+) -channels, terminated activation voltage -activated K(+) -channels (BK). influx via L-type is reaching...
As the peripheral sympathoadrenal axis is tightly controlled by cortex via hypothalamus and brain stem, central pathological features of Hunting's disease, (HD) that is, deposition mutated huntingtin synaptic dysfunctions, could also be expressed in adrenal chromaffin cells. To test this hypothesis we here present a thorough investigation on functional changes undergone cells (CCs) from 2-month (2 m) to 7-month (7 aged wild-type (WT) R6/1 mouse model Huntington's disease (HD), stimulated...
It is currently known that in CNS the extracellular matrix involved synaptic stabilization and limitation of plasticity. However, it has been reported treatment with chondroitinase following injury allows formation new synapses increased plasticity functional recovery. So, we hypothesize some components may modulate transmission. To test this hypothesis evaluated effects chondroitin sulphate (CS) on excitatory transmission, cellular excitability, neuronal using recordings CA1 area rat...
Astrocytes are active cells involved in brain function through the bidirectional communication with neurons, which astrocyte calcium plays a crucial role. Synaptically evoked increases can be localized to independent subcellular domains or expand entire cell, i.e., surge. Because single may contact ~100,000 synapses, control of intracellular signal propagation have relevant consequences on function. Yet, properties governing spatial dynamics remains poorly defined. Imaging responses cortical...
Abstract Antisense oligonucleotide (ASO) therapy for neurological disease has been successful in clinical settings and its potential generated hope Alzheimer’s (AD). We previously described that ablating SNCA encoding α-synuclein (αSyn) a mouse model of AD was beneficial. Here, we sought to demonstrate whether transient reduction αSyn expression using ASO could be therapeutic AD. The efficacy the measured via immunocytochemistry, RT-qPCR western blotting. To assess spatial learning memory,...
The inhibition of nicotinic acetylcholine receptors (nAChRs) has been proposed as a potential strategy to develop new antidepressant drugs. This is based on the observation that antidepressants selectively block noradrenaline (NA) or serotonin (5-HT) reuptake also inhibit nAChRs. Dual blocking both NA and 5-HT were shorten delay in exerting their clinical effects; whether duloxetine, prototype dual antidepressants, blocks nAChRs unknown. Here we explored this question bovine chromaffin cells...
Astrocytes are active cells involved in brain function through the bidirectional communication with neurons, which astrocyte calcium signal plays a crucial role. Synaptically-evoked increases can be localized to independent subcellular domains or expand entire cell, i.e., surge. In turn, astrocytes may regulate individual synapses by calcium-dependent release of gliotransmitters. Because single contact ∼100,000 synapses, control intracellular propagation have relevant consequences on...