A5007502698- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Treatment and Pharmacology
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Cancer, Lipids, and Metabolism
- Cancer Genomics and Diagnostics
- Chemokine receptors and signaling
- Body Contouring and Surgery
- RNA modifications and cancer
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Breast Cancer Treatment Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Vascular Tumors and Angiosarcomas
- Immune Cell Function and Interaction
- Metabolism, Diabetes, and Cancer
- Cancer Research and Treatments
- Glioma Diagnosis and Treatment
European Institute of Oncology
2015-2024
Ripamonti
2002-2024
Politecnico di Milano
2022-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2024
The University of Texas MD Anderson Cancer Center
2014
Fondazione IRCCS Istituto Neurologico Carlo Besta
2012
Pfizer (United States)
2011
Universität Hamburg
2011
University Medical Center Hamburg-Eppendorf
2011
University of California, Davis
2011
Metronomic chemotherapy has shown efficacy in patients with metastatic breast cancer. When used association targeted antiangiogenic drugs, it was more active than metronomic therapy alone preclinical and clinical studies.Patients advanced cancer were candidates to receive oral capecitabine (500 mg thrice daily) cyclophosphamide (50 plus bevacizumab (10 mg/kg every 2 weeks).In 46 assessable patients, we observed one complete response (CR; 2%), 21 partial responses (PR; 46%), 19 (41%) stable...
Transplantation of human acute myeloid leukemia (AML) and lymphoblastic (ALL) primary cells cell lines in different strains immunodeficient mice has led to preclinical models extensively used investigate stem cells, biology drug sensitivity. We studied the engraftment kinetics AML ALL 3 NOD.CB17-Prkdc(scid) (NOD/scid, NS)-related (NOD.Cg-Prkdc(scid)B2m(tm1Unc)/J, abbreviated NOD/scid/beta2 null, NSB; NOD.Cg-Prkdc(scid)Il2rg(tm1Wjll)/SzJ, NOD/scid/IL-2Rgamma NSG). The malignant was...
Abstract Purpose: Antigenic overlap among circulating endothelial cells (CEC) and progenitors (CEP), platelets, other blood led to the need develop a reliable standardized method for CEC CEP quantification. These are emerging as promising preclinical/clinical tools define optimal biological doses of antiangiogenic therapies help stratify patients in clinical trials. Experimental Design: We report experimental validation novel flow cytometry that precisely dissects CEC/CEP from platelets cell...
Abstract Previous studies have suggested a “catalytic role” in neoplastic angiogenesis and cancer progression for bone marrow–derived endothelial progenitor cells (EPC). However, preclinical clinical shown that the quantitative role of marrow-derived EPCs vascularization is extremely variable. We found human murine white adipose tissue (WAT) very rich reservoir CD45-CD34+ with differentiation potential, containing mean 263 times more cells/mL than marrow. Compared CD34+ mobilized blood by...
The human white adipose tissue (WAT) contains progenitors with cooperative roles in breast cancer (BC) angiogenesis, local and metastatic progression. biguanide Metformin (Met), commonly used for Type 2 diabetes, might have activity against BC was found to inhibit angiogenesis vivo . We studied Met another biguanide, phenformin (Phe), vitro models. In , biguanides activated AMPK, inhibited Complex 1 of the respiratory chain induced apoptosis WAT endothelial cells. coculture, production...
Genetic and molecular studies suggest that activin receptor-like kinase 1 (ALK1) plays an important role in vascular development, remodeling, pathologic angiogenesis. Here we investigated the of ALK1 angiogenesis context common proangiogenic factors [PAF; VEGF-A basic fibroblast growth factor (bFGF)]. We observed PAFs stimulated ALK1-mediated signaling, including Smad1/5/8 phosphorylation, nuclear translocation Id-1 expression, cell spreading, tubulogenesis endothelial cells (EC). An...
Anti-PD-1 and anti-PD-L1 checkpoint inhibitors (CIs) are clinically active in many types of cancer. However, only a minority patients achieve complete and/or long-lasting clinical response. We studied the effects different doses three widely used, orally chemotherapeutics (vinorelbine, cyclophosphamide 5-FU) over local metastatic tumour growth, landscape circulating tumour-infiltrating immune cells involved CI activity.Immunocompetent Balb/c mice were used to generate models breast cancer...
PURPOSE: The association of chemotherapy and antiangiogenic drugs has shown efficacy in clinical oncology. However, there is a need for biomarkers that allow selection patients who are likely to benefit from such treatment useful indicating best drug combination schedule. EXPERIMENTAL DESIGN: We investigated the predictive potential six angiogenic molecules/transcripts nine subpopulations circulating endothelial cells (CEC) progenitors (CEP) 46 with advanced breast cancer treated metronomic...
Obesity is associated with an increased frequency, morbidity, and mortality of several types neoplastic diseases, including postmenopausal breast cancer. We found that human adipose tissue contains two populations progenitors cooperative roles in CD45(-)CD34(+)CD31(+)CD13(-)CCRL2(+) endothelial cells can generate mature capillaries. Their cancer-promoting effect the was limited absence CD45(-)CD34(+)CD31(-)CD13(+)CD140b(+) mesenchymal progenitors/adipose stromal (ASC), which generated...
A cell population with progenitor-like phenotype (CD45-CD34+) resident in human white adipose tissue (WAT) is known to promote the progression of local and metastatic breast cancer angiogenesis. However, molecular mechanisms interaction have not been elucidated. In this study, we identified two proteins that were significantly upregulated WAT-derived progenitors after coculture cancer: granulocyte macrophage colony-stimulating factor (GM-CSF) matrix metallopeptidase 9 (MMP9). These released...
Abstract Metformin can induce breast cancer (BC) cell apoptosis and reduce BC local metastatic growth in preclinical models. Since is frequently used along with Aspirin or beta-blockers, we investigated the effect of Metformin, beta-blocker Atenolol several In vitro , synergized inducing triple negative endocrine-sensitive cells activating AMPK white adipose tissue (WAT) progenitors known to cooperate progression. Both added inhibitory against complex I respiratory chain. both...
Abstract Checkpoint inhibitors (CI) instigate anticancer immunity in many neoplastic diseases, albeit only a fraction of patients. The clinical success cyclophosphamide (C)-based haploidentical stem-cell transplants indicates that this drug may re-orchestrate the immune system. Using models triple-negative breast cancer (TNBC) with different intratumoral contexture, we demonstrate combinatorial therapy intermittent C, CI, and vinorelbine activates antigen-presenting cells (APC), abrogates...