A5007784597- Mechanisms of cancer metastasis
- Ferrocene Chemistry and Applications
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- Metalloenzymes and iron-sulfur proteins
- Enzyme Structure and Function
- Phosphodiesterase function and regulation
- Biosensors and Analytical Detection
- Porphyrin Metabolism and Disorders
- Cancer Cells and Metastasis
- Autoimmune Bullous Skin Diseases
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Eosinophilic Disorders and Syndromes
- RNA regulation and disease
- Contact Dermatitis and Allergies
- Metabolism, Diabetes, and Cancer
- T-cell and B-cell Immunology
- Bone Tissue Engineering Materials
- Cancer Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Advanced Proteomics Techniques and Applications
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Folate and B Vitamins Research
Misawa City Hospital
2024
National Institutes of Health
1981-2021
National Institute of Dental and Craniofacial Research
2019-2021
Henry Ford Hospital
2014
Science Applications International Corporation (United States)
2012-2013
National Cancer Institute
2008-2013
Center for Cancer Research
2010-2013
Frederick National Laboratory for Cancer Research
2013
Translational Research Institute
2012
University School
2012
BackgroundPrevious studies identified the human nonmetastatic gene 23 (NME1, hereafter Nm23-H1) as first metastasis suppressor gene. An inverse relationship between Nm23-H1 and expression of lysophosphatidic acid receptor 1 (LPAR1, also known EDG2 or LPA1) has been reported. However, effects LPA1 inhibition on primary tumor size, metastasis, metastatic dormancy have not investigated.
Abstract Nm23-H1 has been identified as a metastasis suppressor gene, but its protein interactions have yet to be understood with any mechanistic clarity. In this study, we evaluated the proteomic spectrum of made by in 4T1 murine breast cancer cells derived from tissue culture, primary mammary tumors, and pulmonary metastases. By approach, actin-severing Gelsolin binding partner for Nm23-H1, verifying their interaction coimmunoprecipitation well human MCF7, MDA-MB-231T, MDA-MB-435 cells....
Pancreatic adenocarcinoma has an incidence rate nearly equal to the mortality and is fourth leading cause of cancer-related death in USA. This largely due late symptom onset diagnosis. Evidence emerged that new-onset diabetes may be a caused by occult pancreatic cancer. We report case middle-aged African American female who presented with hyperglycemia persistent scapular tenderness. She was subsequently diagnosed metastatic cancer confirmed liver biopsy. did not have or pre-diabetes 6...
ABSTRACT The vast majority of mammalian genomes are transcribed as non-coding RNA in what is referred to “pervasive transcription.” Recent studies have uncovered various families upstream transcription start sites. In particular, highly unstable promoter transcripts known PROMPTs been shown be targeted for exosomal degradation by the nuclear exosome targeting complex (NEXT) consisting helicase MTR4, zinc-knuckle scaffold ZCCHC8, and binding protein RBM7. Here, we report that addition its...
Abstract The control of micrometastatic cancer is vital to clinical success in the adjuvant or post-treatment (minimal residual disease) settings. Over twenty metastasis suppressor genes (MSGs) have been validated; when over-expressed, they exert no significant effect on primary tumor growth but significantly reduce metastasis. Translation this basic research clinic difficult, as it virtually impossible re-express a gene every cell. An alternate approach identify whose expression inversely...
Abstract Whether screening small mammal serum during antibody production or attempting to preserve a stock of precious antibody, this protocol's western blotting method using aliquots containing nanoliter volumes will benefit researchers. Time‐tested workflows allowing separation and analysis proteins are routinely utilized in clinical laboratory settings. The necessity for relatively large quantities is major limitation universal tool. This article provides step‐by‐step protocol detecting...
Abstract In recent decades, a distinction has been made between the tumorigenesis and metastasis processes. Metastasis Suppressor Genes (MSGs) are down-regulated in compared with primary tumor. When re-expressed experimental models, MSGs able to block spread distant sites without effecting tumor formation. After discovery of first MSG, NME1, many others have identified. To date more than 20 genes described MSG functions. They include factors involved cellular proliferation, motility,...
Abstract We hypothesized that Nm23-H1 may suppress metastasis through binding and inactivation of proteins promote aggressive behavior. An unbiased survey was conducted in a 4T1 murine mammary carcinoma model system. Flag tagged its mouse variant, -M1, were transfected into cancer cells. Clonal lines inoculated the number four fat pad mice allowed to grow for 10 days prior excision due size primary tumor. Mice then imaged weekly remainder experiment, 8 weeks follow metastatic spread cells...
Abstract Metastasis Suppressor Genes (MSGs) are able to inhibit metastasis without reducing primary tumor size. Nm23 was the first MSGs be identified. The biochemical basis for anti-metastatic effect of has been subject many studies but remains unresolved. We performed co-immunoprecipitation assays using flag-tagged constructs both human and murine forms (Nm23-H1 Nm23-M1, respectively) over-expressed in mouse mammary carcinoma cell line 4T1. Co-immunoprecipitation were also on tissue...
<p>PDF file, 81K, Nm23-H1 interacts with Gelsolin in overexpressing MDA-MB-435 and HEK293TN cells.</p>
<p>PDF file, 180K, Latrunculin B treatment distrupts F-actin cytoskeleton structure.</p>
<p>PDF file, 180K, Latrunculin B treatment distrupts F-actin cytoskeleton structure.</p>
<div>Abstract<p><i>Nm23-H1</i> has been identified as a metastasis suppressor gene, but its protein interactions have yet to be understood with any mechanistic clarity. In this study, we evaluated the proteomic spectrum of made by Nm23-H1 in 4T1 murine breast cancer cells derived from tissue culture, primary mammary tumors, and pulmonary metastases. By approach, actin-severing Gelsolin binding partner for Nm23-H1, verifying their interaction coimmunoprecipitation well...
<p>PDF file, 86K.</p>
<p>PDF file, 81K, Nm23-H1 interacts with Gelsolin in overexpressing MDA-MB-435 and HEK293TN cells.</p>
<p>PDF file, 109K, Gelsolin and Nm23-H1 overexpression in 4T1 MDA-MB-231T cells is not associated with apoptosis.</p>