A5053287301- Zebrafish Biomedical Research Applications
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Mechanisms of cancer metastasis
- Science, Research, and Medicine
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Environmental Science and Water Management
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- HER2/EGFR in Cancer Research
- Angiogenesis and VEGF in Cancer
- Aldose Reductase and Taurine
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Pancreatitis Pathology and Treatment
- Medical Imaging and Pathology Studies
- Acute Lymphoblastic Leukemia research
- ATP Synthase and ATPases Research
- Immunodeficiency and Autoimmune Disorders
- Lung Cancer Research Studies
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
Kanazawa University
2024
National University of Singapore
2007-2023
National Institute of Technology, Tsuruoka College
2018-2023
National University Cancer Institute, Singapore
2021
Chiba Cancer Center
2021
Nippon Shokubai (Japan)
2020
Waseda University
2019
Kagawa University
2019
Tohoku University
2019
Ehime University
2019
BackgroundPrevious studies identified the human nonmetastatic gene 23 (NME1, hereafter Nm23-H1) as first metastasis suppressor gene. An inverse relationship between Nm23-H1 and expression of lysophosphatidic acid receptor 1 (LPAR1, also known EDG2 or LPA1) has been reported. However, effects LPA1 inhibition on primary tumor size, metastasis, metastatic dormancy have not investigated.
Metastasis of cancer cells is multi-step process and dissemination an initial step. Here we report a tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line as new animal model for metastasis research, demonstrate that this can serve novel platform discovery antimetastasis drugs targeting metastatic cells. By crossing with xmrk (a homolog hyperactive form EGFR) zebrafish, which develops hepatocellular carcinoma, approximately 80% the double showed spontaneous cell mCherry-labeled...
Abstract While loss of function (LOF) retinoblastoma 1 (RB1) tumor suppressor is known to drive initiation small‐cell lung cancer and retinoblastoma, RB1 mutation rarely observed in breast cancers at their initiation. In this study, we investigated the impact on untransformed mammary epithelial cells given by LOF. Depletion anon‐tumorigenic MCF10A induced reversible growth arrest (quiescence) featured downregulation multiple cyclins MYC, upregulation p27 KIP1 , lack expression markers which...
Abstract RECK has been described to modulate extracellular matrix components through negative regulation of MMP activities. Recently, was demonstrated bind an orphan G protein‐coupled receptor GPR124 mediate WNT7 signaling in nontumor contexts. Here, we attempted clarify the role driving WNT cancer cells. and formed a complex 293T cells, when both were expressed, significantly enhanced WNT7‐dependent manner. This cooperation abolished mutants unable transduced. stimulated growth KRAS‐mutated...
Antifolates are a class of drugs effective for treating malignant pleural mesothelioma (MPM). The majority antifolates inhibit enzymes involved in purine and pyrimidine synthesis such as dihydrofolate reductase (DHFR), thymidylate synthase (TYMS), glycinamide ribonucleotide formyltransferase (GART). In order to select the most suitable patients therapy with targeting specific metabolic pathways, there is need better predictive biomarkers. can alter global pathways MPM cells, yet profile...
Metastasis is responsible for approximately 90% of cancer-associated mortality but few models exist that allow rapid and effective screening anti-metastasis drugs. Current mouse metastasis are too expensive time consuming to use high-throughput screening. Therefore, we created a unique concept utilizing conserved mechanisms between zebrafish gastrulation cancer identification potential anti-metastatic We hypothesized small chemicals interrupt might also suppress metastatic progression cells...
Deoxyribonucleotide biosynthesis from ribonucleotides supports the growth of active cancer cells by producing building blocks for DNA. Although ribonucleotide reductase (RNR) is known to catalyze rate-limiting step de novo deoxyribonucleotide triphosphate (dNTP) synthesis, biological function RNR large subunit (RRM1) in small-cell lung carcinoma (SCLC) remains unclear. In this study, we established siRNA-transfected SCLC cell lines investigate anticancer effect silencing RRM1 gene...
Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic line was established to serve as platform identification. By crossing with xmrk (a homolog hyperactive form epidermal growth factor receptor) zebrafish, which develop hepatocellular carcinoma, approximately 80% double show spontaneous cell dissemination mCherry-labeled hepatocytes from liver entire abdomen and...
Few models exist that allow for rapid and effective screening of anti-metastasis drugs. Here, we present a drug protocol utilizing gastrulation zebrafish embryos identification Based on the evidence metastasis proceeds through molecular mechanisms gastrulation, hypothesized chemicals interrupting might suppress cancer cells. Thus, developed phenotype-based chemical screen uses epiboly, first morphogenetic movement in as marker. The only needs enables hundreds to be tested five hours by...
Abstract Metastasis is responsible for approximately 90% of cancer-associated mortality but few models exist that allow rapid and effective screening anti-metastasis drugs. Current mouse metastasis are too expensive time consuming to use high-throughput screening. Therefore, we created a unique concept utilizing conserved mechanisms between zebrafish gastrulation cancer identification potential anti-metastatic We hypothesized small chemicals interrupt might also suppress metastatic...
Abstract Metastasis, a leading contributor to the morbidity of cancer patients, occurs through multiple steps. As each these steps is promoted by different molecular mechanisms, blocking metastasis needs target Here we report that cinnamon bark extract (CBE) has suppressor effect on metastatic dissemination cells. Though zebrafish embryo screen which utilizes conserved mechanisms between and gastrulation for identifying anti-metastasis drugs, CBE was identified interfere with progression...
Abstract Abnormal biosyntheses of steroid hormones and dysregulation hormone receptors contribute to breast cancer metastasis but the mechanisms that are poorly understand. Here we report a stress producing enzyme, Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11β1) promotes metastasis. HSD11β1 was ectopically expressed in seventy-one percent triple-negative tumors correlated with shorter overall survival. significantly promoted through induction epithelial-to-mesenchymal transition (EMT);...
Abstract Loss of expression Nm23 significantly contributes to metastasis progression without effect on primary tumor growth. This is a hallmark suppressor genes (MSGs). Translation MSG basic research the clinic difficult since it impossible re-express gene in every cell. Previous studies identified G-protein coupled receptor for bioactive lipid lysophophatidic acid (LPA1) as being inversely correlated with Nm23-H1. LPA1 inhibitor (Debio 0719) treatment decreased two model systems breast...
<p>Figures S1. Liver-specific expression of Twist1a-ERT2 mRNA and increase mesenchymal marker Vimentin in liver cells transgenic zebrafish. Figure S2. Lack significant effect Adrenosterone, Rabeprazole Olmesartan primary tumor growth, fish survival, cell proliferation apoptosis Twist1a-ERT2/xmrk double S3. Effect Adrenosterone or on viabilities highly metastatic human lines, related to Fig. 4. S4. Expression markers appearance E -cad+ after adrenosteroneadrenosterone treatment HCCLM3....