A5061842530- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Vitamin D Research Studies
- Glycosylation and Glycoproteins Research
- Genetic factors in colorectal cancer
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Adrenal Hormones and Disorders
- HIV-related health complications and treatments
- Hormonal Regulation and Hypertension
- Psoriasis: Treatment and Pathogenesis
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Bone health and osteoporosis research
- Sexual Differentiation and Disorders
- HIV/AIDS drug development and treatment
- Metabolism and Genetic Disorders
- Bone health and treatments
- Cancer-related molecular mechanisms research
- HIV Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
23andMe (United States)
2023-2024
Children's Health Ireland at Crumlin
2021-2024
University College Dublin
2010-2024
University of Minnesota Rochester
2023
Stanford University
2009-2022
Mater Misericordiae University Hospital
2012-2022
St. Vincent's University Hospital
2003-2020
Baxter (United States)
2009-2018
University College London
2014-2017
University of Zimbabwe
2017
Pancreatic ductal adenocarcinoma (PDAC) is a genomically diverse, prevalent, and almost invariably fatal malignancy. Although conventional genetically engineered mouse models of human PDAC have been instrumental in understanding pancreatic cancer development, these are much too labor-intensive, expensive, slow to perform the extensive molecular analyses needed adequately understand this disease. Here we demonstrate that retrograde injection either adenoviral-Cre or lentiviral-Cre vectors...
C3a and C5a anaphylatoxins are cytokine-like polypeptides generated during complement (C) system activation released at the inflammatory site. They exert several biological activities through binding to G-protein-coupled receptors C3aR C5aR, respectively. Cloning Northern blot experiments have indicated that both expressed by myeloid as well nonmyeloid cells (e.g., endothelial epithelial cells). To better understand roles of C inflammation, we investigated their effects on expression...
The extent to which early events shape tumor evolution is largely uncharacterized, even though a better understanding of these may help identify key vulnerabilities in advanced tumors. Here, using genetically defined mouse models small cell lung cancer (SCLC), we uncovered distinct metastatic programs attributable the type origin. In one model, tumors gain ability through amplification transcription factor NFIB and widespread increase chromatin accessibility, whereas other become absence...
There is a need for new molecular-guided contrast agents to enhance surgical procedures such as tumor resection that require high degree of precision. Cysteine cathepsins are highly up-regulated in wide variety cancers, both cells and the tumor-supporting surrounding stroma. Therefore, tools can be used dynamically monitor their activity vivo could imaging intraoperative fluorescence image guided surgery (FGS). Although multiple classes cathepsin-targeted substrate probes have been reported,...
Telomere extension has been proposed as a means to improve cell culture and tissue engineering treat disease. However, telomere by nonviral, nonintegrating methods remains inefficient. Here we report that delivery of modified mRNA encoding TERT human fibroblasts myoblasts increases telomerase activity transiently (24-48 h) rapidly extends telomeres, after which telomeres resume shortening. Three successive transfections over 4 d period extended up 0.9 kb in type-specific manner conferred an...
The kinase LKB1 is a critical tumor suppressor in sporadic and familial human cancers, yet the mechanisms by which it suppresses growth remain poorly understood. To investigate tumor-suppressive capacity of four canonical families substrates vivo, we used CRISPR/Cas9-mediated combinatorial genome editing mouse model oncogenic KRAS-driven lung adenocarcinoma. We demonstrate that members SIK family are for constraining development. Histologic gene-expression similarities between LKB1-...
Background: Sun exposure of the skin, independent dietary sources, may provide sufficient vitamin D in healthy individuals. A recent study patients with cutaneous lupus erythematosus concluded that over 70% them restrict their sun exposure. Methods: We recruited 52 biopsy‐proven to establish whether they are deficient 25‐hydroxyvitamin [25(OH)D]. measured serum 25(OH)D levels during summer months, investigated effects several variables on and assessed role supplementation. Results: An...
Varicella-zoster virus (VZV) is a human alphaherpesvirus that highly cell associated in culture. Because cell-free yields are too low to permit the synchronous infections needed for time-resolved analyses, information lacking about sequence of events during VZV replication cycle. To address this challenge, we differentially labeled VZV-infected inoculum cells (input) and uninfected (output) with fluorescent dyes or endocytosed nanogold particles evaluated newly infected by confocal...
Little is known about vitamin D status in preterm infants and their response to supplementation. To investigate this, we assessed serum 25-hydroxyvitamin (25OHD) levels using RIA a consecutive sample of stable very low birth weight (VLBW) (born ≤ 32 weeks gestation or 1·5 kg), explored associated factors. Serum 25OHD level was first once were tolerating feeds ( n 274). If this below 50 nmol/l (20 ng/ml), which the with covering requirements terms skeletal health majority, then recommended...
The relationship between prevalence of multiple sclerosis (MS) and latitude may be due to both genetic environmental factors. hypothesis that, in Ireland, MS is increasing that north-south differences relate variation serum 25-hydroxyvitamin D (25(OH)D) levels was tested this study.Patients matched control subjects were identified counties Donegal, Wexford South Dublin through sources. Prevalence determined. Blood samples taken for 25(OH)D intact parathyroid hormone measurement, DNA...
Low bone mineral density (BMD) is common in HIV-positive patients, although the role played by HIV infection versus sociodemographic and metabolic factors remains unclear.Understanding Pathology of Bone Disease HIV-infected individuals (HIV UPBEAT) a prospective cohort study, enrolled HIV-negative participants from similar demographic backgrounds. Dual X-ray absorptiometry at femoral neck, total hip lumbar spine blood tests were performed. Associations between BMD interest assessed using...
The activation of an immune response in tomato (Solanum lycopersicum) against Pseudomonas syringae relies on the recognition E3 ligase-deficient forms AvrPtoB by host protein kinase, Fen. To investigate mechanisms which Fen-mediated immunity is regulated, we characterize this study a Fen-interacting protein, Fni3, and its cofactor, S. lycoperiscum Uev (Suv). Fni3 encodes homolog Ubc13-type ubiquitin-conjugating enzyme that catalyzes exclusively Lys-63-linked ubiquitination, whereas Suv...
Direct-to-consumer testing (DTCT) refers to commercial laboratory tests initiated by laypersons without the involvement of healthcare professionals. As this market grows in size and variety products, a clear definition DTCT ground conceptualization their harms benefits is needed. We describe how three different modalities (home self-testing, self-sampled tests, direct access tests) present caveats traditional process ('brain-to-brain loop'), might differ between medical vs. non-medical...
Endosialin (tumor endothelial marker 1) is expressed preferentially by tumor cells but not normal endothelium. Its protein domain architecture homologous to that of CD93 and thrombomodulin (CD141), suggesting a similar function in mediating cell-cell interactions. The aim this study was investigate the expression pattern endosialin human brain tumors bid decipher its contribution angiogenesis. We generated an antibody specifically recognizing used it 30 specimens immunoblotting...
Objectives: To determine whether narrowband UV-B (NB-UV-B) may mediate its beneficial effect on psoriasis by increasingvitaminDlevels,andtoassesstheeffectofNB-UV-B vitamin D status in patients with wintertime.
Although vitamin D deficiency is considered an environmental factor in multiple sclerosis (MS), the immunological and clinical effects of supplementation remain unclear. We performed a pilot study immunomodulatory healthy individuals (n=4), who took 5000-10,000 IU/day over 15 weeks. After weeks supplementation, serum 25(OH) levels rose significantly from baseline, with corresponding increase IL-10 production by peripheral blood mononuclear cells reduced frequency Th17 cells. These data...