A5061956666- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Estrogen and related hormone effects
- Global Cancer Incidence and Screening
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Protein Degradation and Inhibitors
- Cancer Research and Treatments
- Genomics and Chromatin Dynamics
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- Cancer Treatment and Pharmacology
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Breast Cancer Treatment Studies
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Cell Adhesion Molecules Research
University College Dublin
2015-2024
Children's Hospital of Los Angeles
2024
Twin Cities Orthopedics
2023
University of Minnesota
2023
Bevital (Norway)
2023
Griffith University
2022
Conway School of Landscape Design
2004-2018
Manchester Metropolitan University
2018
University of Ulster
2018
Charitable Infirmary
2014
Ischemia caused by coronary artery disease and myocardial infarction leads to aberrant ventricular remodeling cardiac fibrosis. This occurs partly through accumulation of gene expression changes in resident fibroblasts, resulting an overactive fibrotic phenotype. Long-term adaptation a hypoxic insult is likely require significant modification chromatin structure order maintain the Epigenetic may play important role modulating hypoxia-induced fibrosis within heart. Therefore, aim study was...
BRCA1-mutant breast tumors are typically estrogen receptor alpha (ERα) negative, whereas most sporadic express wild-type BRCA1 and ERα positive. We examined a possible mechanism for the observed ERα-negative phenotype of tumors. used cancer disease–specific microarray to identify transcripts that were differentially expressed between paraffin-embedded samples 17 14 measured mRNA levels 1 (ESR1) (the gene encoding ERα), which was in tumor samples, by quantitative polymerase chain reaction....
Abstract Annually, ovarian cancer (OC) affects 240,000 women worldwide and is the most lethal gynecological malignancy. High‐grade serous OC (HGSOC) common aggressive subtype, characterized by widespread genome changes chromosomal instability consequently poorly responsive to chemotherapy treatment. The objective of this study was investigate role microRNA miR‐433 in cellular response cells paclitaxel We show that stable expression A2780 results induction senescence demonstrated...
Using a specific antiserum (21N) to the c-erbB-2 oncoprotein, total of 405 primary malignant human tumors arising in breast (n = 191), lung 110), colon/rectum 23), bladder 48), prostate and skin 10) were stained immunohistochemically detect those that over-expressed this putative transmembrane receptor. Malignant cells showed intense positivity for oncoprotein 17% carcinomas, 4% colorectal tumors, 2% 1% nonsmall cell carcinomas. No positive staining was evident prostate, skin, or small This...
Manual interpretation of immunohistochemistry (IHC) is a subjective, time-consuming and variable process, with an inherent intra-observer inter-observer variability. Automated image analysis approaches offer the possibility developing rapid, uniform indicators IHC staining. In present article we describe development novel approach for automatically quantifying oestrogen receptor (ER) progesterone (PR) protein expression assessed by in primary breast cancer. Two cohorts cancer patients (n =...
DNA microarrays have the potential to classify tumors according their transcriptome. Tissue (TMAs) facilitate validation of biomarkers by offering a high-throughput approach sample analysis. We reanalyzed high profile breast cancer microarray dataset containing 96 tumor samples using powerful statistical approach, between group analyses. Among genes we identified was centromere protein-F (CENP-F), gene associated with poor prognosis. In published follow-up study, comprising 295 tumour...
Increasing levels of tissue hypoxia have been reported as a natural feature the aging prostate gland and may be risk factor for development cancer. In this study, we used PwR-1E benign epithelial cells an equivalently aged hypoxia-adapted sub-line to identify phenotypic epigenetic consequences chronic in cells. We identified significantly altered cellular phenotype response characterized by increased receptor-mediated apoptotic resistance, induction senescence, invasion secretion IL-1β, IL6,...
Anxiety is associated with elevated levels of the inflammatory cytokine interleukin-6 (IL-6) and an increased risk for diseases aetiology. In cancer, higher IL-6 have been expression epigenetic enzymes DNMT1 Enhancer Zeste Homolog 2 (EZH2). However, relationship between DNA methyltransferases (DNMTs) EZH2 has not previously examined in anxious individuals.Global methylation were measured using Methylflash Methylated Quantification Kit gene DNMT genes (n=25) nonanxious individuals (n=22)...
Triple negative breast cancer (TNBC) is an aggressive subtype with relatively poor clinical outcomes and limited treatment options. Chemotherapy, while killing cells, can result in the generation of highly chemoresistant therapeutic induced senescent (TIS) cells that potentially form stem cell niches resulting metastases. Intriguingly, release significantly more extracellular vesicles (EVs) than non-senescent cells. Our aim was to profile EVs harvested from TIS TNBC compared control identify...
Multiple myeloma (MM) is an incurable cancer that derives pro-survival/proliferative signals from the bone marrow (BM) niche. Novel agents targeting not only cells, but also BM-niche have shown greatest activity in MM. Histone deacetylases (HDACs) are therapeutic targets MM and we previously showed HDAC3 inhibition decreases proliferation both alone co-culture with stromal cells (BMSC). In this study, investigate effects of BMSCs. Using BMSC lines as well patient-derived BMSCs, show...
Epithelial ovarian cancer (EOC) has an innate susceptibility to become chemoresistant. Up 30% of patients do not respond conventional chemotherapy [paclitaxel (Taxol®) in combination with carboplatin] and, those who have initial response, many relapse. Therefore, understanding the molecular mechanisms that regulate cellular chemotherapeutic responses EOC cells potential impact significantly on patient outcome. The mitotic arrest deficiency protein 2 (MAD2), is a centrally important mediator...
Administration of the heavy metal cadmium (Cd) induces ventral body wall defects (VBWD) in chick embryo. In this model, expression most genes involved formation is altered 4h-posttreatment. However, mechanism by which Cd results initiation gene remains unclear. Epigenetic mechanisms can change genome function under exogenous influences. Moreover, one environmental factors that affect epigenomic programming. De novo DNA methylation essential for normal embryogenesis and regulated...
Glycosylation is the most common posttranslational modification of proteins and highly reflective changes in environment a cell. Epigenetic modifications to genome are stably transmitted daughter cells without requirement for genetic sequence alterations. Aberrant regulation both epigenetic programming glycosylation patterning integral aspects carcinogenesis. The objective this study was determine interplay between these two complex cellular processes. We demonstrate that global DNA...
Abstract Background Although omic-based discovery approaches can provide powerful tools for biomarker identification, several reservations have been raised regarding the clinical applicability of gene expression studies, such as their prohibitive cost. However, limited availability antibodies is a key barrier to development lower cost alternative, namely discrete collection immunohistochemistry (IHC)-based biomarkers. The aim this study was use systematic approach generate and screen...
The prognosis of epithelial ovarian cancer is poor in part due to the high frequency chemoresistance. Recent evidence points Toll-like receptor-4 (TLR4), and particularly its adaptor protein MyD88, as one potential mediator this resistance. This study aims provide further that MyD88 positive cells are clinically significant, stem-like reproducibly detectable for purposes prognostic stratification. Expression TLR4 was assessed immunohistochemically 198 paraffin-embedded tissues an embryonal...